Variable orthogonality of serine integrase interactions within the ϕC31 family [PDF]
Scientific ReportsSerine integrases are phage- (or mobile element-) encoded enzymes that catalyse site-specific recombination reactions between a short DNA sequence on the phage genome (attP) and a corresponding host genome sequence (attB), thereby integrating the phage ...
Alasdair I. MacDonald +6 more
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Serine integrase chimeras with activity in E. coli and HeLa cells [PDF]
Biology Open, 2014 In recent years, application of serine integrases for genomic engineering has increased in popularity. The factor-independence and unidirectionality of these large serine recombinases makes them well suited for reactions such as site-directed vector ...
Alfonso P. Farruggio, Michele P. Calos
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Attachment site selection and identity in Bxb1 serine integrase-mediated site-specific recombination. [PDF]
PLoS Genetics, 2013 Phage-encoded serine integrases mediate directionally regulated site-specific recombination between short attP and attB DNA sites without host factor requirements.
Shweta Singh +2 more
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There and turn back again: the application of phage serine integrases in eukaryotic systems [PDF]
Frontiers in Bioengineering and BiotechnologySerine integrases (Ints) have gained prominence and have been extensively used in Synthetic Biology due to their ability to modify DNA sequences. Ints are recombinases encoded by the phage genome and have been used to unidirectionally catalyze an ...
Thais Torquato Sales +7 more
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Development of circular AAV cargos for targeted seamless insertion with large serine integrases [PDF]
Molecular Therapy: Methods & Clinical DevelopmentRecent advancements in gene insertion have shifted from DNA-repair-dependent mechanisms to more precise approaches, enhancing safety and predictability for editing outcomes. Integrase-mediated programmable genomic integration (I-PGI) utilizes a DNA cargo
Brett J.G. Estes +12 more
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Increased stable integration efficiency in CHO cells through enhanced nuclear localization of Bxb1 serine integrase [PDF]
BMC BiotechnologyBackground Mammalian display is an appealing technology for therapeutic antibody development. Despite the advantages of mammalian display, such as full-length IgG display with mammalian glycosylation and its inherent ability to select antibodies with ...
Olli Huhtinen +4 more
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Protocol for the establishment of a serine integrase-based platform for functional validation of genetic switch controllers in eukaryotic cells. [PDF]
PLoS ONESerine integrases (Ints) are a family of site-specific recombinases (SSRs) encoded by some bacteriophages to integrate their genetic material into the genome of a host.
Marco A de Oliveira +13 more
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A new-engineered integrative tool to target the terminal compartment of the Streptomyces chromosome [PDF]
Applied Microbiology and BiotechnologyPhages are a valuable resource for the genetic engineering of Streptomyces antibiotic-producing bacteria. Indeed, a few integrative vectors based on phage integrase are available to insert transgenes at specific genomic loci.
Nicolas Delhaye +5 more
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Heterologous Substitution of Mycobacterium tuberculosis rRNA in Mycobacterium smegmatis and Its Impact on Antimicrobial Susceptibility [PDF]
AntibioticsBackground: The global incidence of multidrug-resistant and extensively drug-resistant tuberculosis continues to rise. The ribosome serves as a target for multiple antimicrobials, making functional research on it hold great significance.
Qianwen Yue +4 more
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Single copy optogenetic system for Streptomyces [PDF]
Scientific ReportsLitR is a blue-green light-sensing transcriptional regulator that uses coenzyme B12 as a chromophore. In this study, we developed a genome-integrative light-inducible expression (iLiEX) system in Streptomyces griseus NBRC 13350, a Gram-positive bacterium
Airi Watanabe +3 more
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