Results 61 to 70 of about 17,254 (263)

A non‐fluorescent immunohistochemistry method for measuring autophagy flux using MAP1LC3/LC3 and SQSTM1 as core markers

FEBS Open Bio, EarlyView.
We introduce an immunohistochemistry method to measure autophagy flux, highlighting the active degradation and recycling of cellular waste. This cost‐effective approach uses tissue samples to track key markers like LC3 and SQSTM1, revealing how cells maintain health or respond to diseases such as cancer. It bridges the gap between research and clinical
Shahla Shojaei, Amir Barzegar Behrooz, Marco Cordani, Mahmoud Aghaei, Negar Azarpira, Daniel J. Klionsky, Saeid Ghavami   +6 more
wiley   +1 more source

Soman induces endoplasmic reticulum stress and apoptosis of cerebral organoids via the GRP78‐ATF6‐CHOP signaling pathway

FEBS Open Bio, EarlyView.
Cerebral organoids were employed as a novel model to explore the neurotoxicity of soman. Soman inhibited acetylcholinesterase activity, increased cell apoptosis and upregulated endoplasmic reticulum (ER) stress markers glucose‐regulated protein 78 (GRP78), activating transcription factor 6 (ATF6) and C/EBP homologous protein (CHOP).
Yue Wei, Zhanbiao Liu, Jingjing Shi, Qian Jin, Wenqian Chen, Xuejun Chen, Liqin Li, Hui Chen   +7 more
wiley   +1 more source

Studies on Log Po/w of Quinoxaline di-N-Oxides: A Comparison of RP-HPLC Experimental and Predictive Approaches

Molecules, 2011
As reported in our previous papers, a series of quinoxaline-2-carboxamide 1,4-di-N-oxide derivatives were synthesized and studied as anti-tuberculosis agents. Here, the capability of the shake-flask method was studied and the retention time (expressed as
James A. Platts, Antonio Monge, Ignacio Aldana, Mauro Ravera, Enrique Torres, Silvia Pérez-Silanes, Elisabetta Gabano, Elsa Moreno   +7 more
doaj   +1 more source

Modeling bikaverin production by Fusarium oxysporum CCT7620 in shake flask cultures

Bioresources and Bioprocessing, 2020
Bikaverin is a fungal red pigment that presents antimicrobial and antitumor activities. Therefore, this substance could be used as an alternative additive in the food and pharmaceutical industries.
Marcela Colombo dos Santos, Mayra de Lima Mendonça, Juliano Lemos Bicas   +2 more
doaj   +1 more source

Artificial Receptor in Synthetic Cells Performs Transmembrane Activation of Proteolysis

Advanced Biology, EarlyView.
Transmembrane signaling is the hallmark of living cells and is among the highest challenges for the design of synthetic cells. Herein, an artificial receptor based on the chemistry of self‐immolative linkers is used to communicate information across the lipid bilayer, for transmembrane activation of enzymatic activity. Abstract The design of artificial,
Ane Bretschneider Søgaard, Kaja Borup Løvschall, Mireia Casanovas Montasell, Clara Bakkegaard Cramer, Pere Monge Marcet, Andreas Bøtker Pedersen, Josefine Hammer Jakobsen, Alexander N. Zelikin   +7 more
wiley   +1 more source

Vision-aided nonlinear control framework for shake table tests [PDF]

arXiv, 2023
The structural response under the earthquake excitations can be simulated by scaled-down model shake table tests or full-scale model shake table tests. In this paper, adaptive control theory is used as a nonlinear shake table control algorithm which considers the inherent nonlinearity of the shake table system and the Control-Structural Interaction ...
arxiv  

Finite-momentum Bose-Einstein condensates in shaken 2D square optical lattices [PDF]

Phys. Rev. A 84, 013607 (2011), 2011
We consider ultracold bosons in a 2D square optical lattice described by the Bose-Hubbard model. In addition, an external time-dependent sinusoidal force is applied to the system, which shakes the lattice along one of the diagonals. The effect of the shaking is to renormalize the nearest-neighbor hopping coefficients, which can be arbitrarily reduced ...
arxiv   +1 more source

Characterization and Optimization of Vesicle Properties in bioPISA: from Size Distribution to Post‐Assembly Loading

Advanced Biology, EarlyView.
The paper explores the creation and characterization of vesicles through biocatalytic Polymerization‐Induced Self‐Assembly (bioPISA), focusing on achieving size uniformity using centrifugation techniques. It examines the effects of stirring speed on vesicle morphology and analyses the internal polymer‐rich structure using fluorescence correlation ...
Andrea Belluati, Adrian Bloch, Kaloian Koynov, Mariana Müller Nieva, Mohadeseh Bagherabadi, Annette Andrieu‐Brunsen, Harald Kolmar, Nico Bruns   +7 more
wiley   +1 more source

Equilibrium solubility using shake-flask method of JM-20: a synthetic molecule with neuroprotective action [PDF]

Journal of Pharmacy & Pharmacognosy Research, 2020
Context: Drug solubility is one of the most important physicochemical properties for the development of new pharmaceutical products, which, in our study, is a new synthetic molecule, which has been shown to have a potential neuroprotective effect in rat.
Luis O. Martínez Álvarez, Pedro L. Alejo Cisneros, Rionaldo Forte Mesa, Livania Quiñones Hinojosa, Abel Mondelo Rodríguez, Lisandra García Borges, Hugo Guerra Menéndez, Ángela Tuero Iglesias, Estael Ochoa Rodríguez, Miguel A. Cabrera Pérez   +9 more
doaj  

Distinct Network Morphologies from In Situ Polymerization of Microtubules in Giant Polymer‐Lipid Hybrid Vesicles

Advanced Biology, EarlyView.
The successful in situ polymerization of microtubules in giant polymer‐lipid hybrid vesicles is illustrated. The microtubules formed in the giant hybrid vesicles exhibit different morphologies including lumenal network formation and membrane association. Abstract Creating artificial cells with a dynamic cytoskeleton, akin to those in living cells, is a
Paula De Dios Andres, Mousumi Akter, Cecilie Ryberg, Brigitte Städler, Allen P. Liu   +4 more
wiley   +1 more source