Results 241 to 250 of about 17,254 (263)

Power consumption in shaking flasks on rotary shaking machines: I. Power consumption measurement in unbaffled flasks at low liquid viscosity

Biotechnology and Bioengineering, 2000
In this first article of a series a new method is introduced that enables the accurate determination of the power consumption in a shaking flask. The method is based on torque measurements in the drive and appropriate compensation of the friction losses.
Claudia Milbradt, Bernd Zoels, Jochen Büchs, Ulrike Maier   +3 more
openaire   +3 more sources

High-aeration capacity shake-flask system

Journal of Fermentation and Bioengineering, 1989
Abstract A novel shake-flask designed for high-aeration capacity, the Tunair flask system, was found to be superior to the currently available shake-flasks in the cultivation of selected microorganisms. The significant features of the flasks include wide-mouth opening, barrel-shaped trunk, two battle types, and dome-shaped closure with vanes.
openaire   +2 more sources

Evaluation and development of static shake-flask biodegradation test systems [PDF]

Environmental Toxicology and Chemistry, 1983
Abstract Several biodegradation flask systems were evaluated to assess the effects of flask shape and volume and length of incubation on the recovery of 14CO2. During a 6-h incubation period, it was demonstrated that the recovery of 14 CO2 decreased as the volume of the flask (50 ml to 2 L) and the length of the diffusion path increased.
James E. Blum, Dennis M. Stainken, Cathy A. Stevenson   +2 more
openaire   +1 more source

Suitability of the shaking flask for oxygen supply to microbiological cultures

Bioprocess Engineering, 1991
Due of its simplicity the shaking flask is used in serial studies, e.g. in the screening for secondary metabolites or in the optimization of fermentation processes. Experimental investigations in these small bioreactors are often the first step in developing a large-scale fermentation process.
M. Schedel, H. J. Henzler
openaire   +2 more sources

High-yield shake-flask fermentation of xylose to ethanol

Applied Biochemistry and Biotechnology, 1994
Methods of achieving high-yield fermentation ofd-xylose to ethanol in shake flasks were investigated using the microaerophilic yeastPichia stipitis NRRL Y-7124. Ethanol yields based on consumed xylose approached the theoretical maximum of 0.51 g/g at high culture volumes.
Brian L. Boynton, James D. McMillan
openaire   +2 more sources

CFD analysis of the turbulent flow in baffled shake flasks

Biochemical Engineering Journal, 2013
Abstract In this work, computational fluid dynamics (CFD) technique is used to simulate the complicated unsteady-state turbulent flow field formed in baffled flask. The baffled flask shows advantages both in mass transfer capacity and in shear formation in comparison with unbaffled flasks.
Siliang Zhang, Jianye Xia, Yonghong Wang, Ju Chu, Chao Li, Yingping Zhuang   +5 more
openaire   +2 more sources

Fed-batch mode in shake flasks by slow-release technique

Biotechnology and Bioengineering, 2006
Most industrial production processes are performed in fed-batch operational mode. In contrast, the screenings for microbial production strains are run in batch mode which results in completely different physiological conditions than relevant for production conditions. This may lead to wrong selections of strains.
Jochen Büchs, Doris Klee, H. Niederschulte, Markus Jeude, Barbara Dittrich, Christof Knocke, Tibor Anderlei   +6 more
openaire   +3 more sources

Improved shake-flask test for the screening of xanthan-producing microorganisms

World Journal of Microbiology & Biotechnology, 1993
Baffled 500 ml Erlenmeyer flasks were compared with conventional 2800 ml Fernbach flasks forXanthomonas campestris to produce xanthan. Bacterial growth rates were similar in both types of flask although the Fernbach flasks gave higher biomass concentrations. Xanthan production was similar in both types of flasks but different viscosities were attained.
Guadalupe Salcedo, Ma. Eugenia Ramírez, Celia Flores, Enrique Galindo   +3 more
openaire   +3 more sources

Closure effects on oxygen transfer and aerobic growth in shake flasks

Biotechnology and Bioengineering, 2006
AbstractOxygen mass transfer in shake flasks is an important aspect limiting the culture of aerobic microorganisms. In this work, mass transfer of oxygen through a closure and headspace of shake flasks is investigated. New equations for prediction of kGa in shake flasks with closures are introduced. Using Pseudomonas putida, microbial growth on glucose
Hossein Nikakhtari, Hossein Nikakhtari, Gordon A. Hill   +2 more
openaire   +2 more sources

Practices of shake-flask culture and advances in monitoring CO2 and O2

Applied Microbiology and Biotechnology, 2018
About 85 years have passed since the shaking culture was devised. Since then, various monitoring devices have been developed to measure culture parameters. O2 consumed and CO2 produced by the respiration of cells in shaking cultures are of paramount importance due to their presence in both the culture broth and headspace of shake flask.
Masato Takahashi, Hideki Aoyagi
openaire   +3 more sources