Results 31 to 40 of about 684,938 (261)

Effects of Osmotic Stress Induced by Polyethylene Glycol (Peg) 6000 and Mannitol on Seed Germination and Seedling Growth of Durum Wheat

open access: yesJournal of Bioresource Management, 2021
Seed germination is generally the critical step in seed establishment and thus the determination of successful crop production. This study was focused at examination of the biochemical and germination parameters effected by low water potential which was ...
Ratiba Bousba   +4 more
doaj   +1 more source

An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane

open access: yesFEBS Letters, EarlyView.
Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa   +3 more
wiley   +1 more source

The ubiquitin ligase RNF115 is required for the clearance of damaged lysosomes

open access: yesFEBS Letters, EarlyView.
Upon lysosomal rupture, an E3 ubiquitin ligase RNF115 translocates from the cytosol to the damaged lysosomal membrane. Moreover, RNF115 depletion impairs the clearance of damaged lysosomes, identifying it as a key regulator of lysosomal quality control.
Sae Nakanaga   +3 more
wiley   +1 more source

SDS-PAGE v2

open access: yes, 2022
SDS-PAGE gels are used to visualize proteins. This protocol describes how to prepare all the buffers required for casting and running SDS-PAGE gels, as well as how to prepare whole cell samples.
openaire   +1 more source

Septin 9 PB domains coordinate centrosome positioning and microtubule acetylation to control epithelial polarity

open access: yesFEBS Letters, EarlyView.
Septin 9 polybasic domains couple phosphoinositide‐rich membrane binding to centrosome positioning, Golgi organization, and microtubule acetylation to control epithelial polarity. Their loss disrupts this axis, causing centrosome mispositioning, Golgi fragmentation, reduced microtubule acetylation, and polarity inversion via upregulation of the ...
Ting ting Cai   +4 more
wiley   +1 more source

SDS-PAGE v1

open access: yes, 2022
This protocol shows the steps carried out by team Tec-Chihuahua to perform SDS-PAGE
openaire   +1 more source

Degradation mechanism of the von Willebrand factor A2 domain by nattokinase

open access: yesFEBS Letters, EarlyView.
Nattokinase, a natto‐derived protease, exhibits potent antithrombotic effects. This study demonstrates that nattokinase directly cleaves the von Willebrand factor (vWF) A2 domain in vitro. Unlike the native regulator ADAMTS13, nattokinase degrades folded vWF independently of shear stress.
Ryuichi Hyakumoto   +3 more
wiley   +1 more source

Sugar Beet Leaf Culturable Endophytic Bacterial Composition from the Major Sugar Beet Growing Areas in the West of Iran [PDF]

open access: yesJournal of Genetic Resources, 2018
Endophyte bacteria refers to the bacteria that live within plants. Fresh leaves of 23 sugar beet (Beta vulgaris L.) plants were collected from the west of Iran.
Milad Aeini   +2 more
doaj   +1 more source

Modulation of Homer1 EVH1 domain internal dynamics by putative autism‐associated mutations

open access: yesFEBS Letters, EarlyView.
The putative autism‐associated M65I and S97L variants of the EVH1 domain of the postsynaptic scaffold protein Homer1 do not exhibit substantial changes in their overall structure or partner binding. Both of them, but especially the M65I variant, show altered internal dynamics relative to the wild‐type domain on the μs‐ms timescale, indicated by the ...
Fanni Farkas   +6 more
wiley   +1 more source

TUYỂN CHỌN GIỐNG KHOAI LANG HỒNG ĐÀO VÀ TÍM NHẬT THEO HƯỚNG NĂNG SUẤT CAO VÀ CHẤT LƯỢNG NGON BẰNG KỸ THUẬT ĐIỆN DI PROTEIN SDS-PAGE

open access: yesTạp chí Khoa học Đại học Cần Thơ, 2005
Fifty tubers of each variety (Hong Đao and Tim Nhat), totally 100 tubers, were analysed by protein SDS-PAGE and Lowry methods in order to screen and strengthen, these two varieties approaching to increasing higher protein content.
Châu Thị Anh Thy   +2 more
doaj  

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