Results 61 to 70 of about 29,886 (251)

A fixed-target platform for serial femtosecond crystallography in a hydrated environment. [PDF]

, 2020
For serial femtosecond crystallography at X-ray free-electron lasers, which entails collection of single-pulse diffraction patterns from a constantly refreshed supply of microcrystalline sample, delivery of the sample into the X-ray beam path while ...
Barthelmess, M, Batyuk, A, Coleman, MA, Evans, AC, Fischer, P, Frank, M, Gilbile, D, Grant, TD, He, W, Hunter, MS, Kuhl, TL, Meents, A, Pakendorf, T, Segelke, BW, Seuring, C, Shelby, ML   +15 more
core  

A blue light receptor that mediates RNA binding and translational regulation [PDF]

, 2019
Sensory photoreceptor proteins underpin light-dependent adaptations in nature and enable the optogenetic control of organismal behavior and physiology.
Bittl, Robert, Kaiser, Jennifer, Moniot, Sébastien [u.v.m.], Pietruschka, Georg, Pilsl, Sebastian, Renzl, Christian, Schrottke, Stefanie, Sixt, Lisa, Weber, Anna M., Ziegler, Thea   +9 more
core   +1 more source

Shortening injection matrix for serial crystallography [PDF]

Scientific Reports, 2019
Abstract Serial crystallography allows crystal structures to be determined at room temperature through the steady delivery of crystals to the X-ray interaction point. Viscous delivery media are advantageous because they afford efficient sample delivery from an injector or syringe at a low flow rate. Hydrophobic delivery media, such as
openaire   +2 more sources

AAA+ protein unfoldases—the Moirai of the proteome

FEBS Letters, EarlyView.
AAA+ unfoldases are essential molecular motors that power protein degradation and disaggregation. This review integrates recent cryo‐electron microscopy (cryo‐EM) structures and single‐molecule biophysical data to reconcile competing models of substrate translocation.
Stavros Azinas, Marta Carroni
wiley   +1 more source

FELIX: an algorithm for indexing multiple crystallites in X-ray free-electron laser snapshot diffraction images [PDF]

, 2017
A novel algorithm for indexing multiple crystals in snapshot X-ray diffraction images, especially suited for serial crystallography data, is presented.
Adams, Afonine, Agarwal, Barty, Boutet, Chapman, Cornelius Gati, DePonte, Gati, Gildea, Gildea, Ginn, Guo, Hart, Hattne, Henry N. Chapman, Hunter, Ivan G. Kazantsev, Kazantsev, Kazantsev, Kenneth R. Beyerlein, Morawiec, Morawiec, Nicolai Fog-Gade Nielsen, Oleksandr Yefanov, Paithankar, Park, Peter M. Larsen, Philipp, Powell, Powell, Sauter, Schmidt, Schmidt, Sharma, Sierra, Stellato, Søren Schmidt, Sørensen, Thomas A. White, Vaughan, Weierstall, White, White, White, Yefanov, Zaefferer, Zhang, Zhang   +48 more
core   +3 more sources

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

The BAH domain of Rsc2 is a histone H3 binding domain [PDF]

, 2013
Bromo-adjacent homology (BAH) domains are commonly found in chromatin-associated proteins and fall into two classes; Remodels the Structure of Chromatin (RSC)-like or Sir3-like. Although Sir3-like BAH domains bind nucleosomes, the binding partners of RSC-
Adams, Angus-Hill, Anna L. Chambers, Antony W. Oliver, Armache, Badis, Bamford, Bochar, Burrows, Cairns, Cairns, Callebaut, CCP4, Chambers, Chen, Corpet, Davis, DeMase, Desai, Downs, Duncker, Emsley, Ericsson, Faucher, Foster, Gartenberg, Gartenberg, Goodwin, Hickman, Hsu, Huang, Jessica A. Downs, Jones, Kabsch, Kasten, Kent, Kuo, Lacoste, Langst, Laurence H. Pearl, Lee, Leslie, Livingstone, Longtine, McCoy, Mohrmann, Muller, Ng, Noguchi, Norris, Norris, Oliver, Onishi, Park, Peng, Ralhan, Shim, Strahl-Bolsinger, Tanny, Varela, Weissman, Xia   +61 more
core   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

An Optimized Approach for Serial Crystallography Using Chips

Crystals, 2023
Serial crystallography is a rapidly developing method for the determination of the structure of biomolecules at room temperature at near-atomic resolution from an ensemble of small crystals. Numerous advances in detectors, data analysis pipelines, sample
Marina Galchenkova, Aida Rahmani Mashhour, Patrick Y. A. Reinke, Sebastian Günther, Jan Meyer, Henry N. Chapman, Oleksandr M. Yefanov   +6 more
doaj   +1 more source

Hydroxyethyl cellulose matrix applied to serial crystallography [PDF]

, 2019
Serial femtosecond crystallography (SFX) allows structures of proteins to be determined at room temperature with minimal radiation damage. A highly viscous matrix acts as a crystal carrier for serial sample loading at a low flow rate that enables the ...
Hatsui, T., Inoue, S., Iwata, S., Joti, Y., Kameshima, T., Masuda, T., Mizohata, E., Nakane, T., Nakatsu, T., Nango, E., Numata, K., Nureki, O., Song, C., Sugahara, M., Suzuki, M., Tanaka, R., Tono, K., Yabashi, M., Yumoto, F.   +18 more
core   +1 more source