Results 251 to 260 of about 44,698 (301)

Expression of Sclerostin scFv and the Effect of Sclerostin scFv on Healing of Osteoporotic Femur Fracture in Rats

Cell Biochemistry and Biophysics, 2013
Osteoporosis is a systemic metabolic disease characterized by low bone mass with deterioration of the bony microstructure which leads to both bone brittleness and increased risk of fracture. Sclerostin is a protein encoded by the SOST gene which is specifically expressed in osteocyte.
Qi, Yao, Jie, Ni, Yu, Hou, Lixiang, Ding, Licheng, Zhang, Hua, Jiang   +5 more
openaire   +2 more sources

Reformatting of scFv Antibodies into the scFv-Fc Format and Their Downstream Purification

2014
The scFv-Fc format allows for rapid characterization of candidate scFvs isolated from phage display libraries before conversion into a full-length IgG. This format offers several advantages over the phage display-derived scFv, including bivalent binding, longer half-life, and Fc-mediated effector functions.
Emil, Bujak, Mattia, Matasci, Dario, Neri, Sarah, Wulhfard   +3 more
openaire   +2 more sources

Enzymatic Assembly for scFv Library Construction

2017
Recombinant monoclonal antibodies can be established by displaying single-chain variable fragment (scFv) antibody libraries on phages and then biopanning against the target. For constructing superior scFv libraries, antibody light-chain variable region (VL) and heavy-chain variable region (VH) fragments must be assembled into scFvs without loss of ...
Mieko, Kato, Yoshiro, Hanyu
openaire   +2 more sources

Screening for scFv-fragments that are stable and active in the cytosol

Human Antibodies, 2020
Intrabodies are antibodies that are not secreted but bind to their antigens inside the cell producing them. Intrabodies targeting antigens in the endoplasmatic reticulum were successfully used in vitro and in vivo. However, many target antigens interesting for research or therapy are located in the reducing environment of the cytosol, where correct ...
Stefanie, Behring, Robert, Hänsch, Saskia, Helmsing, Thomas, Schirrmann, Maren, Schubert   +4 more
openaire   +2 more sources

Parallelized Microscale Expression of Soluble scFv

2019
Antibody phage display is a key technology to generate recombinant, mainly human, antibodies for diagnostic and therapy, but also as tools for basic research. After antibody selection by "panning," a crucial step is the screening of monoclonal binders to isolate those which show antigen specificity.
Giulio, Russo, Viola, Fühner, André, Frenzel, Michael, Hust, Stefan, Dübel   +4 more
openaire   +2 more sources

T7 displayed peptides as targets for selecting peptide specific scFvs from M13 scFv display libraries

Journal of Immunological Methods, 2001
M13 scFv display libraries are frontline research tools due to the rapidity in which target binding scFv can be obtained. Simple modifications of the standard affinity selection and amplification protocol allow for multiple targets to be panned simultaneously.
J, Castillo, B, Goodson, J, Winter
openaire   +2 more sources

ScFv-CH3 and scFv-Fc Fusion Proteins

2001
Earlier chapters (Chapters 2–16) have presented methods for producing small engineered antibodies (notably, scFv) from hybridomas or through selection from combinatorial libraries. It is now possible to generate fragments with a vast spectrum of specificities, including against targets previously inaccessible through hybridoma technology.
openaire   +1 more source

Efficient display of scFv antibodies on bacteriophage lambda

Journal of Immunological Methods, 2006
In the present work we demonstrate the efficient display of functional scFv antibodies on the bacteriophage lambda capsid. A single-chain (scFv) anti-CEA antibody gene was cloned in two different vectors to obtain fusion of the scFv antibody to the N- or C-terminus of the bacteriophage lambda capsid protein D (gpD). Lambda bacteriophage assembly occurs
VACCARO P, PAVONI E, MONTERIU' G, PUCCI A, FELICI, Franco, MINENKOVA O.   +5 more
openaire   +3 more sources

High avidity scFv multimers; diabodies and triabodies

Journal of Immunological Methods, 1999
Multivalent recombinant antibody fragments provide high binding avidity and unique specificity to a wide range of target antigens and haptens. This review describes how careful choice of linker length between V-domains creates new types of Fv modules with size, flexibility and valency suited to in vivo imaging and therapy. Further, we review the design
P J, Hudson, A A, Kortt
openaire   +2 more sources

Isolation and Validation of Anti-B7-H4 scFvs from an Ovarian Cancer scFv Yeast-Display Library

2015
B7-H4 (VTCN1, B7x, B7s) is an inhibitory modulator of T-cell response implicated in antigen tolerization. As such, B7-H4 is an immune checkpoint of potential therapeutic interest. To generate anti-B7-H4 targeting reagents, we isolated antibodies by differential cell screening of a yeast-display library of recombinant antibodies (scFvs) derived from ...
Dangaj D, Scholler N
openaire   +3 more sources