Optimization of Serine Protease Purification from Mango (Mangifera indica cv. Chokanan) Peel in Polyethylene Glycol/Dextran Aqueous Two Phase System [PDF]
, 2012 Mango peel is a good source of protease but remains an industrial waste. This study focuses on the optimization of polyethylene glycol (PEG)/dextran-based aqueous two-phase system (ATPS) to purify serine protease from mango peel.
Abdul Manap Mohd Yazid +32 more
core +4 more sources
Serine protease from Indian Cobra venom: its anticoagulant property and effect on human fibrinogen [PDF]
, 2020 An unusual low molecular weight serine protease (NnP28) has been purified from Indian Cobra (Naja naja) venom from the western region of the Indian sub-continent.
Divya Kumar +6 more
core +1 more source
The HtrA family of serine proteases [PDF]
Molecular Microbiology, 1997 HtrA, also known as DegP and probably identical to the Do protease, is a heat shock‐induced serine protease that is active in the periplasm of Escherichia coli. Homologues of HtrA have been described in a wide range of bacteria and in eukaryotes. Its chief role is to degrade misfolded proteins in the periplasm.
Mark J. Pallen, Brendan W. Wren
openaire +3 more sources
Heliyon, 2021 In this study, we have isolated and characterized proteolytic soil bacteria and their alkaline protease. Based on 16S rRNA sequence analysis, 12 isolates with the highest protease activity were classified as B. subtilis and B. cereus groups. B.
Amal Mahmoud +5 more
doaj
Biology, 2020 An increased need by the green industry for enzymes that can be exploited for eco-friendly industrial applications led us to isolate and identify a unique protease obtained from a proteolytic Bacillus megaterium-TK1 strain from a seawater source.
Tamilvendan Manavalan +3 more
doaj +1 more source
Isolation, purification, characterization and applications of serine protease from Bacillus megaterium [PDF]
, 2010 Bacillus megaterium isolated from poultry farm soil was identified by standard biochemical tests and screened for the production of serine protease. Production of serine protease was done using 5 different medias by varying the type of amino acid added ...
Deepa, Deepa +4 more
core +2 more sources
The impact of physiological stress conditions on protein structure and trypsin inhibition of serine protease inhibitor Kazal type 1 (SPINK1) and its N34S variant [PDF]
, 2020 One of the most common mutations in the serine protease inhibitor Kazal type 1 (SPINK1) gene is the N34S variant which is strongly associated with chronic pancreatitis. Although it is assumed that N34S mutation constitutes a high-risk factor, the underlying pathologic mechanism is still unknown.
arxiv +1 more source
The mitochondrial protease HtrA2 restricts the NLRP3 and AIM2 inflammasomes. [PDF]
, 2018 Activation of the inflammasome pathway is crucial for effective intracellular host defense. The mitochondrial network plays an important role in inflammasome regulation but the mechanisms linking mitochondrial homeostasis to attenuation of inflammasome ...
Alnemri, Emad S. +9 more
core +3 more sources
BMC Evolutionary Biology, 2018 Background The ascomycete fungus Clonostachys rosea (order Hypocreales) can control several important plant diseases caused by plant pathogenic fungi and nematodes. Subtilisin-like serine proteases are considered to play an important role in pathogenesis
Mudassir Iqbal +5 more
doaj +1 more source
Amino acid frequency and domain features serve well for random forest based classification of thermophilic and mesophilic protein; a case study on serine proteases [PDF]
arXiv, 2021 Thermostability is an important prerequisite for enzymes employed for industrial applications. Several machine learning based models have thus been formulated for protein classification based on this particular trait. These models have employed features derived from sequences, structures or both resulting in a >93% accuracy based on a 10-fold cross ...
arxiv