Results 31 to 40 of about 34,551 (199)

Nucleotide sequence of the secA gene and secA(Ts) mutations preventing protein export in Escherichia coli [PDF]

Journal of Bacteriology, 1988
The DNA sequence of the secA gene, essential for protein export in Escherichia coli, was determined and found to encode a hydrophilic protein of 901 amino acid residues with a predicted molecular weight of 101,902, consistent with its previously determined size and subcellular location.
M G, Schmidt, E E, Rollo, J, Grodberg, D B, Oliver   +3 more
openaire   +2 more sources

Cloning and Expression Analysis of Phytoplasma Protein Translocation Genes

Molecular Plant-Microbe Interactions, 2001
Genes encoding SecA and SecY proteins, essential components of the Sec protein translocation system, were cloned from onion yellows phytoplasma, an unculturable plant pathogenic bacterium. The secA gene consists of 2,505 nucleotides encoding an 835 amino
Shigeyuki Kakizawa, Kenro Oshima, Tsutomu Kuboyama, Hisashi Nishigawa, Hee-young Jung, Toshimi Sawayanagi, Tsuneo Tsuchizaki, Shin-ichi Miyata, Masashi Ugaki, Shigetou Namba   +9 more
doaj   +1 more source

In Vitro Interaction of the Housekeeping SecA1 with the Accessory SecA2 Protein of Mycobacterium tuberculosis. [PDF]

PLoS ONE, 2015
The majority of proteins that are secreted across the bacterial cytoplasmic membrane leave the cell via the Sec pathway, which in its minimal form consists of the dimeric ATP-driven motor protein SecA that associates with the protein-conducting membrane ...
Irfan Prabudiansyah, Ilja Kusters, Arnold J M Driessen   +2 more
doaj   +1 more source

Mobility of the SecA 2-helix-finger is not essential for polypeptide translocation via the SecYEG complex [PDF]

, 2012
The bacterial ATPase SecA and protein channel complex SecYEG form the core of an essential protein translocation machinery. The nature of the conformational changes induced by each stage of the hydrolytic cycle of ATP and how they are coupled to protein ...
Alice Robson, Bauer, Berger, Bessonneau, Breyton, Brundage, Cannon, Dalal, Deville, Ding, Duong, Egea, Erlandson, Erlandson, Gelis, Gold, Gold, Gold, Hizlan, Hunt, Ian Collinson, Karamanou, Lill, Or, Osborne, Osborne, Park, Park, Piggot, Piggot, Richard B. Sessions, Robson, Robson, Robson, Sarah Whitehouse, Tam, Van den Berg, Vicki A.M. Gold, William J. Allen, Wolf, Woodbury, Zimmer, Zimmer   +42 more
core   +2 more sources

Multiple SecA protein isoforms in Escherichia coli [PDF]

Journal of Bacteriology, 1987
To define the anti-SecA-LacZ antiserum, immunoprecipitates produced with either whole anti-SecA-LacZ rabbit antiserum or affinity-purified antibodies were used to analyze nondenatured lysates of Escherichia coli. The antiserum contains antibodies that recognize different proteins.
openaire   +2 more sources

The C-terminal tail of the bacterial translocation ATPase SecA modulates its activity

eLife, 2019
In bacteria, the translocation of proteins across the cytoplasmic membrane by the Sec machinery requires the ATPase SecA. SecA binds ribosomes and recognises nascent substrate proteins, but the molecular mechanism of nascent substrate recognition is ...
Mohammed Jamshad, Timothy J Knowles, Scott A White, Douglas G Ward, Fiyaz Mohammed, Kazi Fahmida Rahman, Max Wynne, Gareth W Hughes, Günter Kramer, Bernd Bukau, Damon Huber   +10 more
doaj   +1 more source

Conformational Dynamics of the Plug Domain of the SecYEG Protein-conducting Channel [PDF]

, 2011
The central pore of the SecYEG preprotein-conducting channel is closed at the periplasmic face of the membrane by a plug domain. To study its conformational dynamics, the plug was labeled site-specifically with an environment-sensitive fluorophore.
Arkowitz, Baneyx, Becker, Bol, Bonardi, Bondar, Cabelli, De Keyzer, Driessen, du Plessis, Duong, Egea, Evans, Fekkes, Frauenfeld, Greenough, Gumbart, Hanahan, Harris, Joly, Junne, Klose, Kusters, Lakowicz, Li, Lin, Lycklama, Maillard, Manting, Ménétret, Nakatogawa, Osborne, Plath, Prinz, Ramachandran, Rutkowska, Schaffitzel, Smith, Tam, Tsukazaki, Van den Berg, van der Does, van der Sluis, Zhang, Zimmer   +44 more
core   +2 more sources

SecY and SecA interact to allow SecA insertion and protein translocation across the Escherichia coli plasma membrane [PDF]

The EMBO Journal, 1997
SecA, the preprotein-driving ATPase in Escherichia coli, was shown previously to insert deeply into the plasma membrane in the presence of ATP and a preprotein; this movement of SecA was proposed to be mechanistically coupled with preprotein translocation.
G, Matsumoto, T, Yoshihisa, K, Ito
openaire   +2 more sources

HDX-MS reveals nucleotide-dependent, anti-correlated opening and closure of SecA and SecY channels of the bacterial translocon

eLife, 2019
The bacterial Sec translocon is a multi-protein complex responsible for translocating diverse proteins across the plasma membrane. For post-translational protein translocation, the Sec-channel – SecYEG – associates with the motor protein SecA to mediate ...
Zainab Ahdash, Euan Pyle, William John Allen, Robin A Corey, Ian Collinson, Argyris Politis   +5 more
doaj   +1 more source

Functional characterization of the Staphylococcus carnosus SecA protein in Escherichia coli and Bacillus subtilis secA mutant strains [PDF]

FEMS Microbiology Letters, 1995
The Staphylococcus carnosus secA gene was cloned using the Bacillus subtilis secA gene as a probe. The S. carnosus secA encodes a polypeptide of 844 amino acid residues which is homologous to other known SecA proteins. The S. carnosus SecA functionally complemented the growth and secretion defects of a temperature-sensitive B.
M, Klein, J, Meens, R, Freudl
openaire   +2 more sources