Results 221 to 230 of about 372,053 (263)
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Improving the catalytic efficiency of Pseudomonas aeruginosa lipoxygenase by semi-rational design
Enzyme and Microbial Technology, 2023Lipoxygenase (LOX) catalyzes the peroxidation of unsaturated fatty acids to produce hydroperoxides, which had been widely used in food, medicine and chemical industries due to its decoloration of food and conversion of renewable oils. Thus, higher catalytic activity and stability is desired for low-cost and expanded industrial applications of LOX.
Cuiping, Pang +5 more
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Science of The Total Environment
Acetylcholinesterase (AChE) has emerged as a significant biological recognition element in the biosensor field, particularly for the detection of insecticides. Nevertheless, the weak thermostability of AChE restricts its utilization due to the complexities associated with production, storage, and application environments.
Shuoqi, Jiang +3 more
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Acetylcholinesterase (AChE) has emerged as a significant biological recognition element in the biosensor field, particularly for the detection of insecticides. Nevertheless, the weak thermostability of AChE restricts its utilization due to the complexities associated with production, storage, and application environments.
Shuoqi, Jiang +3 more
openaire +4 more sources
International Journal of Biological Macromolecules, 2020
Chitinase is a promising biocatalyst for chitin biotransformation in the field of recalcitrant biomass degradation. Excellent catalytic performance is conducive to its commercial utilization. In this work, sequence- and structure-based semi-rational design was performed to evolve the thermostability and activity of a previously identified chitinase ...
Pei, Xu +5 more
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Chitinase is a promising biocatalyst for chitin biotransformation in the field of recalcitrant biomass degradation. Excellent catalytic performance is conducive to its commercial utilization. In this work, sequence- and structure-based semi-rational design was performed to evolve the thermostability and activity of a previously identified chitinase ...
Pei, Xu +5 more
openaire +2 more sources
Applied Microbiology and Biotechnology, 2021
Xylanases have a broad range of applications in industrial biotechnologies, which require the enzymes to resist the high-temperature environments. The majority of xylanases have maximum activity at moderate temperatures, which limited their potential applications in industries.
Guangqi Li +7 more
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Xylanases have a broad range of applications in industrial biotechnologies, which require the enzymes to resist the high-temperature environments. The majority of xylanases have maximum activity at moderate temperatures, which limited their potential applications in industries.
Guangqi Li +7 more
openaire +2 more sources
International Journal of Biological Macromolecules, 2023
The application of (R)-ω-transaminases as biocatalysts for chiral amine synthesis has been hampered by inadequate stereoselectivity and narrow substrate spectrum. Herein, an effective evolution strategy for (R)-ω-transaminase designing for the asymmetric synthesis of sitagliptin intermediate is presented.
Fang-Ying, Zhu +7 more
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The application of (R)-ω-transaminases as biocatalysts for chiral amine synthesis has been hampered by inadequate stereoselectivity and narrow substrate spectrum. Herein, an effective evolution strategy for (R)-ω-transaminase designing for the asymmetric synthesis of sitagliptin intermediate is presented.
Fang-Ying, Zhu +7 more
openaire +2 more sources
Chemical Communications, 2016
Bad sugars in, good sugar out: an engineered sucrose phosphorylase for the production of kojibiose from sucrose and glucose.
Tom, Verhaeghe +6 more
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Bad sugars in, good sugar out: an engineered sucrose phosphorylase for the production of kojibiose from sucrose and glucose.
Tom, Verhaeghe +6 more
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Iterative Saturation Mutagenesis for Semi-rational Enzyme Design
2021In the quest to produce by directed evolution chemo-, stereo-, and regioselective enzymes as catalysts for the sustainable production of chiral compounds needed in modern society, focused saturation mutagenesis at sites surrounding the binding pocket has emerged as a particularly effective strategy.
Qu, G., Sun, Z., Reetz, M.
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Organic & Biomolecular Chemistry, 2018
Conduct structure-guided modification on the “hotspot” of glycosyltransferase UGTBL1 to significantly adjust its regioselectivity toward polydatin production.
Bo Fan +4 more
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Conduct structure-guided modification on the “hotspot” of glycosyltransferase UGTBL1 to significantly adjust its regioselectivity toward polydatin production.
Bo Fan +4 more
openaire +2 more sources
Semi-rational design of cellobiose dehydrogenase for increased stability in the presence of peroxide
Molecular Diversity, 2019Cellobiose dehydrogenase (CDH, EC 1.1.99.18) from white rot fungi Phanerochaete chrysosporium can be used for constructing biosensors and biofuel cells, for bleaching cotton in textile industry, and recently, the enzyme has found an important application in biomedicine as an antimicrobial and antibiofilm agent.
Ana Marija Balaž +6 more
openaire +3 more sources