Results 101 to 110 of about 134,111 (268)
Biotechnology Progress, EarlyView.Abstract The possibility to produce recombinant adeno‐associated virus (rAAV) by adherent HEK293T cells was studied in a stirred tank bioreactor (STR) culture of cell aggregates. A proof‐of‐concept of rAAV production was successfully demonstrated in a process where single cells were first expanded, then cell aggregates were formed by dilution into a ...
Brian Ladd +2 more
wiley +1 more source
SEROTYPING FOR HOMOTRANSPLANTATION XVIII. REFINEMENT OF MICRODROPLET LYMPHOCYTE CYTOTOXICITY TEST
Transplantation, 1968 The microdroplet lymphocyte cytotoxicity test was examined thoroughly in an effort to increase the reproducibility of the test. The discrepancy rate in a large series of tests was reduced from 5.16% at the start of this study to the present 0.95% by ...
K. Mittal +3 more
semanticscholar +1 more source
Iterative hybrid model based optimization of rAAV production
Biotechnology Progress, EarlyView.Abstract Changes in serotype or genetic payload of recombinant adeno associated virus (rAAVs) gene therapies require adapting the transfection conditions of the upstream HEK293 cultivations. This study adopts an iterative model‐based experiment design approach, where increasing data availability is leveraged to evolve models of different complexity ...
Claudio Müller +7 more
wiley +1 more source
Cell Proliferation, Volume 55, Issue 12, December 2022., 2022 Transduction efficiency and immune responses hinder the application of recombinant adeno‐associated viral (rAAV) vectors in gene therapy. Here, Han et al. describe a new combined strategy for AAV capsid engineering and obtain several AAV variants with higher liver transduction efficiency and less immune activation.
Jiabao Han +9 more
wiley +1 more source
South African Journal of Science, 2010 The occurrence and distribution of Shigella species in water from the five river catchments in the North West province of South Africa were investigated.
Constance Wose Kinge, Moses Mbewe
doaj +2 more sources
Production of latex agglutination reagents for pneumococcal serotyping
BMC Research Notes, 2013 BackgroundThe current ‘gold standard’ for serotyping pneumococci is the Quellung test. This technique is laborious and requires a certain level of training to correctly perform.
B. Ortika +4 more
semanticscholar +1 more source
A synthetic platform for developing recombinant adeno‐associated virus type 8 producer cell lines
Biotechnology Progress, EarlyView.Abstract Recombinant adeno‐associated virus (rAAV) is one of the most widely used viral vectors for gene therapy. It is used in very high doses for the treatment of many diseases, making large‐scale production for clinical applications challenging. We have established a synthetic biology‐based platform to construct stable production cell lines, which ...
Yu‐Chieh Lin +5 more
wiley +1 more source
Serotyping of Streptococcus pneumoniae Based on Capsular Genes Polymorphisms
PLoS ONE, 2013 Streptococcus pneumoniae serotype epidemiology is essential since serotype replacement is a concern when introducing new polysaccharide-conjugate vaccines.
F. Raymond +7 more
semanticscholar +1 more source
Chemistry – A European Journal, EarlyView.Staphylococcus aureus serotype 1 is a highly virulent pathogen and the capsular polysaccharide (CP1) has been shown to play an important role in virulence and be a target for opsonic antibodies. We here describe the synthesis of a set of CP1 oligosaccharides, varying in length and with different taurination patterns. Abstract Staphylococcus aureus is a
Kitt Emilie Østerlid +4 more
wiley +1 more source
New pyridobenothiazolone derivatives display nanomolar pan‐serotype anti‐DENV activity
ChemMedChem, Accepted Article.Dengue virus (DENV) serotypes 1–4 are mosquito‐borne flaviviruses that are responsible for significant morbidity and mortality worldwide, particularly in tropical and subtropical regions. Although two vaccines have been approved, their unbalanced efficacy across serotypes poses potential risks for specific populations.
Tommaso Felicetti +13 more
wiley +1 more source