Results 51 to 60 of about 33,254 (223)
Abstract The qualified presumption of safety (QPS) process was developed to provide a harmonised safety assessment approach to support EFSA Scientific Panels and Units. The QPS approach assesses the taxonomic identity, body of relevant knowledge and safety of microorganisms intentionally added to the food and feed chain.
EFSA Panel on Biological Hazards (BIOHAZ) +28 more
wiley +1 more source
Rad27/FEN1 prevents accumulation of Okazaki fragments and ribosomal DNA copy number changes
The budding yeast Rad27 is a structure‐specific endonuclease. Here, the authors reveal that Rad27 is crucial for maintaining the stability of the ribosomal RNA gene (rDNA) region. Rad27 deficiency leads to the accumulation of Okazaki fragments and changes in rDNA copy number.
Tsugumi Yamaji +3 more
wiley +1 more source
Population genomics of the fission yeast Schizosaccharomyces pombe. [PDF]
The fission yeast Schizosaccharomyces pombe has been widely used as a model eukaryote to study a diverse range of biological processes. However, population genetic studies of this species have been limited to date, and we know very little about the ...
Jeffrey A Fawcett +10 more
doaj +1 more source
Plouviez et al. give a perspective on the terminology used for describing the mechanisms of polyphosphate accumulation in microalgae and yeast. Their differentiation of mechanisms is based on environmental P availability, polyP chemistry, genetics, or cellular function as described in recent literature.
Maxence Plouviez +7 more
wiley +1 more source
DNA Preparation from Schizosaccharomyces pombe [PDF]
This protocol includes two methods for genomic DNA preparation from Schizosaccharomyces pombe. The first is a quick method for preparation of DNA suitable for polymerase chain reaction (PCR) genotyping. The second, longer method yields high-quality DNA that can be used for amplification of targeting cassettes.
Assen, Roguev +2 more
openaire +2 more sources
Optimisation of the Schizosaccharomyces pombe urg1 expression system. [PDF]
The ability to study protein function in vivo often relies on systems that regulate the presence and absence of the protein of interest. Two limitations for previously described transcriptional control systems that are used to regulate protein expression
Adam T Watson +6 more
doaj +1 more source
ABSTRACT Cells have an intrinsic ability to rapidly respond to environmental change to regulate cell cycle progression and membrane organisation, thereby affecting cell growth and division. The actin cytoskeleton is a highly dynamic complex of proteins that can rapidly reorganise to change the growth pattern of a cell.
Holly R. Brooker +7 more
wiley +1 more source
Multifunctional cytokinesis genes in schizosaccharomyces pombes [PDF]
The proper division of cells is essential for the production of viable daughter cells. In plants and fungi, the dividing cell produces a cross-wall or septum that bisects the cytoplasm. For separation of the daughter cells, the septum has to be cleaved. To study the regulation of this process, we isolated mutants defective in septum cleavage.
Grallert, Ágnes +4 more
openaire +3 more sources
Genomics and other large-scale analyses have drawn increasing attention to the potential impacts of transposable elements (TEs) on their host genomes. However, it remains challenging to transition from identifying potential roles to clearly demonstrating
Patrick H. Maxwell
doaj +1 more source
Engineering Strategies for Fungal Cell Disruption in Biotechnological Applications
ABSTRACT Fungal cell disruption plays a critical role in unlocking a wide range of high‐value intracellular products such as lipids, proteins, pigments, and bioactive compounds. However, lysing fungal cells is far more challenging than breaking bacterial or algal cells due to their robust and highly structured cell walls.
Bhagyeshri Ulhas Mantri +2 more
wiley +1 more source

