Results 21 to 30 of about 4,922,198 (308)

SDS-PAGE of purified recombinant proteins.

, 2020
A) RiAFP-m9 appears at a larger apparent mass (~23 kDa) than the theoretical mass (17 kDa) on SDS-PAGE. The molecular weight of the purified protein is verified by ESI-MS (17 kDa). B) SBAFP-CT runs as expected at ~20 kDa on SDS-PAGE.
Maria D. R. Peralta (4568749), Daniel L. Cox (622106), Zeyu Peng (647173), Michael D. Toney (1683499)   +3 more
core   +1 more source

Seed protein electrophoresis for assessment of genetic variation within genotypes of meadow fescue (Festuca pratensis Huds)

Czech Journal of Genetics and Plant Breeding, 2010
The protein diversity of 45 genotypes (forty genotypes selected within two gene pools and five varieties) of meadow fescue was examined. Genetic variation was described using ISTA/UPOV methods for crop variety identification.
S. Stoyanova, B. Boller
doaj   +1 more source

Two-Dimensional Blue Native/SDS Polyacrylamide Gel Electrophoresis for Analysis of Brazilian Bothrops Snake Venoms

Toxins, 2022
Viperidae snakes are the most important agents of snakebites in Brazil. The protein composition of snake venoms has been frequently analyzed by means of electrophoretic techniques, but the interaction of proteins in venoms has barely been addressed.
Natacha Ferreira de Oliveira, Ana Teresa Azevedo Sachetto, Marcelo Larami Santoro   +2 more
doaj   +1 more source

Comparison of low molecular weight glutenin subunits identified by SDS-PAGE, 2-DE, MALDI-TOF-MS and PCR in common wheat [PDF]

, 2010
Low-molecular-weight glutenin subunits (LMW-GS) play a crucial role in determining end-use quality of common wheat by influencing the viscoelastic properties of dough.
Branlard, Gerard, Rogers, William John, Liu, Li, Ikeda, Tatsuya M, Wang Aili, Yoshida, H., Branlard, G., Xia, X., Kolman, Maía de Los Angeles, Roberto J Peña, Ma, Wujun, Ma, W., Rogers, William J, Wang, A., Lerner, S.E., Gerard Branlard, Zhonghu He, Lerner, Silvia E., He, Zhonghu, Yan, Y., Wang Linhai, Linhai Wang, Lerner Silvia E, Wang, Aili, He, Z., Silvia E Lerner, Rogers William J, Wujun Ma, Branlard, Gerard,, Peña, R., Kolman María A, Xia Xianchun, William J Rogers, Appels, R., Kolman, María A, Yoshida Hisashi, Kolman, M.A., Peña, Roberto J, Yoshida, Hisashi, Rogers, W.J., Liu, L., Lerner, Silvia E, Tatsuya M Ikeda, Ma Wujun, Branlard, Gerard, G., Wang, L., Yan, Yueming, Wang, Linhai, Appels, Rudi, Kolman, Maria de Los Angeles, Hisashi Yoshida, Appels Rudi, He Zhonghu, Peña Roberto J, Liu Li, Aili Wang, Ikeda Tatsuya M, María A Kolman, Peña, R.J., Li Liu, Yan Yueming, Xia, Xianchun, Ikeda, T.M., Yueming Yan, Rogers, John William, Xianchun Xia, Branlard Gerard, Rudi Appels, Ikeda, Tatsuya M., Peña, Roberto J.   +69 more
core   +1 more source

SDS-PAGE and Western blot analysis of fusion protein.

, 2021
(A)SDS-PAGE result of fusion protein in the process of purification (M, marker; lane1, loading solution; lane2, flow-through solution; lane3-4, 20 mM imidazole elution fraction; lane5, 50 mM imidazole elution fraction; lane6, 500 mM imidazole elution ...
Qiongqiong Bai (11285977), Jingpeng Zhang (9720374), Hai Jiang (445754), Han Li (10885), Jihong Shao (11285980), Mingjun Sun (532482), Dehui Yin (577156), Xiling Wu (454188)   +7 more
core   +1 more source

SDS-PAGE-Western blot of different HIV-1 Env strains bearing I559P alone or in combination with NFL.

, 2023
(A) VLPs expressing Q23-17 (Lanes 1–6), T250-4 (Lanes 7–12) and WITO.33 (Lanes 13–18), parent and mutant combinations were lysed and boiled in SDS/DTT, followed by digestion with endo H or PBS.
Tommy Tong (426980), Himanshi Chawla (10522421), Alessio D’Addabbo (11597230), Jiamin Xu (5613173), James M. Binley (8026892), Paola Ochoa (16793086), Max Crispin (346213), Albert Nguyen (2580886)   +7 more
core   +1 more source

SDS-PAGE and mass spectrometry analyses.

, 2022
Bacterial lysates (50 μg) of the original (OG) and mutant (MT) strains were subjected to an SDS-PAGE analysis, followed by CBB staining. A major band (arrowhead), which is clearly detected in MT, was identified as the TDE_1072 protein by mass ...
Mari Fujita (397912), Masahiro Iijima (5831621), Tadaharu Yokogawa (12936951), Keiji Nagano (136297), Hiroshi Miyakawa (4162141)   +4 more
core   +1 more source

Molluscan Shell Matrix Characterization by Preparative SDS-PAGE

The Scientific World Journal, 2003
The glycoproteinaceous constituents of molluscan shell matrices usually resist chromatographical fractionation. We describe a protocol that overcomes this difficulty and permits collection of a large amount of shell proteins for further in vitro ...
Frederic Marin
doaj   +1 more source

SDS-PAGE v1

, 2021
SDS-PAGE gels are used to visualize proteins. This protocol describes how to prepare all the buffers required for casting and running SDS-PAGE gels, as well as how to prepare whole cell samples.
Anna Bird, Chiara Gandini
openaire   +1 more source

A FAST AND SENSITIVE ZYMOGRAPHY METHOD OF PEROXIDASE ACTIVITY DETERMINATION USING SODIUM ACETATE BUFFER

Studia Universitatis Babes-Bolyai Chemia, 2019
By changing the buffer composition from phosphate to acetate (in both cases, the pH value was 6.0), the sensitivity of the estimation of peroxidase activity in polyacrylamide gels was improved from 4 µg/well to 0.4 µg/well.
Gheorghița MENGHIU, Amalia Nicoleta IANCU, Vasile OSTAFE   +2 more
doaj   +1 more source