Results 261 to 270 of about 44,698 (301)
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2001
Systemic administration of interleukin-2 (IL-2) has been used for immunotherapy of a variety of human malignancies. However, its rapid blood clearance, that does not allow optimal concentration at the tumorsite, and severe toxicity at high dosage have limited its efficacy in vivo.
Cecilia Melani, Daniela Nicosia
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Systemic administration of interleukin-2 (IL-2) has been used for immunotherapy of a variety of human malignancies. However, its rapid blood clearance, that does not allow optimal concentration at the tumorsite, and severe toxicity at high dosage have limited its efficacy in vivo.
Cecilia Melani, Daniela Nicosia
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Preparation and characterization of scFv for affinity purification of reteplase
Journal of Biochemical and Biophysical Methods, 2006The work was to explore the feasibility of protein affinity purification using ligand isolated from phage library. Reteplase was used as the model protein and a humanized semi-synthetic single chain fragment variable phage library as the source of ligand. After four rounds of biopanning, reteplase-specific phage clones were greatly enriched.
Zhigang, Guo +6 more
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[Construction of human phage-displayed scFv library and selection of the ScFv against rabies virus].
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology, 2006To construct a human phage displayed single chain Fv antibody (scFv) library and screen specific scFv against rabies virus.Using a phage-display technique, IgVH and IgVL genes were cloned from peripheral blood lymphocytes from three donors immunized with the WISTAR PM strain vaccine.
Xiao-ling, Zhao +4 more
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High-Level Periplasmic Expression and Purificationof scFvs
2003The isolation of recombinant antibodies by phage display naturally leads to experiments to evaluate their biological and immunological properties. Although crude preparations may have their value in initial studies, the need often exists for highly purified protein that can be tested in vivo.
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Production of Bispecific Antibodies: Diabodies and Tandem scFv
2012Recombinant bispecific antibodies have many different applications; especially promising is their therapeutic potential due to their ability to retarget an effector molecule or a cell to a disease-related target structure. In the last years, many formats have been developed: two commonly used are the bispecific diabody and the tandem scFv.
Nora, Hornig, Aline, Färber-Schwarz
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The Isolation of scFvs Against Small Target Molecules
2009Phage display has the capacity to rapidly isolate recombinant antibodies against protein targets and other molecules of significant size. However, there is no obvious lower limit to the power of the selection methods: this chapter describes how the techniques of phage display can be adapted to allow the isolation of antibodies against very small ...
Iain, Shaw, Marian, Kane
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Construction of Human Immune and Naive scFv Libraries
2017Antibody phage display is the most commonly used in vitro selection technology for the generation of human recombinant antibodies and has yielded thousands of useful antibodies for research, diagnostics, and therapy. The prerequisite for successful generation of antibodies using phage display is the construction of high-quality antibody gene libraries.
Jonas, Kügler +3 more
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2010
Aim. To develop approach for detection of scFv and their complexes with antigens. Methods. The fusion proteins, which include sequences of scFv and staphylococcal protein A, were constructed and the obtained bifunctional molecules were immunochemically analysed. Results. It was shown, that scFv fused with protein A and their complexes with antigens are
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Aim. To develop approach for detection of scFv and their complexes with antigens. Methods. The fusion proteins, which include sequences of scFv and staphylococcal protein A, were constructed and the obtained bifunctional molecules were immunochemically analysed. Results. It was shown, that scFv fused with protein A and their complexes with antigens are
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Expression of scFvs and scFv Fusion Proteins in Eukaryotic Cells
2003Michelle, de Graaf +3 more
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