Results 61 to 70 of about 160,374 (321)
A clear separation of retinol, retinal, and retinoic acid has been achieved by liquid–gel partition chromatography on Sephadex LH-20 with solvent mixtures of chloroform, Skellysolve B, and methanol.
Y.L. Ito+3 more
doaj
This experiment was conducted in order to purify some active ingredients of 70% alcoholic extract of aniseed by adsorption chromatography on silica gel and gel filtration on sepharose CL-6B column, using phosphate buffer (0.1M, pH 7.0) as eluent, as ...
Anwar I. obead+2 more
doaj +1 more source
The NLS3 Motif in TPX2 Regulates Spindle Architecture in Xenopus Egg Extracts
ABSTRACT A bipolar spindle composed of microtubules and many associated proteins functions to segregate chromosomes during cell division in all eukaryotes, yet both spindle size and architecture vary dramatically across different species and cell types.
Guadalupe E. Pena+4 more
wiley +1 more source
Large-scale purification of factor VIII by affinity chromatography: optimization of process parameters [PDF]
The optimization of a new process for the extraction of human coagulation factor VIII (FVIII) from plasma with the tailor-made affinity matrix dimethylaminopropylcarbamylpentyl-Sepharose CL-4B (C3---C5 matrix) is described.
Bantjes, Adriaan+8 more
core +3 more sources
Impact of antibacterial drugs on human serum paraoxonase-1 (hPON1) activity: an in vitro study
Objective: To investigate the in vitro effects of the antibacterial drugs, meropenem trihydrate, piperacillin sodium, and cefoperazone sodium, on the activity of human serum paraoxonase (hPON1).
Hakan Söyüt+2 more
doaj +1 more source
Discovery of a Phenylalanine‐Derived Natural Compound as a Potential Dual Inhibitor of MDM2 and MDMX
P5, a novel phenylalanine‐derived compound isolated from Micromonospora sp. MS‐62, exhibits dual inhibition of MDM2 and MDMX. Molecular docking and SPR confirm its nanomolar affinity and sustained binding kinetics. In silico profiling reveals high GI absorption, BBB permeability, and drug‐likeness, highlighting its potential as a lead for p53‐targeted ...
Ja Young Cho+5 more
wiley +1 more source
Groups of rabbits were injected with either bovine serum albumin, sheep red cell stroma, or keyhole limpet hemocyanin to which 2,4-dinitrophenyl and/or p-azophenyl arsonate groups had been coupled. Groups of animals received either doubly coupled antigen
Amkraut, Alfred A.+2 more
core
Trypsin inhibitor from starchy endosperm of rye seeds
A method for the preparation of a trypsin inhibitor from starchy endosperm of rye seeds is described. Isolation and purification comprised the following steps: salting out of the water-soluble proteins at pH 4.5 with ammonium sulphate (0.4 saturation ...
A. Pawłowski
doaj +1 more source
This study demonstrates that hypoacylated, rough‐type lipopolysaccharides (LPS) derived from Akkermansia muciniphila strain HW07 (ALPS) alleviates diet‐induced obesity. ALPS acts as a weak TLR4 agonist and significantly reduces weight gain, improves metabolic parameters, and restores gut barrier integrity in obese mice.
Li Sun+14 more
wiley +1 more source
Salix daphnoides: A Screening for Oligomeric and Polymeric Proanthocyanidins
In the present study, a qualitative analysis of proanthocyanidins (PAs) from an aqueous-methanolic extract of Salix daphnoides VILL. bark is described. Procyanidin B1 (1), B2 (2), B3 (3), B4 (4), C1 (5), epicatechin-(4β→8)-epicatechin-(4β→8)-catechin (6)
Stefan Wiesneth+2 more
doaj +1 more source