Results 201 to 210 of about 3,116,462 (307)

Differences in anti-amyloid therapy eligibility assessment based on use of the SWI versus GRE sequence. [PDF]

Alzheimers Dement (Amst)
Bermudez C, Pillai JJ, Burkett BJ, McDonald JS, Johnson DR, Wiste HJ, Weigand SD, Jack CR, Vemuri P, Petersen RC, Graff-Radford J, Ramanan VK, Cogswell PM.   +12 more
europepmc   +1 more source

USP7 inhibits FOXO1 activity in chronic lymphocytic leukaemia cells enhancing cell survival

Molecular Oncology, EarlyView.
CLL cells rely on BCR‐mediated signalling for survival and proliferation in part through suppression of FOXO1. FOXO1 activity is regulated by a plethora of post‐translational modifications including phosphorylation and ubiquitination. We demonstrated that dual inhibition of BCR signalling and the deubiquitinase USP7 activates FOXO1, modulating its ...
Hassan N.B. Almuhanna, Jodie Hay, Michael W. Moles, Lucy Clarke, Jennifer Cassels, Alison Sefcick, Alison M. McCaig, Heather G. Jørgensen, Alison M. Michie   +8 more
wiley   +1 more source

Nomenclature, classification, and in-silico structural analysis of MSP1a variants of Anaplasma marginale. [PDF]

PLoS One
Siddiki AZ, Humaira N, Hossain NS, Akbar YB, Palit P, Phwe PE, Eva RA, Alshaar H, Tia MI, Tia MI, Marma K, Akter B, Sultana S, Alam S.   +13 more
europepmc   +1 more source

The impact of leukocyte‐associated RNA retention on platelet RNA‐based cancer detection

Molecular Oncology, EarlyView.
Platelets carry RNA signals that may help detect cancer, but platelet samples can also retain RNA from white blood cells. We found that this retained white blood cell signal did not significantly affect machine learning cancer classification, suggesting that the cancer‐related RNA signal in platelets is largely independent of leukocyte‐associated RNA ...
Michał Sieczczyński, Krzysztof Pastuszak, Anna Samelak‐Czajka, Zuzanna Cemka, Paulina Jackowiak, Tomasz Stefaniak, Sylwia Łapińska‐Szumczyk, Jacek Jassem, Anna J. Żaczek, Matthew T. Rondina, Myron G. Best, Sjors G. J. G. In 't Veld, Anna Supernat   +12 more
wiley   +1 more source

Controlling the controls: On the importance of validated negative control ASOs. [PDF]

Mol Ther Nucleic Acids
Farkas J, Denti MA.
europepmc   +1 more source

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

<i>Cyclovirus</i>-Related DNA in Bovine Feces from Northern Portugal: Molecular Detection and Phylogenetic Characterization. [PDF]

Microorganisms
Figueiredo RJ, Gomes-Gonçalves S, Moreira G, Huettich K, Klaus S, Corley M, Prusa K, Alcobia BO, Mesquita JR.   +8 more
europepmc   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

High Conservation of the Human Metapneumovirus Fusion Protein in Contemporary Global Sequences Supports Its Continued Relevance for Therapeutic and Prophylactic Consideration. [PDF]

Microorganisms
Gopalakrishnan V, Schaefer E, Patterson J, Brady T, Francica JR, Kushnir AS, Podlaha O, Streicher K, Cohen TS, Aksyuk AA, Tuffy KM.   +10 more
europepmc   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source