The rumen microbial metaproteome as revealed by SDS-PAGE. [PDF]
BMC Microbiol, 2017 Ruminal digestion is carried out by large numbers of bacteria, archaea, protozoa and fungi. Understanding the microbiota is important because ruminal fermentation dictates the efficiency of feed utilisation by the animal and is also responsible for major emissions of the greenhouse gas, methane.
Snelling TJ, Wallace RJ.
europepmc +11 more sources
Molluscan Shell Matrix Characterization by Preparative SDS-PAGE [PDF]
The Scientific World Journal, 2003 The glycoproteinaceous constituents of molluscan shell matrices usually resist chromatographical fractionation. We describe a protocol that overcomes this difficulty and permits collection of a large amount of shell proteins for further in vitro ...
Frederic Marin
doaj +9 more sources
SDS-PAGE technique as biomarker for fish toxicological studies [PDF]
Toxicology Reports, 2018 Although many studies on the hematological and biochemical parameters in fishes have been done, still there are some shortage in the estimation and evaluation of the baseline’s values of marine and freshwater fishes.
Ola I. Muhammad +3 more
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Preparative SDS PAGE as an Alternative to His-Tag Purification of Recombinant Amelogenin. [PDF]
Front Physiol, 2017 Recombinant protein technology provides an invaluable source of proteins for use in structure-function studies, as immunogens, and in the development of therapeutics.
Gabe CM, Brookes SJ, Kirkham J.
europepmc +6 more sources
Monitoring Protein Splicing Using In-gel Fluorescence Immediately Following SDS-PAGE. [PDF]
Bio Protoc, 2021 Inteins garner significant interest from both basic and applied researchers due to their unique catalytic abilities. Herein, we describe a protocol for accurately monitoring protein splicing without purification using in-gel fluorescence immediately ...
Weinberger Ii J, Lennon CW.
europepmc +3 more sources
The Carboxyl-Terminal Amino Acids Render Pro-Human LC3B Migration Similar to Lipidated LC3B in SDS-PAGE [PDF]
PLoS ONE, 2013 LC3 is widely used marker for macroautophagy assays. After translation pro-LC3 is processed by Atg4 to expose C-terminal glycine residue for downstream conjugation reactions to accomplish the conversion of LC3-I to LC3-II. SDS-PAGE based Western blot (Wb)
Wei Wang +4 more
semanticscholar +4 more sources
Peculiarities of SDS-PAGE of Titin/Connectin [PDF]
, 2018 Titin (also known as connectin) is a giant elastic protein of striated and smooth muscles of vertebrates. The molecular weight of its isoforms is 3.0–3.7 MDa in striated muscles and 0.5–2.0 MDa in smooth muscles.
Zoya A. Podlubnaya, Ivan M. Vikhlyantsev
openaire +4 more sources
SDS-PAGE to Immunoblot in One Hour. [PDF]
Methods Mol Biol, 2015 An ultra-rapid method for electrophoresing proteins on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, transfer of proteins to nitrocellulose membranes, and immunoblotting is described here. Electrophoresis of the autoantigens La and Ro60, as well as molecular weight standards on a 4-20 % gradient gel, was performed in about 10 min using ...
Kurien BT +3 more
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2D Diagonal Redox SDS-PAGE of Proteins
Bio-Protocol, 2013 2D diagonal redox SDS-PAGE of proteins is used to detect intramolecular or intermolecular disulfide bridges using Chlamydomonas in this example (Stroeher and Dietz, 2008; Schwarz et al., 2012).
Christian Schwarz, Joerg Nickelsen
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Detection of the basement membrane-degrading proteolytic activity of Paracoccidioides brasiliensis after SDS-PAGE using agarose overlays containing Abz-MKALTLQ-EDDnp [PDF]
, 1999 We have characterized, in the Paracoccidioides brasiliensis yeast phase, an exocellular SH-dependent serine proteinase activity against Abz-MKRLTL-EDDnp and analogous fluorescent-quenched peptides, and showed that it is also active against constituents ...
Rosana Puccia +4 more
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