Results 271 to 280 of about 44,698 (301)

Selection of Stable scFv Antibodies by Phage Display

2012
ScFv fragments are popular recombinant antibody formats but often suffer from limited stability. Phage display is a powerful tool in antibody engineering and applicable also for stability selection. ScFv variants with improved stability can be selected from large randomly mutated phage displayed libraries with a specific antigen after the unstable ...
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[Construction of anti-B7-H4-scFv library and screening and identification of anti-B7-H4-scFv].

Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology, 2017
Objective To construct the ribosome display library of anti-B7-H4 extracellular domain, and select the antibody with high specificity. Methods The cDNA of B7-H4 extracellular domain was amplified from A549 cells by reverse transcription PCR (RT-PCR).
Luanluan, Shao, Chaochao, Xu, Hongshuai, Ji, Weiping, Mao, Yingying, Wang, Xiaoqian, Liu, Yanyan, Zhu   +6 more
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Mechanisms of Action and Limitations of Monoclonal Antibodies and Single Chain Fragment Variable (scFv) in the Treatment of Cancer

Biomedicines, 2023
Berenice Illades-Aguiar, Eugenia Flores-Alfaro, Marco Antonio Leyva-Vazquez   +2 more
exaly  

Secretion of scFv Antibody Fragments

1998
H, Gram, R, Schmitz, R, Ridder
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Engineering scFvs for Improved Stability

2003
Partha S, Chowdhury, George, Vasmatzis
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Targeted nanomedicine with anti-EGFR scFv for siRNA delivery into triple negative breast cancer cells

European Journal of Pharmaceutics and Biopharmaceutics, 2020
Phuoc Vinh Nguyen, Katel Hervé-Aubert, Stephanie David   +2 more
exaly  

Soluble expression of single-chain variable fragment (scFv) in Escherichia coli using superfolder green fluorescent protein as fusion partner

Applied Microbiology and Biotechnology, 2019
Fei Wang, Xiaolan Yu
exaly  

High-level expression, purification, and characterization of bifunctional ScFv-9R fusion protein

Applied Microbiology and Biotechnology, 2014
Yechen Xiao
exaly  

?????????????????????? ?????????????????? ?????????? ScFv-CBD ?? ???????????? ?????????????????? Escherichia ????li

2019
An inexpensive and effective refolding method for ScFv-CBD fusion protein recovery from E. coli inclusion bodies has been developed. The principle of the proposed method is the stepwise dilution of solubilized ScFv-CBD in the presence of 6 M guanidine hydrochloride with refolding buffer containing L-arginine and glutathione.
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ScFv

2011
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