Using fluorescence fluctuation spectroscopy to quantify molecular dynamics within cells
Microscopy techniques like fluorescence correlation spectroscopy (FCS) and number and brightness analysis (N&B) allow to quantify concentrations, diffusion rates, and oligomerization of fluorescently labeled molecules in the living cell. In this protocol the principles of how to set up these types of experiments are described including calibration ...
Mark A. Hink
wiley +1 more source
In vitro generation of regulatory T cells: A challenging tool for studying immune aging in humans
In vitro induction of human regulatory T cells using standard protocols generates suppressive, Foxp3‐expressing CD4+ T cells independently of TGF‐β supplementation and donor age. Regulatory T (Treg) cells play a key role in immune tolerance and homeostasis.
Lynn Muller +3 more
wiley +1 more source
Serum biochemistry, hematology, and reproductive behavior of free-roaming cats in urban and rural habitats. [PDF]
Islam F +15 more
europepmc +1 more source
This minireview explores fluorination as a key strategy to optimize therapeutic molecules and nanocarriers. By incorporating different fluorinated motifs—from single atoms to linear and branched chains—drug delivery, cellular uptake, and colloidal stability can be improved and fine‐tuned.
Martina Beccalli +2 more
wiley +1 more source
Effects of bile acids on production performance, serum biochemistry, lipid metabolism, and intestinal morphology in broilers. [PDF]
Liu W +8 more
europepmc +1 more source
Sertraline Treatment Can Mimic Niemann‐Pick Type C Biomarker Profile: A Diagnostic Pitfall
ABSTRACT Background Oxysterols (cholestane‐3β,5α,6β‐triol and 7‐ketocholesterol) and N‐palmitoyl‐O‐phosphocholineserine (PPCS) are sensitive biomarkers for Niemann‐Pick disease type C (NPC) screening. However, false‐positive results occur, with a biomarker profile suggestive of NPC despite the absence of pathogenic variants in genes involved in NPC or ...
Maria Makrygianni +19 more
wiley +1 more source
Unlocking the Potential of Paper Mulberry Powder in Cherry Valley Ducks: Impacts on Growth, Serum Biochemistry, and Cecum Microbiome. [PDF]
Xiong Y, Tang C, Wang X, Wang Y, Yang F.
europepmc +1 more source
Interactive impact of salinity and oxygen level on the growth performance, digestive enzymes, serum biochemistry, antioxidative, immunity, and histological status of Nile tilapia (Oreochromis niloticus). [PDF]
Monier MN +7 more
europepmc +1 more source
Effects of fermented ramie feed on the growth performance, serum biochemistry, metabolic capacity, antioxidant capacity, and intestinal health of <i>Linwu</i> ducks. [PDF]
Lv Z +9 more
europepmc +1 more source

