Results 121 to 130 of about 183,994 (309)

scRNA-seq protocol v1

scRNA-seq protocol for ESC DE differentiation time course
Renhe Luo, Michael Beer, Danwei Huangfu
openaire   +1 more source

constclust: Consistent clusters for scRNA-seq

, 2019
Unsupervised clustering to identify distinct cell types is a crucial early step in analyses of scRNAseq data. Current methods are dependent on a number of parameters, whose effect on the resulting solution's accuracy and reproducibility are frequently uncharacterized – resulting in ad-hoc selection by users. constclust is a novel meta-clustering method
Virshup, Isaac, Jarny Choi, Bower, Neil, Hogan, Ben, Kim-Anh Le Cao, Wells, Christine   +5 more
openaire   +1 more source

Simplifying complex scRNA-seq data with metacells

, 2022
Single-cell RNA sequencing (scRNA-seq) provides unique opportunities for studying heterogeneous cells populations. However, in-depth single-cell transcriptomic characterization often requires profiling tens to hundreds of thousands of cells.
BILOUS, Mariia
core  

SCdenoise: a reference-based scRNA-seq denoising method using semi-supervised learning

, 2022
scRNA-seq is a promising technology to perform unbiased, high-throughput, and high-resolution transcriptome analysis at single-cell resolution. The raw data usually suffers from noise and low quality, such as dropout events, which hinder downstream ...
Liu, Y, Zhong, F, Zeng, Y, Yang, Y
core   +1 more source

Icariin Enhances the Enzymatic Activity of N‐acetylgalactosaminidase to Augment Akkermansia Abundance in Gut Microbiota for Improved PD‐1 Blockade Efficacy in Tumor Suppression

Advanced Science, EarlyView.
Icariin promoted the growth of Akk by enhancing the activity of N‐acetylgalactosaminidase (Amuc_0920), which enhanced mucin utilization and provided a favorable nutrient environment for bacterial growth. This icariin‐mediated enrichment of Akk further reshaped the tumor microenvironment and promoted CD8+ T cell infiltration, ultimately synergizing with
Shuangying Qiao, Liu Yang, Haibang Hao, Qiuxia Ding, Feng Ding, Zheng Chen, Jinfang Zhang, Yun He, Meng Li, Jun Xu, Chao Wang, Aiping Lu, Fangfei Li   +12 more
wiley   +1 more source

O‐GlcNAcylated TAP1 Impairs Antigen Presentation and Promotes Immune Evasion in Bladder Cancer

Advanced Science, EarlyView.
This article unveils a critical mechanism of immune evasion in bladder cancer, which the O‐GlcNAc modification of TAP1 disrupts antigen presentation. This modification triggers HLA‐A degradation, shielding tumor cells from CD8+ T cell attack. The findings highlight targeting this pathway as a promising therapeutic avenue to sensitize tumors to ...
Jinpeng Wu, Xueting Ren, Zhen Zhai, Lu Wang, Liang Liang, Zengqi Tan, Jiazhen Zhao, Yuxuan Han, Yuhan Li, Feng Guan, Xiang Li   +10 more
wiley   +1 more source

scRNA-seq raw data for BCL9 in cDC1

We performed single-cell sequencing (scRNA-seq) of tumors and TdLNs from B16-OVA tumor-bearing Bcl9/Bcl9l deficient mice (n = 3) and wild type mice (n = 3).
Zhongen Wu (16888458)
core   +1 more source

Tumor‐Derived LAMB3 Drives Immunosuppressive LRRC15+ Fibroblast Formation During Pancreatic Ductal Adenocarcinoma Development

Advanced Science, EarlyView.
A single‐cell atlas of pancreatic ductal adenocarcinoma development reveals progressive ductal‐fibroblast‐immune crosstalk. Tumor‐derived LAMB3 drives the formation of immunosuppressive LRRC15+ fibroblasts through the ITGB1/FAK/MAPK/FOSL2 signaling. Glycolytic reprogramming upregulates LAMB3 and correlates with LRRC15+ fibroblast enrichment.
Xuqing Shi, Hangqi Liu, Jianru Sun, Xiaoding Liu, Xinping Jv, Longyun Chen, Yuhan Zhang, Hui Zhang, Xudong Xing, Ruiyu Li, Xinyi Ke, Jun Wang, Xianglin Yin, Bohan Liu, Qixian Liu, Yuan Wang, Junliang Lu, Shiyi Liu, Junyi Pang, Yumeng Cai, Menghua Dai, Fan Bai, Huanwen Wu, Zhiyong Liang   +23 more
wiley   +1 more source

Statistical analysis of RNA-seq and scRNA-seq expression data

, 2018
RNA-seq is a popular technique that utilizes next-generation sequencing to obtain transcriptome information from a cell population. It generates short sequences of reads from the transcriptome, which can be utilized for gene annotation, expression ...
葉信恆, Yip, Shun-hang
core   +1 more source

A Microbial Lipid‐ATP Synthase Axis Fuels NK Cell Antitumor Activity

Advanced Science, EarlyView.
This study focuses on the mechanism by which gut microbiota‐derived outer membrane vesicles (OMVs) regulate NK cell antitumor activity. B. intestinalis is identified to decrease extra‐intestinal tumor growth via its OMVs enriched in sphingosine (SP).
Kaiyuan Yu, Xinyu Sun, Wanxia Ma, Jianming Yang, Xuan Sun, Lisong Zhang, Yumeng Liu, Tianshu Ren, Qi Wang, Jingyu Wang, Xiao Li, Xianping Peng, Liu Yang, Junqiang Lv, Zhi Yao, Zhi‐Song Zhang, Quan Wang   +16 more
wiley   +1 more source