Results 91 to 100 of about 134,111 (268)

Challenges of Using Molecular Serotyping for Surveillance of Pneumococcal Disease

Journal of Clinical Microbiology, 2014
Recent advances in the molecular identification and serotyping of Streptococcus pneumoniae are useful for culture-negative samples; however, there are limitations associated with these methods.
Victoria Magomani, N. Wolter, S. Tempia, M. du Plessis, L. De Gouveia, A. von Gottberg   +5 more
semanticscholar   +1 more source

Disruption of the blood–brain barrier contributes to neurobehavioral changes observed in rheumatic heart disease

Animal Models and Experimental Medicine, EarlyView.
Sydenham's chorea (SC) is the neurological manifestation associated with acute rheumatic fever (ARF), triggered by a group A streptococcal (GAS) infection. Anti‐GAS antibodies cross‐reacting with tissue proteins are implicated in the development of neurological changes. In this study, using an animal model, we demonstrate that a compromised blood–brain
Rukshan Ahamed Mohamed Rafeek, Riya Thapa, Samarjeet Saluja, Bipandeep Banga, David McMillan, Kadaba Sri Sriprakash, Nicholas M. Andronicos, Adam Hamlin, Natkunam Ketheesan   +8 more
wiley   +1 more source

Capsule Typing of Haemophilus influenzae by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

Emerging Infectious Diseases, 2018
Encapsulated Haemophilus influenzae strains belong to type-specific genetic lineages. Reliable capsule typing requires PCR, but a more efficient method would be useful. We evaluated capsule typing by using matrix-assisted laser desorption/ionization time-
Viktor Månsson, Janet R. Gilsdorf, Gunnar Kahlmeter, Mogens Kilian, J. Simon Kroll, Kristian Riesbeck, Fredrik Resman   +6 more
doaj   +1 more source

Diversity in the Major Polysaccharide Antigen of Acinetobacter Baumannii Assessed by DNA Sequencing, and Development of a Molecular Serotyping Scheme

PLoS ONE, 2013
We have sequenced the gene clusters for type strains of the Acinetobacter baumannii serotyping scheme developed in the 1990s, and used the sequences to better understand diversity in surface polysaccharides of the genus.
Dalong Hu, B. Liu, L. Dijkshoorn, Lei Wang, P. Reeves   +4 more
semanticscholar   +1 more source

Perfusion‐Based Production of rAAV via an Intensified Transient Transfection Process

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Increasing demand for recombinant adeno‐associated virus (rAAV)‐based gene therapies necessitates increased manufacturing production. Transient transfection of mammalian cells remains the most commonly used method to produce clinical‐grade rAAVs due to its ease of implementation.
Tam N. T. Nguyen, Damdae Park, Christopher T. Canova, Jose Sangerman, Prasanna Srinivasan, Rui Wen Ou, Paul W. Barone, Caleb Neufeld, Jacqueline M. Wolfrum, Stacy L. Springs, Anthony J. Sinskey, Richard D. Braatz   +11 more
wiley   +1 more source

Two variants of HIV-1 B serotype are transmitted heterosexually in São Paulo, Brazil

Brazilian Journal of Medical and Biological Research, 1998
HIV-1 variability may have an important impact on transmission and pathogenicity. Better characterization of the HIV epidemic in Brazil is necessary for the development of vaccine trials in this country.
Casseb J., Hong M.A., Gonsalez C., Brígido L.F., Duarte A.J.S., Michael-Hendry R.   +5 more
doaj  

Desarrollo y validación de una reacción en cadena de la polimerasa múltiple para la identificación de los serogrupos B, C2, D y E de Salmonella enterica

Biomédica: revista del Instituto Nacional de Salud, 2009
Introducción. El esquema Kaufmann-White para la serotipificación de Salmonella, reconoce 46 antígenos O y 119 antígenos H, los cuales han permitido la caracterización de 2.541 serovares.
Nora Cardona-Castro, Lelia Lavalett, Miryan Margot Sánchez, Nélida Múñoz, Jaime Moreno   +4 more
doaj   +1 more source

Single-Reaction, Multiplex, Real-Time RT-PCR for the Detection, Quantitation, and Serotyping of Dengue Viruses

PLoS Neglected Tropical Diseases, 2013
Background Dengue fever results from infection with one or more of four different serotypes of dengue virus (DENV). Despite the widespread nature of this infection, available molecular diagnostics have significant limitations.
J. Waggoner, J. Abeynayake, M. Sahoo, L. Gresh, Y. Téllez, Karla González, Gabriela Ballesteros, A. Pierro, P. Gaibani, F. Guo, V. Sambri, Á. Balmaseda, Kumudu Karunaratne, E. Harris, B. Pinsky   +14 more
semanticscholar   +1 more source

Development of an HEK293 Suspension Cell Culture Medium, Transient Transfection Optimization Workflow, and Analytics for Batch rAAV Manufacturing

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Recombinant adeno associated virus (rAAV) vectors have become popular delivery vehicles for in vivo gene therapies, but demand for rAAVs continues to outpace supply. Platform processes for rAAV production are being developed by many manufacturers, and transient chemical transfection of human embryonic kidney 293 (HEK293) cells is currently the
Erica A. Green, Qiang Fu, Nelson Ndhairo, Thomas M. Leibiger, Yongdan Wang, Yongsuk Lee, Kelvin H. Lee, Michael Betenbaugh, Seongkyu Yoon, David J. McNally   +9 more
wiley   +1 more source

Serotyping of Toxoplasma gondii in Cats (Felis domesticus) Reveals Predominance of Type II Infections in Germany

PLoS ONE, 2013
Background Cats are definitive hosts of Toxoplasma gondii and play an essential role in the epidemiology of this parasite. The study aims at clarifying whether cats are able to develop specific antibodies against different clonal types of T.
P. Maksimov, J. Zerweck, J. Dubey, N. Pantchev, C. Frey, A. Maksimov, U. Reimer, M. Schutkowski, M. Hosseininejad, M. Ziller, F. Conraths, G. Schares   +11 more
semanticscholar   +1 more source