Results 81 to 90 of about 775,502 (352)

Molecular cloning and characterization of B-cadherin, a novel chick cadherin. [PDF]

, 1991
Calcium-dependent cell-cell adhesion is mediated in large part by a set of homologous integral membrane glycoproteins termed cadherins. In this report, antibodies to conserved domains in previously described cadherins have been used to isolate cDNAs ...
Napolitano, EW, Reichardt, LF, Venstrom, K, Wheeler, EF   +3 more
core   +2 more sources

Correlation of the differential expression of PIK3R1 and its spliced variant, p55α, in pan‐cancer

Molecular Oncology, EarlyView.
PIK3R1 undergoes alternative splicing to generate the isoforms, p85α and p55α. By combining large patient datasets with laboratory experiments, we show that PIK3R1 spliced variants shape cancer behavior. While tumors lose the protective p85α isoform, p55α is overexpressed, changes linked to poorer survival and more pronounced in African American ...
Ishita Gupta, Yang Song, Madeleine Ndahayo, Anirudh Saxena, Theresa Guo, Dylan Z. Kelley, Jessica Gore, Andrew Hennigan, Alexa Anderson, John C. Papadimitriou, Daria A. Gaykalova   +10 more
wiley   +1 more source

Cloning and function prediction of full-length cDNA for cathepsin E derived from medaka (Oryzias latipes)

浙江大学学报. 农业与生命科学版, 2017
Cathepsin is a kind of protease that mainly exists in intracellular lysosome. Under the weak acid condition, cathepsin can be activated and acts as hydrolysate protein.
WANG Jiaqing, DONG Huiming, LI Zhengang, LI Shaoming, WANG Ruonan, FU Yujie   +5 more
doaj   +1 more source

Genomic organization of nucleolin gene in carp fish: Evidence for several genes [PDF]

, 2006
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000200017&lng=es&nrm=isoThe protein nucleolin, functionally involved in the main steps of ribosome biogenesis, is codified by a single copy gene in mammals.
Alvarez, Marco, Molina, Alfredo, Navarro, Cristina, Quezada, Claudia, San Martín, Rody, Vera, Ines   +5 more
core   +2 more sources

Rethinking plastic waste: innovations in enzymatic breakdown of oil‐based polyesters and bioplastics

FEBS Open Bio, EarlyView.
Plastic pollution remains a critical environmental challenge, and current mechanical and chemical recycling methods are insufficient to achieve a fully circular economy. This review highlights recent breakthroughs in the enzymatic depolymerization of both oil‐derived polyesters and bioplastics, including high‐throughput protein engineering, de novo ...
Elena Rosini, Nicolò Antonelli, Gianluca Molla   +2 more
wiley   +1 more source

Identification of T-cell receptor a-chain genes in the chicken [PDF]

, 1994
T-cell receptor (TCR) -chain (TCR) and ß-chain (TCRß) genes are well characterized in mammals, while only TCRß genes have been identified in other vertebrates. To identify avian TCR genes, we used monoclonal anti-CD3 antibodies to isolate chicken TCR for
Aebersold, Ruedi, Chen, Chen-Lo H., Cooper, Max D., Göbel, Thomas W. F., Hood, Leroy, Kubota, Takayuki, Kuo, Chia-Lam, Lahti, Jill   +7 more
core  

Homologous expression and purification of human HAX‐1 for structural studies

FEBS Open Bio, EarlyView.
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley   +1 more source

Molecular characterization of MHC-DRB cDNA in water buffalo (Bubalus bubalis)

Genetics and Molecular Biology, 2012
In the present study, water buffalo MHC (Bubu)-DRB cDNA was cloned and characterized. The 1022 base long-amplified cDNA product encompassed a single open reading frame of 801 bases that coded for 266 amino acids.
Soumen Naskar, Sitangsu M. Deb, Saket K. Niranjan, Subodh Kumar, Deepak Sharma, Durgam Sakaram, Arjava Sharma   +6 more
doaj  

SmallTalk: a novel small‐sized fusion tag for peptide expression and purification

FEBS Open Bio, EarlyView.
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq, Nestor G. Casillas‐Vega, Alma Gomez‐Loredo, Xristo Zarate   +3 more
wiley   +1 more source

Genome-wide association study of rubella virus vaccine strain BRD-Ⅱ

Shanghai yufang yixue
ObjectiveTo perform a genome-wide association study of rubella virus vaccine strain BRD-Ⅱ, so as to fully grasp the sequence characteristics of this genome.MethodsSecond-generation sequencing method was used to conduct the whole-genome sequencing on the ...
XU Yingmei, ZHU Yongqiang, ZHOU Xin, LIU Zhaoyang, MA Leijun, CHEN Zhewen, ZHAO Yueye, ZHU Tiaoxia   +7 more
doaj   +1 more source