Results 121 to 130 of about 910,209 (262)

Moving towards third-generation sequencing technologies

, 2012
Within just the past few years, next-generation sequencing (NGS) platforms have reduced the cost of DNA sequencing by several orders of magnitude. With these lower costs, DNA sequencing is increasingly powerful as a single-data format onto which a broad ...
Janitz, Michael, Janitz, Caroline (R17566), Karolina Janitz, Michal Janitz   +3 more
core   +1 more source

Polymorphism discovery and allele frequency estimation using high-throughput DNA sequencing of target-enriched pooled DNA samples. [PDF]

, 2012
peer-reviewedBackground: The central role of the somatotrophic axis in animal post-natal growth, development and fertility is well established. Therefore, the identification of genetic variants affecting quantitative traits within this axis is an ...
Magee, David A, Mullen Michael P, Creevey Christopher J, Park, Stephen D, Killeen Aideen P, Donagh P Berry, Killeen, Aideen P, Park Stephen D, Howard, Dawn J, McGettigan, Paul A., Christopher J Creevey, Creevey, Christopher J.; id_orcid, Matt C Lucy, Michael P Mullen, Waters, Sinead M., Howard Dawn J, David A Magee, Howard, Dawn J., Lucy Matt C, Mullen, Michael P, Berry Donagh P, Berry, Donagh P., McCabe, Matt S., Park, Stephen D., Magee, David A., McCabe Matt S, Dawn J Howard, Creevey, Christopher J., Matt S McCabe, Lucy, Matt C., Waters, Sinead M, McCabe, Matt S, Killeen, Aideen P., Lucy, Matt C, Magee David A, McGettigan Paul A, Paul A McGettigan, Waters Sinead M, MacHugh, David E, MacHugh David E, David E MacHugh, Aideen P Killeen, MacHugh, David E., Park, Stephen D. E., McGettigan, Paul A, McGettigan, Paul, Mullen, Michael P., McCabe, Matthew, Creevey, Christopher J, Berry, Donagh P, Sinead M Waters, Stephen D Park, Berry, Donagh   +52 more
core   +1 more source

Structure‐forward targeting of claudins with synthetic binders

FEBS Letters, EarlyView.
Claudins form the paracellular barriers between epithelial and endothelial tissues at tight junctions and are targets for molecular binders with the goal of modulating barrier permeability. Claudin‐binding molecules are relevant in drug delivery or in altering claudin interactions with disease‐causing proteins.
Alex J. Vecchio
wiley   +1 more source

Genetic sex validation for sample tracking in next-generation sequencing clinical testing

BMC Research Notes
Objective Data from DNA genotyping via a 96-SNP panel in a study of 25,015 clinical samples were utilized for quality control and tracking of sample identity in a clinical sequencing network.
Jianhong Hu, Viktoriya Korchina, Hana Zouk, Maegan V. Harden, David Murdock, Alyssa Macbeth, Steven M. Harrison, Niall Lennon, Christie Kovar, Adithya Balasubramanian, Lan Zhang, Gauthami Chandanavelli, Divya Pasham, Robb Rowley, Ken Wiley, Maureen E. Smith, Adam Gordon, Gail P. Jarvik, Patrick Sleiman, Melissa A. Kelly, Harris T. Bland, Mullai Murugan, Eric Venner, Eric Boerwinkle, the eMERGE III consortium, Cynthia Prows, Lisa Mahanta, Heidi L. Rehm, Richard A. Gibbs, Donna M. Muzny   +29 more
doaj   +1 more source

GRIPT: a novel case-control analysis method for Mendelian disease gene discovery

Genome Biology, 2018
Despite rapid progress of next-generation sequencing (NGS) technologies, the disease-causing genes underpinning about half of all Mendelian diseases remain elusive.
Jun Wang, Li Zhao, Xia Wang, Yong Chen, Mingchu Xu, Zachry T. Soens, Zhongqi Ge, Peter Ronghan Wang, Fei Wang, Rui Chen   +9 more
doaj   +1 more source

A ripple-spreading genetic algorithm for the aircraft sequencing problem [PDF]

, 2011
When genetic algorithms (GAs) are applied to combinatorial problems, permutation representations are usually adopted. Asa result, suchGAsare often confronted with feasibility and memory-efficiency problems.
Di Paolo, Ezequiel A., Hu, Xiao-Bing, Ezequiel A. Di Paolo, Xiao-bing Hu   +3 more
core   +1 more source

Peripheral lysosomes recruit PLEKHG3 to focal adhesions and restrain protrusion dynamics

FEBS Letters, EarlyView.
Proximity‐dependent labeling at the LAMTOR complex revealed the Rho GEF PLEKHG3 as a lysosome‐proximal protein directing the study toward the influence of lysosome positioning on actin dynamics and cell motility. We show that PLEKHG3 colocalizes with lysosomes at focal adhesion sites and observe that forced peripheral dispersion of lysosomes hinders ...
Rainer Ettelt, Ana‐Maria Sandru, Georg Vucak, Sebastian Didusch, Biljana Riemelmoser, Karin Ehrenreiter, Markus Hartl, Lukas A. Huber, Manuela Baccarini   +8 more
wiley   +1 more source

Engineering peptides into antibodies—opportunities and strategies for therapeutic innovation

FEBS Letters, EarlyView.
Peptides and antibodies occupy complementary therapeutic niches. Peptides recognize difficult targets in a compact format, while antibodies add specificity, long half‐life, and effector functions. This review examines strategies that merge both modalities—peptide grafting into loops, terminal and Fc fusions, and bioconjugation—highlighting how ...
Jinling Wang, Ruslana Romaniuk, Hristo L. Svilenov   +2 more
wiley   +1 more source

The sequencing of agricultural market reforms in Malawi [PDF]

The paper analyzes the welfare impacts of alternative sequencing scenarios of agricultural market reforms in Malawi using a profit maximization approach.
Kumaresan, Govindan, Kherallah, Mylène
core  

Liver organoids: modelling complexity in homeostasis and disease

FEBS Letters, EarlyView.
Studying liver in vitro has been challenging because simple 2D cell cultures fail to capture liver's cellular and architectural complexity. To bridge this gap, scientists increasingly use organoids, 3D liver models which better mimic liver composition and function. This review examines recent advances in liver organoid complexity and realism, discusses
Anna M. Dowbaj, Meritxell Huch
wiley   +1 more source