Results 221 to 230 of about 684,938 (261)

SDS-PAGE and Western Blotting

2000
Proteins can be separated according to their molecular sizes and charges, since these factors will determine the speed at which they will travel through a gel. The SDS-PAGE method involves the denaturation of proteins with the detergent sodium dodecyl sulfate (SDS) and the use of an electric current to pull them through a polyacrylamide gel, a process ...
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SDS-PAGE and Western Blotting Techniques

2003
The goal of Western blotting, or more correctly, immunoblotting, is to identify with a specific antibody a particular antigen within a complex mixture of proteins that has been fractionated in a polyacrylamide gel and immobilized onto a membrane. Immunoblotting can be used to determine a number of important characteristics of protein antigens-the ...
C, Blancher, A, Jones
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Electrophoresis of Peptides (Tricine-SDS-PAGE)

Cold Spring Harbor Protocols, 2006
INTRODUCTION Typical Laemmli gel systems, which use glycine in the running buffer, are capable of resolving proteins in the molecular mass range of ~200,000 Da down to ~3000 Da. In this protocol, the standard tricine gel system is described, in which tricine is substituted for glycine.
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SDS‐Polyacrylamide Gel Electrophoresis (SDS‐PAGE) of Proteins

Current Protocols in Microbiology, 2011
AbstractElectrophoresis is a method by which a complex mixture of proteins can be separated. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS‐PAGE) is a technique used to move charged molecules through a gel matrix by means of an electric current.
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A comparative study of CE‐SDS, SDS‐PAGE, and Simple Western: Influences of sample preparation on molecular weight determination of proteins

Electrophoresis, 2021
Finja Krebs, Hermann Wätzig, Imke Oltmann-Norden   +2 more
exaly  

A comparative study of CE‐SDS, SDS‐PAGE, and Simple Western—Precision, repeatability, and apparent molecular mass shifts by glycosylation

Electrophoresis, 2021
Finja Krebs, Hermann Wätzig, Imke Oltmann-Norden   +2 more
exaly  

Improving SDS-PAGE method for monoclonal antibodies: The advantages of Tris-Acetate over Tris-Glycine SDS-PAGE system and comparison with CE-SDS method

Protein Expression and Purification, 2021
Hossein Behrouz
exaly  

Distinction between glycomacropeptide and β-lactoglobulin with ‘stains all’ dye on tricine SDS-PAGE gels

Food Chemistry, 2021
Rajan Sharma, Bimlesh Mann, Kamal Gandhi   +2 more
exaly  

SDS-PAGE Analysis

2023
Kannan Harithpriya, Goutham V. Ganesh, Dhamodharan Umapathy, K.M. Ramkumar   +3 more
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SDS-PAGE

2011
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