Results 231 to 240 of about 372,053 (263)
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Semi-rational design of an imine reductase for asymmetric synthesis of alkylatedS-4-azepanamines
Organic & Biomolecular Chemistry, 2023An engineered imine reductase was used to catalyze the reductive amination ofN-Boc-4-oxo-azepane and diverse amines to synthesizeS-azepane-4-amines.
Fangfang Zhu +6 more
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[Improving the position specificity of Themomyces lanuginosus lipase based on semi-rational design].
Sheng wu gong cheng xue bao = Chinese journal of biotechnology, 2023Diacylglycerol (DAG) is an intermediate product in lipid metabolism and plays an important physiological role in human body. It is mainly prepared by hydrolyzing lipid with lipase. However, research on the detection method of 1, 2-diacylglycerol (1, 2-DAG) and 1, 3-diacylglycerol (1, 3-DAG) and catalytic specificity of lipase was not enough, which ...
Yadi, Ma +4 more
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International Journal of Biological Macromolecules
Phenylalanine ammonia-lyase (PAL) possesses significant potential in agriculture, industry, and the treatment of various diseases, including cancer. In particular, PAL derived from Anabaena variabilis (AvPAL) has been successfully utilized in clinical settings as an enzyme replacement therapy for phenylketonuria (PKU).
Xudong, Lü +7 more
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Phenylalanine ammonia-lyase (PAL) possesses significant potential in agriculture, industry, and the treatment of various diseases, including cancer. In particular, PAL derived from Anabaena variabilis (AvPAL) has been successfully utilized in clinical settings as an enzyme replacement therapy for phenylketonuria (PKU).
Xudong, Lü +7 more
openaire +2 more sources
bchm, 2007
Abstract Directed evolution in vitro is a powerful molecular tool for the creation of new biological phenotypes. It is unclear whether it is more efficient to mutate an enzyme randomly or to mutate just the active sites or key sites. In this study, the strategy of a semi-rational design of directed evolution combined with whole sequence ...
Ai-Sheng, Xiong +7 more
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Abstract Directed evolution in vitro is a powerful molecular tool for the creation of new biological phenotypes. It is unclear whether it is more efficient to mutate an enzyme randomly or to mutate just the active sites or key sites. In this study, the strategy of a semi-rational design of directed evolution combined with whole sequence ...
Ai-Sheng, Xiong +7 more
openaire +2 more sources
Enhanced thermostability of formate dehydrogenase via semi-rational design
Molecular Catalysis, 2022Xiaowen Wu +5 more
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Biotechnology Letters, 2020
To improve enzyme activity of Glucosamine-6-phosphate synthase (Glms) of Bacillus subtilis by site saturation mutagenesis at Leu593, Ala594, Lys595, Ser596 and Val597 based on computer-aided semi-rational design.The results indicated that L593S had the greatest effect on the activity of BsGlms and the enzyme activity increased from 5 to 48 U/mL.
Piwu Li +5 more
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To improve enzyme activity of Glucosamine-6-phosphate synthase (Glms) of Bacillus subtilis by site saturation mutagenesis at Leu593, Ala594, Lys595, Ser596 and Val597 based on computer-aided semi-rational design.The results indicated that L593S had the greatest effect on the activity of BsGlms and the enzyme activity increased from 5 to 48 U/mL.
Piwu Li +5 more
openaire +2 more sources
[Improving the activity of creatinase from Alcaligenes sp. KS-85 through semi-rational design].
Sheng wu gong cheng xue bao = Chinese journal of biotechnology, 2023Creatinine levels in biological fluids are important indicators for the clinical evaluation of renal function. Creatinase (CRE, EC3.5.3.3) is one of the key enzymes in the enzymatic measurement of creatinine concentration, and it is also the rate-limiting enzyme in the whole enzymatic cascade system.
Jiahao, Bian +2 more
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[Semi-rational design improves the catalytic activity of butyrylcholinesterase against ghrelin].
Sheng wu gong cheng xue bao = Chinese journal of biotechnologyGhrelin, a hormone mainly produced and released by the stomach, has numerous functions, including releasing growth hormones, regulating appetite, and processing sugar and lipids. Researchers have made great efforts to study the relationship between ghrelin and metabolic diseases.
Yingting, Cai +2 more
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Enzyme and Microbial Technology
The thermal instability of γ-glutamylmethylamide synthetase (GMAS) from Methylovorus mays has imposed limitations on its industrial applications, affecting both stability and activity at reaction temperatures. In this study, disulfide bridges were introduced through a combination of directed evolution and rational design to enhance GMAS stability ...
Chao, Fan +3 more
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The thermal instability of γ-glutamylmethylamide synthetase (GMAS) from Methylovorus mays has imposed limitations on its industrial applications, affecting both stability and activity at reaction temperatures. In this study, disulfide bridges were introduced through a combination of directed evolution and rational design to enhance GMAS stability ...
Chao, Fan +3 more
openaire +2 more sources
Enzyme and Microbial Technology
4-α-glucanotransferases (4GT) are valuable enzymatic tools with application in the food and pharmaceutical industries, particularly for producing thermoreversible starch gels. The screening of thermostable 4GT enzymes with high catalytic activity presents a significant challenge.
Qian Wang +7 more
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4-α-glucanotransferases (4GT) are valuable enzymatic tools with application in the food and pharmaceutical industries, particularly for producing thermoreversible starch gels. The screening of thermostable 4GT enzymes with high catalytic activity presents a significant challenge.
Qian Wang +7 more
openaire +2 more sources