Results 101 to 110 of about 81,196 (301)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Summer soil moisture-convective precipitation coupling in Northern China at different timescales

open access: yesJournal of Hydrology: Regional Studies
Land-atmosphere coupling plays an important role in regional precipitation and climate change, but the coupling characteristics at different time scales remain controversial.
Hongwei Yang, Zesu Yang, Hang He
doaj   +1 more source

Sensible and latent heating of the atmosphere as inferred from DST-6 data [PDF]

open access: yes
The average distribution of convective latent heating, boundary layer sensible heat flux, and vertical velocity are determined for the winter 1976 DST period from GLAS model diagnostics. Key features are the regions of intense latent heating over Brazil,
Herman, G. F.   +2 more
core   +1 more source

Establishing an assay to evaluate d‐amino acid oxidase enzyme kinetics and inhibition using WST‐8 redox dye

open access: yesFEBS Open Bio, EarlyView.
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake   +4 more
wiley   +1 more source

Annual Observations of Climatic Impacts in the Songhua River Basin, China

open access: yesAdvances in Meteorology, 2015
Heat fluxes have been recognized as determinant regarding the river effect on microclimate. The relationship between rivers and the atmosphere is particularly significant for heat fluxes and varies with the meteorology and hydrology. In this study, based
Xiaocheng Song, Liu Jing
doaj   +1 more source

The International Urban Energy Balance Comparison Project: Initial Results from Phase 2. [PDF]

open access: yes, 2009
Many urban land surface schemes have been developed, incorporating different assumptions about the features of, and processes occurring at, the surface. Here, the first results from Phase 2 of an international comparison are presented.
Baik, J.J.   +5 more
core   +1 more source

Quantitative proteomic analysis reveals different characteristics of bladder cancer cells after exposure to bisphenol A

open access: yesFEBS Open Bio, EarlyView.
Bisphenol A (BPA), a common chemical in plastics, exerts dual effects on bladder cancer cells: low doses promote growth and migration, while high doses suppress growth and migration. Multi‐omics and bioinformatics reveal BPA acts via MAPK and inflammatory pathways.
Shaomin Niu   +10 more
wiley   +1 more source

Remote sensing of air-sea interactions [PDF]

open access: yes, 1984
A number of preliminary concepts for the measurement or inference of fluxes across the air-sea interface through remote sensing are proposed. All the methods are achievable from aircraft with state-of-the-art technology.
Atlas, D., Mollo-Christensen, E.
core   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

open access: yesFEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková   +3 more
wiley   +1 more source

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