Results 81 to 90 of about 195,148 (324)

Promiscuous stimulation of HSP70 ATPase activity by parasite‐derived J‐domains

FEBS Open Bio, EarlyView.
The malaria parasite Plasmodium falciparum exports three highly homologous yet functionally divergent J‐domain proteins into human erythrocytes. Here, we show that J‐domains isolated from all three proteins effectively stimulate the ATPase activity of both endogenous host and exported parasite HSP70 chaperones.
Julian Barth, Moritz Koch, Le‐Han Rössner, Johanna Eichhorn, Denys Pogoryelov, Matthias P. Mayer, Jude M. Przyborski   +6 more
wiley   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Second order analysis of state-constrained control-affine problems [PDF]

, 2016
International audienceIn this article we establish new second order necessary and suffi-cient optimality conditions for a class of control-affine problems with a scalar control and a scalar state constraint.
Aronna, Maria Soledad, Bonnans, J. Frederic, Goh, Bean San   +2 more
core   +2 more sources

SECOND-ORDER NECESSARY CONDITIONS FOR OPTIMAL IMPULSIVE CONTROL

IFAC Proceedings Volumes, 2005
Second-order conditions of optimality for an impulsive control problem are presented and derived from those for an appropriate abstract optimization problem with equality, inequality and set inclusion constraints. An important feature of these conditions is that they do not degenerate for abnormal control processes in spite of the absence of a priori ...
Arutyunov A., Pereira F.
openaire   +2 more sources

Chameleon sequences reveal structural effects in proteins representing micelle‐like distribution of hydrophobicity

FEBS Open Bio, EarlyView.
Amino acids sequence of two different proteins with the same sequence (chameleon sequence—black boxes) represent in 3D structure of the proteins different secondary structures: HHHH—helical and BBB—Beta‐structural. The chains folded in water environment adopt different III‐order structures in which the chameleon fragments appear to adopt similar status
Irena Roterman, Katarzyna Stapor, Leszek Konieczny, Krzysztof Gądek, Piotr Nowakowski   +4 more
wiley   +1 more source

Saddle Points of Partial Augmented Lagrangian Functions

Mathematical and Computational Applications
In this paper, we study a class of optimization problems with separable constraint structures, characterized by a combination of convex and nonconvex constraints.
Longfei Huang, Jingyong Tang, Yutian Wang, Jinchuan Zhou   +3 more
doaj   +1 more source

Second-order optimality conditions for bilevel programs [PDF]

, 2023
Xiang Liu, Mengwei Xu, Liwei Zhang
openalex   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Second order conditions of optimality for constrained optimization problems in finite dimensional spaces [PDF]

Conditions of optimality for constrained optimization ...
Masserli, E. J., Polak, E.
core   +1 more source