Results 31 to 40 of about 5,999 (124)
Control of directionality in Streptomyces phage φBT1 integrase-mediated site-specific recombination. [PDF]
PLoS ONE, 2013 Streptomyces phage φBT1 integrates its genome into the attB site of the host chromosome with the attP site to generate attL and attR. The φBT1 integrase belongs to the large serine recombinase subfamily which directly binds to target sites to initiate ...
Lin Zhang +4 more
doaj +1 more source
Genetic switches designed for eukaryotic cells and controlled by serine integrases [PDF]
Communications Biology, 2020 AbstractRecently, new serine integrases have been identified, increasing the possibility of scaling up genomic modulation tools. Here, we describe the use of unidirectional genetic switches to evaluate the functionality of six serine integrases in different eukaryotic systems: the HEK 293T cell lineage, bovine fibroblasts and plant protoplasts ...
Mayna S. Gomide +17 more
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Integration and excision by the large serine recombinase φRv1 integrase [PDF]
Molecular Microbiology, 2005 SummaryThe Mycobacterium tuberculosis prophage‐like element φRv1 encodes a site‐specific recombination system utilizing an integrase of the serine recombinase family. Recombination occurs between a putative attP site and the host chromosome, but is unusual in that the attB site lies within a redundant repetitive element (REP13E12) of which there ...
Lori A, Bibb +2 more
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Making serine integrases work for us [PDF]
Current Opinion in Microbiology, 2017 DNA site-specific recombinases are enzymes (often associated with mobile DNA elements) that catalyse breaking and rejoining of DNA strands at specific points, thereby bringing about precise genetic rearrangements. Serine integrases are a group of recombinases derived from bacteriophages.
openaire +2 more sources
Mathematical modelling of serine integrase - mediated gene assembly [PDF]
, 2018 AbstractSite-specific recombination promoted by serine integrases can be used for ordered assembly of DNA fragments into larger arrays. When a plasmid vector is included in the assembly, the circular product DNA molecules can transformE. colicells. A convenient “one-pot” method using a single integrase involves recombination between pairs of matched ...
Pokhilko, Alexandra +3 more
openaire +1 more source
Scientific Reports, 2022 Streptomycetes are ubiquitous soil bacteria. Here we report the complete and annotated genome sequence and characterization of Streptomyces phage Pablito, isolated from a soil sample in Haarlem, the Netherlands using Streptomyces lividans as host.
Véronique Ongenae +5 more
doaj +1 more source
GCN2 phosphorylates HIV-1 integrase and decreases HIV-1 replication by limiting viral integration
Scientific Reports, 2017 GCN2 is a serine/threonine kinase involved in cellular stress response related to amino acid starvation. Previously, we showed that GCN2 interacts with HIV-1 integrase and is activated during HIV-1 infection.
A. Jaspart +13 more
doaj +1 more source
Rapid metabolic pathway assembly and modification using serine integrase site-specific recombination. [PDF]
Nucleic Acids Res, 2014 Abstract Synthetic biology requires effective methods to assemble DNA parts into devices and to modify these devices once made. Here we demonstrate a convenient rapid procedure for DNA fragment assembly using site-specific recombination by ϕC31 integrase.
Colloms SD +7 more
europepmc +7 more sources
Mechanistic modelling of a recombinase-based two-input temporal logic gate
Engineering Biology, 2017 Site-specific recombinases (SSRs) mediate efficient manipulation of DNA sequences in vitro and in vivo. In particular, serine integrases have been identified as highly effective tools for facilitating DNA inversion, enabling the design of genetic ...
Jack E. Bowyer +3 more
doaj +1 more source
Attachment site recognition and regulation of directionality by the serine integrases [PDF]
Nucleic Acids Research, 2013 Abstract Serine integrases catalyze the integration of bacteriophage DNA into a host genome by site-specific recombination between ‘attachment sites’ in the phage ( attP ) and the host ( attB ). The reaction is highly directional; the reverse excision reaction between the product attL and attR sites does not occur in the absence of a ...
Rutherford, Karen +4 more
openaire +2 more sources