Results 41 to 50 of about 4,922,198 (308)
, 2017 Separation by SDS-PAGE of the digestion mixture of proteins after treatment with chymotrypsin. Proteins were digested at a mass ratio of protein to protease of 200:1 for 30 min at 30°C (left panel) and 20:1, overnight at 30°C (right panel).
José M. Delfino (736126) +3 more
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FEBS Letters, EarlyView.We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace +6 more
wiley +1 more source
GLUTENIN LOCI VARIABILITY OF CROATIAN WHEAT GERMPLASM [PDF]
Poljoprivreda, 2012 Glutenins loci were used for variability estimation in 50 varieties of hexaploid winter wheat originated from Croatian breeding centres. Polyacrylamide gel electrophoresis (PAGE) in presence of sodium dodecyl sulphate (SDS) was used for determination of ...
Ivana Rukavina +4 more
doaj
SDS-PAGE and Western blot analysis of recombinant IL-18.
, 2016 (A) SDS-PAGE analysis of secreted proteins from the P. pastoris KM71 before purification. (B) SDS-PAGE analysis of recombinant IL-18 after purification with HisTrap affinity column, (C) Western blot analysis of recombinant IL-18 protein. All samples were
Varomyalin Tipmanee (2113162) +5 more
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Structural insights and therapeutic targets in Acinetobacter baumannii capsule biosynthesis
FEBS Letters, EarlyView.Hypervirulent KL49 A. baumannii's capsular polysaccharide contains the nonulosonic acid 8‐epi‐Leg5,7Ac2, synthesized by epimerization via ElaA, ElaB, and ElaC. Crystal structures of ElaA, ElaB, and ElaC reveal their role in CMP‐Leg5,7Ac2 synthesis and regioselective C8 epimerization.
Woo Cheol Lee +7 more
wiley +1 more source
SDS-PAGE analysis of the purified IgM and IgY.
, 2016 The IgM and IgY containing fractions were concentrated using a 100 kDa cut off centrifugal filter (Millipore), and the protein composition determined by SDS-PAGE. The left panel shows proteins separated and visualised using Coomassie staining on a 5% SDS-
Olli Vapalahti (87344) +4 more
core +1 more source
FEBS Letters, EarlyView.CT10 regulator of kinase (CRK) and CRK‐Like (CRKL) are signaling adaptors driving cell adhesion, motility, differentiation, and proliferation. SH2‐domain containing (SH) proteins are enriched in YXXP motifs which when phosphorylated create preferred binding sites for CRK family SH2 domains.
Phoebe M. Cousens +8 more
wiley +1 more source
Journal of Genetic Engineering and Biotechnology, 2016 Somatic chromosome study from root tip cells using the squash technique of the cytological method and seed protein profile of 5 varieties of Lens culinaris (Lentil) through SDS–PAGE were investigated.
Tuhin Pal +3 more
doaj +1 more source
FEBS Letters, EarlyView.The process of internalization of the Shiga toxin A subunit via formation of a complex with the Shiga toxin B subunit, which specifically binds to the Gb3 receptor. The peptide is designed to act as a carrier of drugs into cancer cells. Here, we explored the potential of peptides derived from the catalytic A subunit of Shiga toxin (STxA) to be drug ...
Giulia Opassi +6 more
wiley +1 more source
Avaliação das propriedades físico-químicas, reológicas e do perfil eletroforético por SDS-PAGE de queijo minas frescal [PDF]
, 2015 Tese (doutorado) - Universidade Federal de Santa Catarina, Centro de Ciências Agrárias, Programa de Pós-Graduação em Ciência dos Alimentos, Florianópolis, 2015.O queijo Minas Frescal é um dos produtos lácteos mais consumidos no Brasil, contudo o ...
Magenis, Renata Bongiolo
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