Results 71 to 80 of about 670,402 (263)

DDX3X induces mesenchymal transition of endothelial cells by disrupting BMPR2 signaling

FEBS Open Bio, EarlyView.
Elevated DDX3X expression led to downregulation of BMPR2, a key regulator of endothelial homeostasis and function. Our co‐immunoprecipitation assays further demonstrated a molecular interaction between DDX3X and BMPR2. Notably, DDX3X promoted lysosomal degradation of BMPR2, thereby impairing its downstream signaling and facilitating endothelial‐to ...
Yu Zhang, Jing Wang, De‐Hui Qian, Yang‐Fan Lv, Tian‐Le Cheng, Da‐Peng Wang, Jing Zhang, Ye Fan   +7 more
wiley   +1 more source

ATP13A2 is involved in intracellular polyamine transport in lung epithelial cells

FEBS Open Bio, EarlyView.
Spermidine transport in lung epithelial cells involves the polyamine transporter ATP13A2. Cell proliferation is associated with the upregulation of ATP13A2. Polyamines are present in all living cells and are implicated in various crucial cellular processes such as proliferation, apoptosis and autophagy.
Yuta Hatori, Kohei Kawabata, Takanori Kubo, Takeo Kitazawa, Sae Kanai, Madoka Iwashita, Ami Hayashi, Hiroyuki Nishi, Mikihisa Takano   +8 more
wiley   +1 more source

Metformin promotes mitochondrial integrity through AMPK‐signaling in Leber's hereditary optic neuropathy

FEBS Open Bio, EarlyView.
Metformin mediates mitochondrial quality control in Leber's hereditary optic neuropathy (LHON) fibroblasts carrying mtDNA mutations. At therapeutic levels, metformin activates AMPK signaling to restore mitochondrial dynamics by promoting fusion and restraining fission, while preserving mitochondrial mass, enhancing autophagy/mitophagy and biogenesis ...
Chatnapa Panusatid, Rapasviranda Soiyangsuk, Maneeluck Tanadjindarat, Chayanon Peerapittayamongkol   +3 more
wiley   +1 more source

The Identification of proteins present in culture filtrare of Mycobacterium tuberculosis DT and comparison with human tuberculin proteins by electrophoretic methods

Medical Laboratory Journal, 2012
Background and objectives: Tuberculin is the proteins existed in tuberculosis culture medium which precipitated by trichloroacetic acid (TCA) or ammonium sulfate. Tuberculin is used for diagnosis of Tuberculosis.
Sadeghi D (MSc), Mosavari N (PhD), Rafiee B (MSc), Mohamad Taheri M (MSc), Dashtipour Sh (BSc), Zare A (PhD), Ghahremanlo E (MSc), Tebyanian M (PhD)   +7 more
doaj  

PERFIL PROTEICO DO LÍQUIDO FOLICULAR COLETADO DE OVÁRIOS EM DIFERENTES FASES DO CICLO ESTRAL DE BOVINOS

Colloquium Agrariae, 2012
The female gamete’s microenvironment is the follicular fluid. The proteins in the follicular fluid must interfere in the oocyte quality. The objective of this study was to verify the protein profile in the follicular fluid of different phases of ...
Renato Weller Ribeiro, Francislaine Aneliza Garcia Santos, Caliê Castilho, José Giometti, Luciana Machado Guaberto, Ana Claudia Ambiel, Deyvid Parreira Vieira, Cyntia Monteiro dos Santos, Ines Cristina Giometti   +8 more
doaj   +1 more source

SDS-PAGE gel electrophoresis v3 [PDF]

, 2020
SDS-PAGE gel electrophoresis protocol for analyzing samples from plant leaf tissue via immunofluorescence. In this protocol no Coomassie blue is added to samples, the reason is that this interferes with the fluorescent signal during immunoblot. Instead, samples have already been prepared in Laemmli buffer (minus coomassie, protein extraction procedure),
openaire   +1 more source

FGFR Like1 drives esophageal cancer progression via EMT, PI3K/Akt, and notch signalling: insights from clinical data and next‐generation sequencing analysis

FEBS Open Bio, EarlyView.
Clinical analysis reveals significant dysregulation of FGFRL1 in esophageal cancer (EC) patients. RNAi‐coupled next‐generation sequencing (NGS) and in vitro study reveal FGFRL1‐mediated EC progression via EMT, PI3K/Akt, and Notch pathways. Functional assays confirm its role in tumor growth, migration, and invasion.
Aprajita Srivastava, Anoop Saraya, Deepak Gunjan, Rinu Sharma   +3 more
wiley   +1 more source

LowC SDS-PAGE v1

, 2017
SDS-PAGEusing gels with low crosslinking of acrylamide and bisacrylamide. This protocol can be used to separate phosphorylation forms of KaiC proteins.
Anika Wiegard, not provided Ilka Maria Axmann   +1 more
openaire   +1 more source

KLK7 overexpression promotes an aggressive phenotype and facilitates peritoneal dissemination in colorectal cancer cells

FEBS Open Bio, EarlyView.
KLK7, a tissue kallikrein‐related peptidase, is elevated in advanced colorectal cancer and associated with shorter survival. High KLK7 levels in ascites correlate with peritoneal metastasis. In mice, KLK7 overexpression increases metastasis. In vitro, KLK7 enhances cancer cell proliferation, migration, adhesion, and spheroid formation, driving ...
Yosr Z. Haffani, Tobias Dreyer, Meriem Naim, Rea Lo Dico, Natalia A. Ignatenko, Viktor Magdolen, Dalila Darmoul   +6 more
wiley   +1 more source

Primary proteolysis of white brined goat cheese monitored by high molarity Tris buffer SDS- PAGE system

Mljekarstvo, 2013
The aim of this work was to investigate primary proteolysis of white brined goat cheese prepared from raw milk and to correlate with the results obtained with high-molarity Tris buffer electrophoretic system.
Milenko Smiljanić, Mirjana B. Pešić, Miroljub B. Barać, Sladjana P. Stanojević, Snežana T. Jovanović, Ognjen D. Maćej   +5 more
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