Structure of the substrate-engaged SecA-SecY protein translocation machine [PDF]
Nature Communications, 2019 Proteins are translocated across membranes through the Sec61/SecY channel. Here, the authors present the structure of a translocating peptide chain trapped inside the SecA-SecY complex which suggests how peptides are actively moved through the channel.
Chengying Ma +7 more
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Two-way communication between SecY and SecA suggests a Brownian ratchet mechanism for protein translocation [PDF]
eLife, 2016 The essential process of protein secretion is achieved by the ubiquitous Sec machinery. In prokaryotes, the drive for translocation comes from ATP hydrolysis by the cytosolic motor-protein SecA, in concert with the proton motive force (PMF). However, the
William John Allen +7 more
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AFM observation of protein translocation mediated by one unit of SecYEG-SecA complex [PDF]
Nature CommunicationsProtein translocation across cellular membranes is an essential and nano-scale dynamic process. In the bacterial cytoplasmic membrane, the core proteins in this process are a membrane protein complex, SecYEG, corresponding to the eukaryotic Sec61 complex,
Yui Kanaoka +11 more
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A small molecule allosterically activates SecA dependent secretion [PDF]
Communications BiologyThe Sec pathway is an essential protein secretion route for all organisms. In bacteria, the SecA ATPase peripherally associates with the SecYEG channel to form the translocase that mediates preprotein export.
Haitham Sedky +8 more
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How SecB maintains clients in a translocation competent state [PDF]
Communications BiologyBacterial secretory proteins must remain soluble and non-folded until they reach the SecYEG translocase. Preprotein intrinsic features and chaperones can delay protein folding.
Guillaume Roussel +4 more
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Screening a library of temperature-sensitive mutants to identify secretion factors in Staphylococcus aureus [PDF]
Journal of BacteriologyProtein secretion is an essential cell process in bacteria, required for cell envelope biogenesis, export of virulence factors, and acquisition of nutrients, among other important functions. In the Sec secretion pathway, signal peptide-bearing precursors
Owen Leddy +6 more
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Microbial membrane transport genes in maize rhizosphere under fertilization – a preliminary study [PDF]
Scientific ReportsMicrobial DNA fragments isolated from maize rhizosphere soil under different soil fertilization treatments were sequenced, and the analysed genes contained membrane transport genes for the uptake and utilization of various substrates, nutrients ranging ...
Matthew Chekwube Enebe +1 more
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Thermodynamics of the ATPase Cycle of GlcV, the Nucleotide-Binding Domain of the Glucose ABC Transporter of Sulfolobus solfataricus [PDF]
, 2006 ATP-binding cassette transporters drive the transport of substrates across the membrane by the hydrolysis of ATP. They typically have a conserved domain structure with two membrane-spanning domains that form the transport channel and two cytosolic ...
Albers, Sonja-Verena, +5 more
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Signal recognition particle and SecA cooperate during export of secretory proteins with highly hydrophobic signal sequences. [PDF]
PLoS ONE, 2014 The Sec translocon of bacterial plasma membranes mediates the linear translocation of secretory proteins as well as the lateral integration of membrane proteins.
Yufan Zhou +2 more
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Protein secretion zones during overexpression of amylase within the Gram-positive cell wall
BMC Biology, 2023 Background Whereas the translocation of proteins across the cell membrane has been thoroughly investigated, it is still unclear how proteins cross the cell wall in Gram-positive bacteria, which are widely used for industrial applications. We have studied
Manuel Strach +4 more
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