Results 91 to 100 of about 94,352 (268)

Spatiotemporal Climate–Vegetation Dynamics and the Vegetation Ecological Quality-Based Zoning Under Climate Change: Evidence from the Qinling Mountains

open access: yesLand
This study analyzed the spatiotemporal dynamics of the vegetation ecological quality under climate change. Focusing on the vegetation conditions, a vegetation ecological quality index was constructed, expressing as the product of vegetation fraction ...
Yishan Xu   +7 more
doaj   +1 more source

Sudden anaerobization in Amphibacillus xylanus increases intracellular labile ferrous iron and inhibits cell growth

open access: yesFEBS Open Bio, EarlyView.
Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata   +13 more
wiley   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

open access: yesFEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu   +5 more
wiley   +1 more source

Time‐restricted feeding prior to Mycobacterium tuberculosis infection reduces tissue CD4+ T cells with limited impact on bacterial clearance

open access: yesFEBS Open Bio, EarlyView.
Time‐restricted feeding (TRF) in mice increased liver fatty acid oxidation and decreased fatty acid biosynthesis. These alterations persisted when TRF was discontinued and the host was infected with Mycobacterium tuberculosis. Pre‐exposure to TRF did not alter tissue (lung and spleen) mycobacterial burden but significantly reduced CD3+ T cells in lungs
Ashish Gupta   +7 more
wiley   +1 more source

The crystal structure of the Borrelia burgdorferi nicotinamidase BBE22 resolves a long‐standing annotation error

open access: yesFEBS Open Bio, EarlyView.
The crystal structure of Borrelia burgdorferi nicotinamidase (PncA/BBE22) reveals the correct full‐length protein initiated from a non‐canonical AUU start codon. The structure validates previous biochemical findings and resolves a long‐standing annotation error, demonstrating that the truncated database sequence is structurally incompatible with the ...
Kalvis Brangulis
wiley   +1 more source

From Traditional Settlements to Arrival Cities: A Study on Contemporary Residential Patterns in Chinese Siheyuan

open access: yesBuildings
With the rapid expansion of Chinese cities, traditional siheyuan in some old urban areas and historic districts have gradually transformed into overcrowded courtyard tenements inhabited primarily by rural migrants.
Mengying Wang   +3 more
doaj   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

open access: yesFEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova   +2 more
wiley   +1 more source

Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes

open access: yesFEBS Open Bio, EarlyView.
Group A Streptococcus (GAS) acquires covRS mutations driving a hypervirulent bacterial state, frequently associated with invasive disease‐like necrotizing fasciitis. We demonstrate that the newly emerged M1UK GAS lineage can also acquire these mutations.
Jarrad Pritchard   +12 more
wiley   +1 more source

Artificial intelligence in fabric design: a critical review of technological advancements and socio-creative implications (2019–2024)

open access: yesHumanities & Social Sciences Communications
Artificial Intelligence is revolutionizing the textile and fashion sectors. Machine learning and deep learning are fundamentally changing the fabric design process, which has always relied on intuitive yet laborious hand-crafted steps, such as surface ...
Asmaa. A. H. El-Bassuony   +2 more
doaj   +1 more source

The Influences of Shade and Non-Uniform Heating of Building Walls on Micro-Environments Within Urban Street Canyons and Their Planning Implications

open access: yesBuildings
Urbanization and climate change intensify urban heat islands and air pollution; therefore, street canyon building planning that accounts for road orientation, shading, thermal environment, and ventilation is crucial. This study uses numerical simulations
Wen Xu   +5 more
doaj   +1 more source

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