Molecular Imaging using Serial Femtosecond Crystallography
2022Submitted in total fulfilment of the requirements for the degree of Doctor of Philosophy to the Department of Chemistry & Physics, School of Molecular Sciences, La Trobe Institute for Molecular Science, College of Science, Health and Engineering, La Trobe University, Victoria.
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Serial Femtosecond Crystallography (SFX): An Overview
2018X-ray crystallography is a very powerful tool to determine structures of biological macromolecules at the atomic and molecular level and thereby enables insights into their function. Unfortunately, irradiating biological samples with X-rays is an inherently damaging and unavoidable process in conventional X-ray crystallography. The unique properties of
Mark S. Hunter, Petra Fromme
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Microcrystal delivery by pulsed liquid droplet for serial femtosecond crystallography
Acta Crystallographica Section D Structural Biology, 2016A liquid-droplet injector has been developed that delivers pristine microcrystals to an X-ray irradiation area for conducting serial femtosecond crystallography (SFX) with an X-ray free-electron laser (XFEL). By finely tuning the pulsed liquid droplets in time and space, a high hit rate of the XFEL pulses to microcrystals in the droplets was achieved ...
Fumitaka, Mafuné +10 more
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Preparation of RNA Microcrystals for Serial Femtosecond Crystallography Experiments
2022Serial femtosecond crystallography (SFX) experiments using an X-ray free electron laser (XFEL) is a burgeoning method for time-resolved structural studies of biomacromolecules. As with any crystallography experiment, the most important component is quality sample preparation.
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Illumination guidelines for ultrafast pump–probe experiments by serial femtosecond crystallography
Nature Methods, 2020Miriam Stricker +2 more
exaly
Serial time-resolved crystallography of photosystem II using a femtosecond X-ray laser
Nature, 2014Christopher Kupitz +2 more
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Structure of a photosynthetic reaction centre determined by serial femtosecond crystallography
Nature Communications, 2013David Arnlund +2 more
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Grease matrix as a versatile carrier of proteins for serial crystallography
Nature Methods, 2014Michihiro Sugahara +2 more
exaly
Structures of riboswitch RNA reaction states by mix-and-inject XFEL serial crystallography
Nature, 2016John C H Spence, Max O Wiedorn
exaly
Greasing the Path for Serial Femtosecond Crystallography
PSI Structural Genomics Knowledgebase, 2014openaire +1 more source