Results 61 to 70 of about 470,172 (244)
Impact of Diffraction Data Volume on Data Quality in Serial Crystallography
CrystalsSerial crystallography (SX) enables macromolecular structure determination at biologically relevant temperatures while minimizing radiation damage. This technique relies on processing numerous diffraction images from multiple crystals to construct a ...
Ki Hyun Nam
semanticscholar +1 more source
Journal of the American Chemical Society, 2023 Structural dynamics of water and its hydrogen-bonding networks play an important role in enzyme function via the transport of protons, ions, and substrates. To gain insights into these mechanisms in the water oxidation reaction in Photosystem II (PS II),
M. Doyle +10 more
semanticscholar +1 more source
Nature Communications, 2017 Serial crystallography was developed for protein crystal data collection with X-ray free-electron lasers. Here the authors present several examples which show that serial crystallography using high-viscosity injectors can also be routinely employed for ...
Tobias Weinert +26 more
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Entering an era of dynamic structural biology…
BMC Biology, 2018 A recent paper in BMC Biology presents a general method for mix-and-inject serial crystallography, to facilitate the visualization of enzyme intermediates via time-resolved serial femtosecond crystallography (tr-SFX).
Allen M. Orville
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Effects of Beam Center Position Shifts on Data Processing in Serial Crystallography
CrystalsSerial crystallography (SX) enables the determination of biologically relevant structures at room temperature while minimizing radiation damage. During SX experiments, the beam center on diffraction images can shift due to X-ray beam movements or ...
Ki Hyun Nam
semanticscholar +1 more source
Peptide‐based ligand antagonists block a Vibrio cholerae adhesin
FEBS Letters, EarlyView.The structure of a peptide‐binding domain of the Vibrio cholerae adhesin FrhA was solved by X‐ray crystallography, revealing how the inhibitory peptide AGYTD binds tightly at its Ca2+‐coordinated pocket. Structure‐guided design incorporating D‐amino acids enhanced binding affinity, providing a foundation for developing anti‐adhesion therapeutics ...
Mingyu Wang +9 more
wiley +1 more source
Microcrystal Delivery Using a Syringe and Syringe Pump Method for Serial Crystallography
AnalyticaSerial crystallography (SX) determines the crystal structures of target molecules at room temperature with minimal radiation damage. During SX data collection, the stable delivery of many microcrystals to the X-ray interaction point is crucial for ...
Ki Hyun Nam
semanticscholar +1 more source
Serial Femtosecond Crystallography of Membrane Proteins [PDF]
, 2016 Membrane proteins, including G protein-coupled receptors (GPCRs), constitute the most important drug targets. The increasing number of targets requires new structural information, which has proven tremendously challenging due to the difficulties in growing diffraction-quality crystals.
Lan, Zhu +3 more
openaire +2 more sources
Disordered but rhythmic—the role of intrinsic protein disorder in eukaryotic circadian timing
FEBS Letters, EarlyView.Unstructured domains known as intrinsically disordered regions (IDRs) are present in nearly every part of the eukaryotic core circadian oscillator. IDRs enable many diverse inter‐ and intramolecular interactions that support clock function. IDR conformations are highly tunable by post‐translational modifications and environmental conditions, which ...
Emery T. Usher, Jacqueline F. Pelham
wiley +1 more source
Radiation damage and the case for unpatterned fixed targets
IUCrJAdvances in serial crystallography have reshaped approaches to radiation damage at room temperature. This commentary highlights recent work comparing XFEL and synchrotron experiments, emphasizing its implications for serial crystallography at modern X ...
Dominik Oberthür
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