Results 131 to 139 of about 564 (139)

Characterization and humanization of VNARs targeting human serum albumin from the whitespotted bamboo shark (Chiloscyllium plagiosum)

International Journal of Biological Macromolecules
The Shark-derived immunoglobulin new antigen receptors (IgNARs) have gained increasing attention for their high solubility, exceptional thermal stability, and intricate sequence variation. In this study, we immunized whitespotted bamboo shark (Chiloscyllium plagiosum) to create phage display library of variable domains of IgNAR (VNARs) for screening ...
Weijie, Li, Mingliang, Chen, Tao, Wang, Xin, Feng, Xierui, Jiang, Xiaoning, Dong, Huan, Zhang, Xixiang, Tang, Rui, Tian, Yongyou, Zhang, Zengpeng, Li   +10 more
openaire   +2 more sources

Identification of nurse shark VNAR single-domain antibodies targeting the spike S2 subunit of SARS-CoV-2.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2023
SARS-CoV-2 is the etiological agent of the COVID-19 pandemic. Antibody-based therapeutics targeting the spike protein, specifically the S1 subunit or the receptor binding domain (RBD) of SARS-CoV-2, have gained attention due to their clinical efficacy in treating patients diagnosed with COVID-19.
Jesse, Buffington, Zhijian, Duan, Hyung Joon, Kwon, Jessica, Hong, Dan, Li, Mingqian, Feng, Hang, Xie, Mitchell, Ho   +7 more
openaire   +1 more source

Monoclonal Antibodies for the Identification and Purification of vNAR Domains and IgNAR Immunoglobulins from the Horn SharkHeterodontus francisci

Hybridoma, 2011
In addition to conventional antibodies, cartilaginous fish have evolved a distinctive type of immunoglobulin, designated as IgNAR, which lacks the light polypeptide chains and is composed entirely by heavy chains. IgNAR molecules can be manipulated by molecular engineering to produce the variable domain of a single heavy chain polypeptide (vNARs ...
Karla, Juarez, Gudrun, Dubberke, Pavel, Lugo, Friedrich, Koch-Nolte, Friedrich, Buck, Friedrich, Haag, Alexei, Licea   +6 more
openaire   +2 more sources

Construction of Histidine-Enriched Shark IgNAR Variable Domain Antibody Libraries for the Isolation of pH-Sensitive vNAR Fragments

2018
The adaptive immune system of sharks comprises a heavy chain-only antibody isotype, referred to as immunoglobulin new antigen receptor (IgNAR). Antigen binding in case of IgNAR antibodies is mediated by a single variable domain (vNAR). Due to their inherent beneficial biophysical properties, such as small size and high thermal stability combined with a
Doreen, Könning, Steffen, Hinz, Julius, Grzeschik, Christian, Schröter, Simon, Krah, Stefan, Zielonka, Harald, Kolmar   +6 more
openaire   +2 more sources

IgNAR characterization and gene loci identification in whitespotted bamboo shark (Chiloscyllium plagiosum) genome

Fish and Shellfish Immunology, 2023
bo zhong, Guodong Chen, Xiaofeng Jiang
exaly  

Ancient species offers contemporary therapeutics: an update on shark VNAR single domain antibody sequences, phage libraries and potential clinical applications

Antibody Therapeutics, 2020
Mitchell Ho
exaly  

KRM1 Targeting Shark VNARs Confer Broad Protection Against Enterovirus Infection

openaire   +1 more source

Synthesis of Shark VNAR Antibody Labelled Gold Nanoparticles for Potential Plasmodium Falciparum, Malaria Diagnosis Kit Development

Dhenmolly Arumugam, Nor Dyana Zakaria, Boon Hui Kok, Khairunisak Abdul Razak, Chiuan Yee Leow, Chiuan Herng Leow   +5 more
openaire   +1 more source

Swimming with sharks: expression, purification, and characterization of variable new antigen receptor (VNAR) mutant variants

openaire   +1 more source