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2010
Marker genes, such as gusA, lacZ, and gfp, have been applied comprehensively in biological studies. Directed in vitro evolution provides a powerful tool for modifying genes and for studying gene structure, expression, and function. Here, we describe a strategy for directed in vitro evolution of reporter genes based on semi-rational design and high ...
Ai-Sheng, Xiong +3 more
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Marker genes, such as gusA, lacZ, and gfp, have been applied comprehensively in biological studies. Directed in vitro evolution provides a powerful tool for modifying genes and for studying gene structure, expression, and function. Here, we describe a strategy for directed in vitro evolution of reporter genes based on semi-rational design and high ...
Ai-Sheng, Xiong +3 more
openaire +2 more sources
Semi‑rational design of cellobiose dehydrogenase for increased stability in the presence of peroxide
2020Cellobiose dehydrogenase (CDH, EC 1.1.99.18) from white rot fungi Phanerochaete chrysosporium can be used for constructing biosensors and biofuel cells, for bleaching cotton in textile industry, and recently, the enzyme has found an important application in biomedicine as an antimicrobial and antibiofilm agent.
Balaž, Ana Marija +6 more
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Enhancing thermostability of lysine hydroxylase via a semi-rational design
Process BiochemistryChengjuan Hu +6 more
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