Results 191 to 200 of about 112,157 (308)

Evaluation of statistical differential analysis methods for identification of senescent cells using single-cell transcriptomics. [PDF]

Cell Rep Methods
Li D, Liu P, Rahman I, Zand M, Pryhuber G, Dye T, Goniewicz M, Gurkar AU, Königshoff M, Eickelberg O, Mora A, Rojas M, Ma Q, Lugo-Martinez J, Bar-Joseph Z, Lanna S, Finkel T, Xie Z.   +17 more
europepmc   +1 more source

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Abrogation of aberrant glycolytic interactions eliminates senescent cells and alleviates aging-related dysfunctions. [PDF]

Signal Transduct Target Ther
Mikawa T, Kameda M, Ikari S, Shibata E, Liu S, Miyagawa S, Ono K, Ito T, Yoshizawa A, Sugimoto M, Shibuya S, Shimizu T, Almunia J, Ogiso N, Revêchon G, Palazzo A, Bernard D, Kanda H, Soga T, Takubo K, Morioka S, Sasaki J, Sasaki T, Itamoto A, Fujii T, Seno H, Inagaki N, Kondoh H.   +27 more
europepmc   +1 more source

Microglial dynamics and ferroptosis induction in human iPSC‐derived neuron–astrocyte–microglia tri‐cultures

FEBS Open Bio, EarlyView.
A tri‐culture of iPSC‐derived neurons, astrocytes, and microglia treated with ferroptosis inducers as an Induced ferroptosis model was characterized by scRNA‐seq, cell survival, and cytokine release assays. This analysis revealed diverse microglial transcriptomic changes, indicating that the system captures key aspects of the complex cellular ...
Hongmei Lisa Li, Hiroko Ohmiya, Sou Sakamoto, Masato Yugami, Akiko Oki, Makoto Furusawa, Yan Ling   +6 more
wiley   +1 more source

Increased substrate stiffness disrupts nuclear-cytoskeletal mechanical coupling in senescent cells. [PDF]

Mater Today Bio
Sohrabi Molina M, Brauer E, Günther R, Diederich S, Petersen A.   +4 more
europepmc   +1 more source

BMI‐1 modulation and trafficking during M phase in diffuse intrinsic pontine glioma

FEBS Open Bio, EarlyView.
The schematic illustrates BMI‐1 phosphorylation during M phase, which triggers its translocation from the nucleus to the cytoplasm. In cycling cells, BMI‐1 functions within the PRC1 complex to mediate H2A K119 monoubiquitination. Following PTC596‐induced M phase arrest, phosphorylated BMI‐1 dissociates from PRC1 and is exported to the cytoplasm via its
Banlanjo Umaru, Deepak Kumar Mishra, Shiva Senthil Kumar, Hao‐Han Pang, Brendan Devine, Komal Khan, Rachid Drissi   +6 more
wiley   +1 more source

Force-Dependent Presence of Senescent Cells Expressing Vascular Endothelial Growth Factor During Orthodontic Tooth Movement. [PDF]

Biology (Basel)
Morihana Y, Nakagawa M, Zhou Y, Morikuni H, Deng Z, Honda Y, Nishiura A.   +6 more
europepmc   +1 more source

The cooperative regulation of miR‐221 by APE1 and AUF1 impacts p27Kip1 defining a miR signature relevant for cervical cancer

FEBS Open Bio, EarlyView.
A regulatory axis involving APE1, AUF1, and miR‐221 is proposed. Pri‐miR‐221 is processed by DROSHA and DICER to generate mature miR‐221, which targets p27Kip1 mRNA. APE1 and AUF1 compete for pre‐miR‐221 binding. Reduced APE1/AUF1 levels impair miR‐221 biogenesis, decrease p27Kip1 mRNA degradation, and promote cell cycle progression, chemoresistance ...
Matilde Clarissa Malfatti, Nicolò Gualandi, Gianluca Tell, Giulia Antoniali   +3 more
wiley   +1 more source

Pharmacological clearance of senescent cells reduces inflammation, endothelial damage and cardiac fibrosis in HFpEF. [PDF]

Cardiovasc Res
Silva ED, Tomé I, Vasques-Nóvoa F, Conceição G, Silva A, Barros AS, Mendes-Ferreira P, Santos-Ribeiro D, Gonçalves A, Angélico-Gonçalves A, Adão R, Caetano C, Sousa D, Miranda-Silva D, Juni RP, Tchkonia T, Kirkland JL, Caramelo F, Gonçalves N, Sampaio-Pinto V, Ribeiro JS, Boon RA, Leite-Moreira A, Pitrez P, Pinto-do-Ó P, Santos SG, Falcão-Pires I, Ferreira L, Nascimento DS.   +28 more
europepmc   +1 more source

Dapagliflozin prevents methylglyoxal‐induced retinal cell death in ARPE‐19 cells

FEBS Open Bio, EarlyView.
Diabetic macular oedema is a diabetes complication of the eye, which may lead to permanent blindness. ARPE‐19 are human retinal cells used to study retinal diseases and potential therapeutics. Methylglyoxal is a compound increased in uncontrolled diabetes due to elevated blood glucose.
Naina Trivedi, Zainab Quraishi, Shima Khezri Azizi Far, Madaleine Ward, Aidan Conway, Reem Abdulredha, Eman Mshari, Yousif A. Shamsaldeen   +7 more
wiley   +1 more source