Results 101 to 110 of about 11,269 (219)

Cysteine Thioaldehydes: Photolytic Generation, Reactivity, and Biological Implications

Angewandte Chemie, EarlyView.
Photolysis of cysteine phenacylsulfides bearing non‐conjugating electron‐withdrawing substituents leads to high conversions into cysteine thioaldehydes through a Norrish type‐II pathway. This methodology enabled the study of the aqueous reactivity of these important biosynthetic intermediates, which, depending on peptide sequence, pH and buffer ...
Ardra Karthika, Matthew Murray, Guillermo Palop, Lee‐Ann J. Keane, Patrick McArdle, Nicholas D. J. Yates, Christopher D. Spicer, Alison Parkin, Martin A. Fascione, Eddie L. Myers   +9 more
wiley   +2 more sources

Protein Structuromics Reveals a Loop‐Controlled Half‐Open Active Pocket Conformation Throughout Fe(II)/α‐ketoglutarate‐Dependent Dioxygenase Catalytic Cycle

Advanced Science, EarlyView.
This study reveals a conserved half‐open active pocket in Fe(II)/α‐ketoglutarate‐dependent dioxygenases through protein structuromics, distinguished by structural features comprising highly flexible loop elements. Enhanced sampling simulations‐guided mutagenesis experiments elucidated how this flexible loop regulates pocket open/closed conformations ...
Lunjie Wu, Huan Liu, Songyin Zhao, Lei Qin, Jiarui Li, Jun Wang, Zixuan Dai, Jie Gu, Yan Xu, Feiran Li, Yao Nie   +10 more
wiley   +1 more source

A General Enzymatic Strategy for Site‐Specific Incorporation of Modified Genetic Building Blocks Into DNA

Advanced Science, EarlyView.
Eco‐friendly enzymatic synthesis of high‐fidelity, site‐specifically modified DNA using 3′‐blocked nucleotides for full synthetic control and next‐generation sequencing compatibility. ABSTRACT Methods for site‐specific incorporation of modified DNA building blocks remain limited.
Raveena Raveena, Bhavana Ramadas, Sidney Becker   +2 more
wiley   +1 more source

De Novo Discovery of Nonstandard Thioisoindole‐Bridged Bicyclic Peptides Targeting Traf2‐ and NCK‐Interacting Kinase

Angewandte Chemie, EarlyView.
An optimized strategy for the ribosomal synthesis of thioisoindole‐bridged bicyclic (TiB) peptides is incorporated into the RaPID platform, enabling high‐throughput discovery from topologically defined TiB libraries. Proof‐of‐concept selection against TNIK identifies nanomolar‐affinity ligands with inhibitory activity, while x‐ray crystallography ...
Yue Zhang, Alexander A. Vinogradov, Keisuke Hamada, Yin Sun, Toru Sengoku, Hiroaki Suga   +5 more
wiley   +2 more sources

Appraising protein conformational changes by resampling time-resolved serial x-ray crystallography data

Structural Dynamics
With the development of serial crystallography at both x-ray free electron laser and synchrotron radiation sources, time-resolved x-ray crystallography is increasingly being applied to study conformational changes in macromolecules.
Adams Vallejos, Gergely Katona, Richard Neutze   +2 more
doaj   +1 more source

In Situ Reconstructed Crystalline–Amorphous CuNi Nanotubes Unifying Activity and Stability for Oxygen Evolution

Advanced Science, EarlyView.
Controlled electrochemical reconstruction creates a crystalline–amorphous CuNi nanotube that unifies catalytic activity and durability for alkaline oxygen evolution. The conductive metallic core and active amorphous oxide shell work synergistically to deliver low overpotential and exceptional stability exceeding 1000 h, offering a new strategy for ...
Shi‐Yu Zhu, Han Gao, Meng Li, Ling‐Rui Wang, Tian‐Yu Xia, Wei‐Di Liu, Xiao‐Lei Shi, Hai‐Zhong Guo, Zhi‐Gang Chen   +8 more
wiley   +1 more source

Enhancement of Dissymmetry Factors Through Axial Elongation of Hetero[n]helicenes

Angewandte Chemie, EarlyView.
A benzofuran scaffolding strategy enables the synthesis of hetero[9]‐, [12]‐, and [15]helicenes. Structural and chiroptical analyses demonstrate that systematic axial elongation increases helical distortion and significantly amplifies luminescence dissymmetry.
Jeppe S. Mogensen, Christoffer Bræstrup, Christoffer Warming, Georg I. Junker, Yann Lie, Mathias Hermann, Kurt V. Mikkelsen, Michael Pittelkow   +7 more
wiley   +2 more sources

UFL1‐Mediated UFMylation of ENO1 Restrains Aerobic Glycolysis and Colorectal Cancer Progression

Advanced Science, EarlyView.
UFMylation restrains aerobic glycolysis and colorectal cancer progression by modifying the glycolytic enzyme ENO1. Pharmacological enhancement of the UFL1–ENO1 interaction by the FDA‐approved antibiotic torezolid promotes ENO1 UFMylation, suppresses tumor metabolism, and sensitizes colorectal cancer to chemotherapy, revealing an actionable metabolic ...
Xiuqing Ma, Rui Wan, Yueyuan Zhong, Ziyang Cui, Xuan Zhang, Xiaoqiang He, Rong Wang, Lei Huang, Wan‐Yang Sun, Rong‐Rong He, Yang Zhou, Jianshuang Li, Li Shen, Shao‐Hua Wang, Tongzheng Liu   +14 more
wiley   +1 more source

A Wagner–Meerwein‐Like Rearrangement Generates a Vinyl Group in the Biosynthesis of the Polychlorinated Lipopeptides Fischerazoles

Angewandte Chemie, EarlyView.
The fischerazoles, unusual cyanobacterial chlorinated lipopeptides are reported. During their biosynthesis, a linear fatty acyl‐acyl carrier protein (ACP) precursor is rearranged through the action of the S‐adenosyl‐methionine (SAM)‐dependent methyltransferase FshF, to generate a pendant vinyl group.
Sandra A. C. Figueiredo, Kathleen Abt, Konrad Viehrig, Peter J. Jervis, Teresa P. Martins, Iñaki Lacomba, Chuhan Li, Daniele Castagnolo, Pedro N. Leão   +8 more
wiley   +2 more sources

Nanometer Layer Coating to Lactose Particles for Optimization of Dexamethasone Pulmonary Delivery

Advanced Science, EarlyView.
Nanometer layer coating (NLC) was investigated and its mechanism illustrated by advanced characterization techniques. The effects and application for the inhalation of dexamethasone (DEX) were demonstrated through in vitro and in vivo evaluation. This approach increased DEX bioavailability and enabled the dose to be reduced to 1/30 of the intravenous ...
Qin Nie, Jiahe Liu, Xiangyu Zhao, Siying Yang, Han Sun, Bingqian Jia, Xiaohong Ren, Xintao Chen, Lixin Sun, Li Wu, Caifen Wang, Jiwen Zhang   +11 more
wiley   +1 more source