Results 21 to 30 of about 5,999 (124)

Cross-talk between Diverse Serine Integrases [PDF]

Journal of Molecular Biology, 2014
Phage-encoded serine integrases are large serine recombinases that mediate integrative and excisive site-specific recombination of temperate phage genomes. They are well suited for use in heterologous systems and for synthetic genetic circuits as the attP and attB attachment sites are small (
Shweta, Singh, Kate, Rockenbach, Rebekah M, Dedrick, Andrew P, VanDemark, Graham F, Hatfull   +4 more
openaire   +2 more sources

Tight regulation of the intS gene of the KplE1 prophage: a new paradigm for integrase gene regulation. [PDF]

PLoS Genetics, 2010
Temperate phages have the ability to maintain their genome in their host, a process called lysogeny. For most, passive replication of the phage genome relies on integration into the host's chromosome and becoming a prophage.
Gaël Panis, Yohann Duverger, Elise Courvoisier-Dezord, Stéphanie Champ, Emmanuel Talla, Mireille Ansaldi   +5 more
doaj   +1 more source

Large serine integrases utilise scavenged phage proteins as directionality cofactors. [PDF]

Nucleic Acids Res
Abstract Recombination directionality factors (RDFs) for large serine integrases (LSIs) are cofactor proteins that control the directionality of recombination to favour excision over insertion. Although RDFs are predicted to bind their cognate LSIs in similar ways, there is no overall common structural theme across LSI RDFs, leading ...
Alsaleh A, Holland A, Shin H, Reyes TP, Baksh A, Taiwo-Aiyerin OT, Pigli Y, Rice PA, Olorunniji FJ.   +8 more
europepmc   +5 more sources

The ΦBT1 large serine recombinase catalyzes DNA integration at pseudo-attB sites in the genus Nocardia [PDF]

PeerJ, 2018
Plasmid vectors based on bacteriophage integrases are important tools in molecular microbiology for the introduction of foreign DNA, especially into bacterial species where other systems for genetic manipulation are limited.
Marion Herisse, Jessica L. Porter, Romain Guerillot, Takehiro Tomita, Anders Goncalves Da Silva, Torsten Seemann, Benjamin P. Howden, Timothy P. Stinear, Sacha J. Pidot   +8 more
doaj   +2 more sources

Mathematical model of a serine integrase-controlled toggle switch with a single input. [PDF]

J R Soc Interface, 2018
Dual-state genetic switches that can change their state in response to input signals can be used in synthetic biology to encode memory and control gene expression. A transcriptional toggle switch (TTS), with two mutually repressing transcription regulators, was previously used for switching between two expression states.
Pokhilko A, Ebenhöh O, Stark WM, Colloms SD.   +3 more
europepmc   +5 more sources

Mutational analysis of highly conserved residues in the phage phiC31 integrase reveals key amino acids necessary for the DNA recombination. [PDF]

PLoS ONE, 2010
BACKGROUND: Amino acid sequence alignment of phage phiC31 integrase with the serine recombinases family revealed highly conserved regions outside the catalytic domain. Until now, no system mutational or biochemical studies have been carried out to assess
Shaohui Liu, Jinfang Ma, Wei Wang, Maoxiang Zhang, Qingting Xin, Siman Peng, Rongxiu Li, Huanzhang Zhu   +7 more
doaj   +1 more source

Tetrameric Structure of a Serine Integrase Catalytic Domain [PDF]

Structure, 2008
The serine integrases have recently emerged as powerful new chromosome engineering tools in various organisms and show promise for therapeutic use in human cells. The serine integrases are structurally and mechanistically unrelated to the bacteriophage lambda integrase but share a similar catalytic domain with the resolvase/invertase enzymes typified ...
Yuan, Peng, Gupta, Kushol, Van Duyne, Gregory D.   +2 more
openaire   +2 more sources

Isolation and characterization of Streptomyces bacteriophages and Streptomyces strains encoding biosynthetic arsenals.

PLoS ONE, 2022
The threat to public health posed by drug-resistant bacteria is rapidly increasing, as some of healthcare's most potent antibiotics are becoming obsolete.
Elizabeth T Montaño, Jason F Nideffer, Lauren Brumage, Marcella Erb, Julia Busch, Lynley Fernandez, Alan I Derman, John Paul Davis, Elena Estrada, Sharon Fu, Danielle Le, Aishwarya Vuppala, Cassidy Tran, Elaine Luterstein, Shivani Lakkaraju, Sriya Panchagnula, Caroline Ren, Jennifer Doan, Sharon Tran, Jamielyn Soriano, Yuya Fujita, Pranathi Gutala, Quinn Fujii, Minda Lee, Anthony Bui, Carleen Villarreal, Samuel R Shing, Sean Kim, Danielle Freeman, Vipula Racha, Alicia Ho, Prianka Kumar, Kian Falah, Thomas Dawson, Eray Enustun, Amy Prichard, Ana Gomez, Kanika Khanna, Shelly Trigg, Kit Pogliano, Joe Pogliano   +40 more
doaj   +2 more sources

Control of phage Bxb1 excision by a novel recombination directionality factor. [PDF]

PLoS Biology, 2006
Mycobacteriophage Bxb1 integrates its DNA at the attB site of the Mycobacterium smegmatis genome using the viral attP site and a phage-encoded integrase generating the recombinant junctions attL and attR.
Pallavi Ghosh, Laura R Wasil, Graham F Hatfull   +2 more
doaj   +1 more source

High fidelity one‐pot DNA assembly using orthogonal serine integrases

Biotechnology Journal, 2022
AbstractBackgroundLarge serine integrases (LSIs, derived from temperate phages) have been adapted for use in a multipart DNA assembly process in vitro, called serine integrase recombinational assembly (SIRA). The versatility, efficiency, and fidelity of SIRA is limited by lack of a sufficient number of LSIs whose activities have been characterized in ...
Jumai Abioye, Makeba Lawson‐Williams, Alicia Lecanda, Brecken Calhoon, Arlene L. McQue, Sean D. Colloms, W. Marshall Stark, Femi J. Olorunniji   +7 more
openaire   +2 more sources