Results 161 to 170 of about 240,931 (342)

Purification, Fluorescent Labeling, and Detyrosination of Mammalian Cell Tubulin for Biochemical Assays

open access: yesCytoskeleton, EarlyView.
ABSTRACT Microtubules play essential roles in numerous cellular processes. All microtubules are built from the protein tubulin, yet individual microtubules can differ spatially and temporally due to their tubulin isotype composition and post‐translational modifications (PTMs).
Ezekiel C. Thomas   +5 more
wiley   +1 more source

Blueberry exosome‐like nanoparticles extraction guidelines: Comparative methods and standardized operations

open access: yesFood Biomacromolecules, EarlyView.
Abstract Blueberry‐derived exosome‐like nanoparticles (BENs) hold significant potential for functional foods and biomedical applications. Efficient extraction is crucial for exploiting their benefits. This guideline compares four principal methods based on key operational and performance parameters.
Si‐Yi Li   +3 more
wiley   +1 more source

NOTIZEN: Gewinnung von Cobalamin-Coenzym-Sepharose / Preparation of Cobalamin Coenzyme-sepharose

open access: yesZeitschrift für Naturforschung C, 1973
Margarethe Schlecht, Otto Müller
openaire   +1 more source

Using generalized quantitative structure–property relationship (QSPR) models to predict host cell protein retention in ion‐exchange chromatography

open access: yesJournal of Chemical Technology &Biotechnology, EarlyView.
Abstract BACKGROUND Selecting an optimal chromatography resin during biopharmaceutical downstream process development is a great challenge. This is especially the case for recombinant subunit vaccines, where product properties vary greatly and recovery often involves cell lysis, which yields a complex mixture of different host cell materials. Host cell
Tim Neijenhuis   +4 more
wiley   +1 more source

HOXA5 Suppresses NEK7‐Mediated Alveolar Epithelial Pyroptosis in Acute Lung Injury by Transcriptionally Inhibiting KAT2A

open access: yesThe Kaohsiung Journal of Medical Sciences, EarlyView.
ABSTRACT Alveolar epithelial cell pyroptosis exacerbates inflammation and tissue damage by releasing inflammatory mediators, thereby promoting the development of acute lung injury (ALI). However, the fundamental mechanism underlying alveolar epithelial cell pyroptosis in ALI has not yet been elucidated. Lipopolysaccharide (LPS) was used to simulate ALI
Lei Wang   +5 more
wiley   +1 more source

Purification and Properties of Extracellular Endoinulinase from Aspergillus niger 20 OSM

open access: yesFood Technology and Biotechnology, 2006
Extracellular inulinase (E.C. 3.2.1.7) produced by Aspergillus niger 20 OSM culture in a 2-litre fermentor was isolated and purified by ion exchange chromatography on DEAE Sepharose, hydrophobic interaction on phenyl Sepharose, chromatofocusing on PBE-94,
Marcin Skowronek, Jan Fiedurek
doaj  

USP54 Promotes Ferroptosis in Non‐Small Cell Lung Cancer by Mediating FOXA2 Deubiquitination and Enhancing ACSL4 Transcription

open access: yesThe Kaohsiung Journal of Medical Sciences, EarlyView.
ABSTRACT Non‐small cell lung cancer (NSCLC) is the most common type of lung cancer, with a 5‐year survival rate of less than 20% and a high risk of recurrence despite advances in treatment. This study aimed to identify new therapeutic targets to increase the effectiveness of NSCLC treatments.
Rui‐Shi Wei   +5 more
wiley   +1 more source

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