Results 231 to 240 of about 17,254 (263)

Noninvasive measurement of dissolved oxygen in shake flasks

Biotechnology and Bioengineering, 2002
AbstractShake flasks are ubiquitous in cell culture and fermentation. However, conventional devices for measuring oxygen concentrations are impractical in these systems. Thus, there is no definitive information on the oxygen supply of growing cells. Here we report the noninvasive, nonintrusive monitoring of dissolved oxygen (DO) in shake flasks using a
Yordan Kostov, Leah Tolosa, Govind Rao, Peter Harms   +3 more
openaire   +2 more sources

Model for oxygen transfer in a shake flask

Biotechnology and Bioengineering, 1978
AbstractThe shake flask is a very common and useful tool for the study of submerged fermentations on a small scale. However, the oxygen supply may easily become a limiting factor. A model for the aeration in shake flasks is presented that enables one to predict whether in the course of an experiment the oxygen supply is becoming a growth‐limiting ...
N. W. F. Kossen, J. C. van Suijdam, A. C. Joha   +2 more
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Volumetric power consumption in baffled shake flasks

Chemical Engineering Science, 2006
Abstract For the cultivation of microorganisms, baffled shake flasks are employed when increased levels of oxygenation and mixing are required. Their use has been discouraged, however, due to the danger of a wetted sterile plug and the lower reproducibility of the experimental results.
Yusuke Suzuki, Cyril P. Peter, Jochen Büchs, Stefan Lotter, Kirill Rachinskiy   +4 more
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Measurement of the evaporation rate of liquid in a shaking flask

Journal of Fermentation Technology, 1987
Abstract The evaporation rate ( N H2O ) of liquid in a shaking flask was measured under various shaking conditions: temperature, humidity, flask shape, liquid volume in the flask ( V L ), length of the stopper in the flask neck ( L C ), rotational speed of the shaker ( N ), and wind velocity ( V W ).
Shun-ichi Akiyama, Yasuhiro Sumino
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Validating shaking flasks as representative screening systems

Biochemical Engineering Journal, 2004
The high information demand in biotechnical process development makes shaken bioreactors an essential tool for process development. By far most of all biotechnological experiments are carried out in shaken bioreactors. Nevertheless, the reaction conditions in these systems often are not representative of the following conditions of the technical scale.
Andreas Künkel, Stephan A. Freyer, Matthia König   +2 more
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Parallel substrate feeding and pH-control in shaking-flasks

Biochemical Engineering Journal, 2001
An intermittent feeding system for shaking-flasks was developed to close the gap between batch operated shaking-flasks and fed-batch operated as well as pH-controlled stirred tank reactors. A precise syringe pump was connected via a substrate distribution system to individual 2/2-way miniature valves, one for each of up to 16 shaking-flask. The shaking-
Weuster-Botz, Dirk, Altenbach-Rehm, Jutta, Arnold, Matthias   +2 more
openaire   +4 more sources

Reinventing shake flask fermentation: The breathable flask

Biotechnology and Bioengineering
AbstractCultivating cells in shake flasks is a routine operation that is largely unchanged since its inception. A glass or plastic Erlenmeyer vessel with the primary gas exchange taking place across various porous plugs is used with media volumes typically ranging from 100 mL to 2 L.
Vikash Kumar, Michael Tolosa, Xudong Ge, Govind Rao   +3 more
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Sphalerite bioleaching comparison in shake flasks and percolators

Minerals Engineering, 2019
Abstract Bioleaching experiments with a mixed Acidithiobacillus-culture were carried out in shake flasks as well as in column percolators with crushed and sieved sphalerite-rich ore from a former mine in the German Harz Mountains. Efficient sphalerite dissolution in the bioleaching assays was observed in contrast to the chemical leaching experiments.
Jens Stummeyer, Axel Schippers, Torsten Graupner, Christoph Tanne   +3 more
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Measurement and characterization of mixing time in shake flasks

Chemical Engineering Science, 2011
Abstract As yet, investigations on mixing time have been focused on small and large scale stirred tanks. The aim of this study was to develop a new method for characterizing the mixing process and quantitatively measuring the mixing time in shake flasks by introducing a rotating camera for online observation of the fluid flow in combination with a ...
Werner Eberhard, Rung-Kai Tan, Jochen Büchs   +2 more
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In situ pH maintenance for mammalian cell cultures in shake flasks and tissue culture flasks

Biotechnology Progress, 2012
AbstractpH in animal cell cultures decreases due to production of metabolites like lactate. pH control via measurement and base addition is not easily possible in small‐scale culture formats like tissue‐culture flasks and shake flasks. A hydrogel‐based system is reported for in situ pH maintenance without pH measurement in such formats, and is ...
Ketaki Pradhan, Mugdha Gadgil, Tejal Pant   +2 more
openaire   +3 more sources