Results 21 to 30 of about 684,938 (261)
FEBS Letters, EarlyView.We identified a systemic, progressive loss of protein S‐glutathionylation—detected by nonreducing western blotting—alongside dysregulation of glutathione‐cycle enzymes in both neuronal and peripheral tissues of Taiwanese SMA mice. These alterations were partially rescued by SMN antisense oligonucleotide therapy, revealing persistent redox imbalance as ...
Sofia Vrettou, Brunhilde Wirth
wiley +1 more source
, 2020 SDS-PAGE is an analytical technique used to separate proteins based on their molecular weight using electrophoresis. Peptides migrate faster due to less resistance from the gel matrix. The peptide used in this experiment is characterised by a short length (molecular weight is less than 3kDa), so we have used a very dense (20%) gel in order to get a ...
openaire +1 more source
FEBS Letters, EarlyView.Structural and biochemical characterisations show that the planar cell polarity (PCP) protein Inturned harbours a unique PDZ‐like domain that does not bind canonical PDZ‐binding motifs (PBMs) like that of another PCP protein Vangl2. In contrast, the apical‐basal polarity protein Scribble contains four PDZ domains that bind Vangl2, but one PDZ domain ...
Stephan Wilmes +4 more
wiley +1 more source
Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Veterinary Medicine, 2017 The milk’s proteins provide nutritional and biologically active values, essential in human and animal nutrition. In the case of goat milk, the proteins’ concentration and quality represent basic indices for the evaluation of the nutritional and ...
Alina NASALEAN +4 more
doaj +1 more source
Calpain small subunit homodimerization is robust and calcium‐independent
FEBS Letters, EarlyView.Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy +4 more
wiley +1 more source
Structural insights into an engineered feruloyl esterase with improved MHET degrading properties
FEBS Letters, EarlyView.A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa +5 more
wiley +1 more source
Estimation of Protease Enzyme Bioactivity Isolated from Bacterial Strains [PDF]
Records of Pharmaceutical & Biomedical SciencesFifteen bacterial strains were isolated from soil samples collected from Northern East region of the delta Egypt. Bacterial strains were screened for proteolytic activity on saline skim milk agar medium where the most potent protease producer strain was ...
Reham Elkased
doaj +1 more source
Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation
FEBS Letters, EarlyView.Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz +11 more
wiley +1 more source
GLUTENIN LOCI VARIABILITY OF CROATIAN WHEAT GERMPLASM [PDF]
Poljoprivreda, 2012 Glutenins loci were used for variability estimation in 50 varieties of hexaploid winter wheat originated from Croatian breeding centres. Polyacrylamide gel electrophoresis (PAGE) in presence of sodium dodecyl sulphate (SDS) was used for determination of ...
Ivana Rukavina +4 more
doaj
Journal of Genetic Engineering and Biotechnology, 2016 Somatic chromosome study from root tip cells using the squash technique of the cytological method and seed protein profile of 5 varieties of Lens culinaris (Lentil) through SDS–PAGE were investigated.
Tuhin Pal +3 more
doaj +1 more source