Results 11 to 20 of about 4,922,198 (308)

Optimization of a TEEDA–based initiation system for SDS–PAGE [PDF]

open access: yesActa Biochimica Polonica
Polyacrylamide gel preparation for SDS–PAGE is conventionally initiated by the ammonium persulfate (APS)–TEMED system. Although effective, the relatively high volatility of TEMED may increase operator exposure during routine laboratory use. In this study,
Chaoyang Chen   +6 more
doaj   +2 more sources

SDS-PAGE to Immunoblot in One Hour [PDF]

open access: yesMethods Mol Biol, 2015
An ultra-rapid method for electrophoresing proteins on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, transfer of proteins to nitrocellulose membranes, and immunoblotting is described here. Electrophoresis of the autoantigens La and Ro60, as well as molecular weight standards on a 4-20 % gradient gel, was performed in about 10 min using ...
Biji T, Kurien   +3 more
openaire   +3 more sources

SDS-PAGE technique as biomarker for fish toxicological studies [PDF]

open access: yesToxicology Reports, 2018
Although many studies on the hematological and biochemical parameters in fishes have been done, still there are some shortage in the estimation and evaluation of the baseline’s values of marine and freshwater fishes.
Ola I. Muhammad   +3 more
doaj   +2 more sources

SDS-PAGE-Western blot of gp160ΔCT mutants.

open access: yes, 2023
VLPs expressing various JR-FL gp160ΔCT E168K+N189A mutant combinations were analyzed by reducing SDS-PAGE-Western blot. Duplicate blots were probed with A) anti-gp120, B) anti-gp41 or C) anti-gp120+gp41 MAb cocktails. Env species are indicated by colored
Tommy Tong (426980)   +7 more
core   +1 more source

The Carboxyl-Terminal Amino Acids Render Pro-Human LC3B Migration Similar to Lipidated LC3B in SDS-PAGE [PDF]

open access: yes, 2013
LC3 is widely used marker for macroautophagy assays. After translation pro-LC3 is processed by Atg4 to expose C-terminal glycine residue for downstream conjugation reactions to accomplish the conversion of LC3-I to LC3-II. SDS-PAGE based Western blot (Wb)
Wang, W   +22 more
core   +2 more sources

2D Diagonal Redox SDS-PAGE of Proteins

open access: yesBio-Protocol, 2013
2D diagonal redox SDS-PAGE of proteins is used to detect intramolecular or intermolecular disulfide bridges using Chlamydomonas in this example (Stroeher and Dietz, 2008; Schwarz et al., 2012).
Christian Schwarz, Joerg Nickelsen
doaj   +1 more source

HETEROGENEITY OF PROTEINS IN BIRDS’ EGG-WHITES

open access: yesBiotropia: The Southeast Asian Journal of Tropical Biology, 2019
Little is known about the comparative heterogeneity of protein compounds among a wide variety of birds’ egg white, particularly, an analysis of their detailed, in-parallel protein composition.
Hayder Obayes Hashim   +2 more
doaj   +1 more source

SDS platform: Use the SDS Mosaic widget

open access: yes, 2023
In this self-help guide, you will learn:what the SDS Mosaic widget does;how to use the code generator and Mosaic editor to add the widget to a webpage.LinksSDS service: https://www.sds.ox.ac.ukFeedSax example page: https://www.sds.ox.ac.uk/feeding-anglo ...
SDS Admin (10042286)
core   +1 more source

SDS-PAGE analysis of protein expression.

open access: yes, 2023
SDS-PAGE analysis of protein induction times (in hours indicated at the top of every gel) at two temperatures for all four Fab fragments. The target protein (histag-mAb-scFab c.a. 55 kDa) is boxed in red. (JPG)
Grant Frahm (14799697)   +12 more
core   +1 more source

SDS-PAGE v1 [PDF]

open access: yes, 2019
Protocol for SDS PAGE gel, buffers and Coomassie blue dye.
Anna Behle, Anika Wiegard
openaire   +3 more sources

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