Results 31 to 40 of about 12,439 (217)

Dynamic Characterization and Parametric Optimization of Secondary Cushioned Pump Valves in Drilling Systems: A 3D Transient Fluid–Structure Interaction Study

Applied Sciences
The dynamic response of pump valve motion directly influences the volumetric efficiency of drilling pumps and serves as a critical factor in performance enhancement.
Yi Wu, Yongjun Hou
doaj   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Numerical-simulation hybrid analysis of orthogonal cutting of Inconel 718

Journal of Mechanical Engineering and Sciences
Inconel 718 is widely employed in the aerospace and energy sectors due to its exceptional mechanical and thermal stability, yet it remains one of the most challenging materials to machine.
Md Adan Maqsood, Mohd Reyaz Ur Rahim, Prem Kumar Bharti, Danish Iqbal, Ayesha Tariq   +4 more
doaj   +1 more source

The Dynamic Characteristic of a Faulted Rotor System with Multi-Objective Optimization Designed SFD

Applied Sciences, 2019
As an important part of the turbomachinery, the rotor–bearing system has been upgraded to provide a high rotating speed in order to meet the demand of high power production.
Liang Ma, Jun Wang, Guichang Zhang
doaj   +1 more source

Establishing an assay to evaluate d‐amino acid oxidase enzyme kinetics and inhibition using WST‐8 redox dye

FEBS Open Bio, EarlyView.
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake, Yuki Enoki, Yuka Nakazawa, Kazuaki Taguchi, Kazuaki Matsumoto   +4 more
wiley   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Modification of the Tribomechanical Cutting Regime in Longitudinal-Torsional Ultrasonic Milling: From Adhesion to Controlled Fragmentation

Eng
Machining Nomex honeycomb structures presents a major challenge due to their thin-walled architecture, orthotropic behavior, and sensitivity to adhesion and delamination.
Oussama Beldi, Tarik Zarrouk, Ahmed Abbadi, Mohammed Nouari, Wenfeng Ding, Mohammed Abbadi, Jamal-Eddine Salhi, Mohammed Barboucha   +7 more
doaj   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Design and Parametric Sensitivity Analysis of a Steel-Concrete Hybrid Semi-Submersible Foundation Supporting a 15 MW Wind Turbine

Journal of Marine Science and Engineering
With the rapidly growing global demand for clean energy, offshore wind power has become an important renewable energy source. To clarify how the principal dimensions affect the performance of a 15 MW-class floating wind turbine platform in 100 m water ...
Wenwen Hu, Ling Wan, Shuai Li, Shuaibing Zhang, Yang Yang, Jungang Hao, Yajun Ren   +6 more
doaj   +1 more source