Results 91 to 100 of about 612,434 (304)

Clinical performance of the urine‐based TERT promoter AbsoluteQ Digital PCR for non‐invasive detection of bladder cancer

Molecular Oncology, EarlyView.
A urine‐based digital PCR assay targeting two hotspot TERT promoter variants detected bladder cancer with high sensitivity and no false positives in this case–control cohort. The streamlined AbsoluteQ workflow outperformed Sanger sequencing and supports non‐invasive molecular testing for bladder cancer detection.
Anna Nykel, Izabela Kubiak, Lena Rutkowska, Żaneta Kasprzyk, Łukasz Kępczyński, Michał Bednarek, Dariusz Sobieraj, Jacek Wilkosz, Piotr Kania, Adam Jędrzejczyk, Bogdan Kałużewski, Agnieszka Gach, Tadeusz Kałużewski   +12 more
wiley   +1 more source

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Assessing Differences in Groundwater Hydrology Dynamics Between In Situ Measurements and GRACE-Derived Estimates via Machine Learning: A Test Case of Consequences for Agroecological Relationships Within the Yazoo–Mississippi Delta (USA)

Hydrology
In situ groundwater monitoring is critical for irrigated agroecosystems and informs land cover changes. Yet, such data can pose management challenges and confound agroecological relationships.
Lucas J. Heintzman, Zahra Ghaffari, Abdel R. Awawdeh, Damien E. Barrett, Lance D. Yarbrough, Greg Easson, Matthew T. Moore, Martin A. Locke, Hakan I. Yasarer   +8 more
doaj   +1 more source

Dihydroorotate dehydrogenase (DHODH) regulates trophoblast syncytialization through organelle stress–induced cellular senescence

FEBS Open Bio, EarlyView.
The inhibition of mitochondrial dihydroorotate dehydrogenase (DHODH) impairs syncytialization and induces cellular senescence via mitochondrial and endoplasmic reticulum stress in human trophoblast stem cells, elevating sFlt1/PlGF levels, a hallmark of placental dysfunction in hypertensive disorders of pregnancy.
Kanoko Yoshida, Kazuya Kusama, Ayaka Horiuchi, Yu Kawaguchi, Atsuya Tsuru, Mikihiro Yoshie, Kazuhiro Tamura   +6 more
wiley   +1 more source

Soil of the lower valley of the Dragonja river (Slovenia)

Acta Agriculturae Slovenica, 2015
Soil of the lower valley of the river Dragonja developed under specific soil-forming factors. Soil development in the area was influenced by alluvial sediments originating from surrounding hills, mostly of flysch sequence rocks, as a parent material, Sub-
Tomaž PRUS, Nina ZUPANČIČ, Helena GRČMAN   +2 more
doaj   +1 more source

Meta‐analysis fails to show any correlation between protein abundance and ubiquitination changes

FEBS Open Bio, EarlyView.
We analyzed over 50 published proteomics datasets to explore the relationship between protein levels and ubiquitination changes across multiple experimental conditions and biological systems. Although ubiquitination is often associated with protein degradation, our analysis shows that changes in ubiquitination do not globally correlate with changes in ...
Nerea Osinalde, Unai Alduntzin, Gorka Prieto, Ugo Mayor   +3 more
wiley   +1 more source

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

FEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn, Barbara Seroka, Ryszard Lazny, Zenon Lotowski, Thore Rohwerder   +4 more
wiley   +1 more source

The Analysis of Sustainable Development Dam in Way Sekampung, Lampung

Jurnal Teknik Hidraulik, 2018
The availability of water resources provides many benefits for the welfare of all human being. The Decrease of the availability of water resources is caused by various reasons, including as environmental damage of catchment area, human activities, land ...
Mirza Nirwansyah, Cecep Kusmana, Eriyatno Eriyatno, M.Yanuar J. Purwanto   +3 more
doaj   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Controls on channel morphodynamics in the Yellow River Estuary: Insights from a multi-method assessment under an evolving multi-channel regime

International Journal of Sediment Research
The Yellow River Estuary (YRE) in China has transitioned to a complex multi-channel regime since 2013, making understanding terminal channel morphodynamics crucial for effective water and sediment management.
Yanjie Sun, Xiaolong Song, Haijue Xu, Yuchuan Bai   +3 more
doaj   +1 more source