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Nanopore RNA Sequencing Analysis

2021
The recent advent of Nanopore sequencing allows for the sequencing of full-length RNA or cDNA molecules. This new type of data introduces new challenges from the computational point of view, and requires new software as well as dedicated analysis pipelines.
Tommaso, Leonardi, Adrien, Leger
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RNA Sequencing Analysis of Saliva exRNA

2022
Next-generation sequencing (NGS) methodologies are rapidly developing. However, RNA Sequencing of saliva is challenging due to low abundance and integrity of extracellular RNA, as well as large amounts of bacterial RNAs that may be encountered in saliva. In addition, the literature about human salivary extracellular RNA is very scarce.
Karolina Elżbieta, Kaczor-Urbanowicz   +1 more
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Identification and Analysis of Palindromes for RNA Sequences

2016 IEEE 16th International Conference on Bioinformatics and Bioengineering (BIBE), 2016
A palindrome is a string of the form S = A1 A2 or S = A1 aA2, where A1 and A2 are substrings of S and the reverse of A2 exactly matches with A1. DNA palindromes show direct influence on tumerogenesis due to the formation of DNA palindromes at microRNA genes, which involve in tumor development. Furthermore, RNA palindromes play a crucial role in genomic
Chien-Hung Huang   +6 more
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Alternative expression analysis by RNA sequencing

Nature Methods, 2010
In alternative expression analysis by sequencing (ALEXA-seq), we developed a method to analyze massively parallel RNA sequence data to catalog transcripts and assess differential and alternative expression of known and predicted mRNA isoforms in cells and tissues.
Malachi, Griffith   +25 more
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RNA SEQUENCE ANALYSIS

Annual Review of Biochemistry, 1970
FRACTIONATION AND PURIFICATION OF RNA 228 Transfer RNA 228 Ribosomal RNA ... . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 230 Viral RNA 230 SEPARATION AND ISOLATION OF POLYNUCLEOTIDES 231 Physical methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
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RNA sequencing and Prediction Tools for Circular RNAs Analysis

2018
Circular RNAs (circRNAs) are noncoding and single-stranded RNA transcripts able to form covalently circular-closed structures. They are generated through alternative splicing events and widely expressed from human to viruses. CircRNAs have been appointed as potential regulators of microRNAs (miRNAs), RNA-binding proteins (RPBs), and lineal protein ...
Elena, López-Jiménez   +2 more
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In Silico Analysis of Micro-RNA Sequencing Data

2021
High-throughput sequencing for micro-RNAs (miRNAs) to obtain expression estimates is a central method of molecular biology. Surprisingly, there are a number of different approaches to converting sequencing output into micro-RNA counts. Each has their own strengths and biases that impact on the final data that can be obtained from a sequencing run. This
Ernesto, Aparicio-Puerta   +3 more
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Sequence and Structure Analysis of Noncoding RNAs

2009
Noncoding RNAs (ncRNAs) are increasingly recognized as important functional molecules in the cell. Here we give a short overview of fundamental computational techniques to analyze ncRNAs that can help us better understand their function. Topics covered include prediction of secondary structure from the primary sequence, prediction of consensus ...
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Analysis of Pteridium ribosomal RNA sequences by rapid direct sequencing

Plant Molecular Biology, 1991
A total of 864 bases from 5 regions interspersed in the 18S and 26S rRNA molecules from various clones of Pteridium covering the general geographical distribution of the genus was analysed using a rapid rRNA sequencing technique. No base difference has been detected amongst the three major lineages, two of which apparently separated before the breakup ...
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Differential analysis of rna methylation sequencing data

2013 IEEE Global Conference on Signal and Information Processing, 2013
Recently, a new technique, combining Methylated RNA Immunoprecipatation with RNA sequencing (MeRIP-Seq), was developed and applied to survey the global mRNA N6-methyladenosine (m6A) methylation in mammalian cells. MeRIP-Seq has the potential to survey, for the first time, the transcriptome-wide distribution of different types of post-transcriptional ...
Xiaodong Cui   +4 more
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