Results 91 to 100 of about 237,743 (266)

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

Evolutionary Conserved Short Linear Motifs Provide Insights into the Cellular Response to Stress

Antioxidants, 2022
Short linear motifs (SLiMs) are evolutionarily conserved functional modules of proteins composed of 3 to 10 residues and involved in multiple cellular functions. Here, we performed a search for SLiMs that exert sequence similarity to two segments of alpha-fetoprotein (AFP), a major mammalian embryonic and cancer-associated protein.
Sergey P. Zavadskiy, Denis S. Gruzdov, Susanna S. Sologova, Alexander A. Terentiev, Nurbubu T. Moldogazieva   +4 more
openaire   +3 more sources

The leader proteins of Theiler’s virus and Boone cardiovirus use a combination of short linear motifs (SLiMs) to target RSK kinases to the nuclear pore complex

Journal of Virology
Unrelated pathogens, including viruses and bacteria, use a common short linear motif (SLiM) to interact with cellular kinases of the RSK (p90 S6 ribosomal kinase) family.
Belén Lizcano-Perret, Fanny Wavreil, Martin Veinstein, Camille Duflos, Romane Milcamps, Frédéric Sorgeloos, Thomas Michiels   +6 more
doaj   +1 more source

Potential of known and short prokaryotic protein motifs as a basis for novel peptide-based antibacterial therapeutics: a computational survey.

Frontiers in Microbiology, 2014
Short linear motifs are functional stretches of protein sequence that are of crucial importance for numerous biological processes by mediating protein-protein interactions.
Heini eRuhanen, Heini eRuhanen, Heini eRuhanen, Daniel eHurley, Daniel eHurley, Daniel eHurley, Ambarnil eGhosh, Kevin T O'Brien, Kevin T O'Brien, Kevin T O'Brien, Catrióna R Johnston, Denis Colum Shields, Denis Colum Shields, Denis Colum Shields   +13 more
doaj   +1 more source

Interactomic affinity profiling by holdup assay: Acetylation and distal residues impact the PDZome-binding specificity of PTEN phosphatase.

PLoS ONE, 2020
Protein domains often recognize short linear protein motifs composed of a core conserved consensus sequence surrounded by less critical, modulatory positions.
Pau Jané, Gergő Gógl, Camille Kostmann, Goran Bich, Virginie Girault, Célia Caillet-Saguy, Pascal Eberling, Renaud Vincentelli, Nicolas Wolff, Gilles Travé, Yves Nominé   +10 more
doaj   +1 more source

Fast and accurate discovery of degenerate linear motifs in protein sequences. [PDF]

PLoS ONE, 2014
Linear motifs mediate a wide variety of cellular functions, which makes their characterization in protein sequences crucial to understanding cellular systems.
Abdellali Kelil, Benjamin Dubreuil, Emmanuel D Levy, Stephen W Michnick   +3 more
doaj   +1 more source

Modeling Cell-to-Cell Communication Networks Using Response-Time Distributions. [PDF]

, 2018
Cell-to-cell communication networks have critical roles in coordinating diverse organismal processes, such as tissue development or immune cell response. However, compared with intracellular signal transduction networks, the function and engineering
Altschuler, Steven J, Thurley, Kevin, Wu, Lani F   +2 more
core   +2 more sources

A methionine‐lined active site governs carbocation stabilization and product specificity in a bacterial terpene synthase

FEBS Letters, EarlyView.
This study reveals a unique active site enriched in methionine residues and demonstrates that these residues play a critical role by stabilizing carbocation intermediates through novel sulfur–cation interactions. Structure‐guided mutagenesis further revealed variants with significantly altered product profiles, enhancing pseudopterosin formation. These
Marion Ringel, Carl P. O. Helmer, Shani Zev, Ronja Driller, Emily Buhr, Markus Reinbold, Renana Schwartz, Gabriel Foley, Mikael Boden, Daniel Garbe, Gerhard Schenk, Dan Thomas Major, Bernhard Loll, Thomas Brück   +13 more
wiley   +1 more source

Learning a Hybrid Architecture for Sequence Regression and Annotation

, 2015
When learning a hidden Markov model (HMM), sequen- tial observations can often be complemented by real-valued summary response variables generated from the path of hid- den states. Such settings arise in numerous domains, includ- ing many applications in
Carin, Lawrence, Hartemink, Alexander J., Henao, Ricardo, Zhang, Yizhe, Zhong, Jianling   +4 more
core   +2 more sources

AAA+ protein unfoldases—the Moirai of the proteome

FEBS Letters, EarlyView.
AAA+ unfoldases are essential molecular motors that power protein degradation and disaggregation. This review integrates recent cryo‐electron microscopy (cryo‐EM) structures and single‐molecule biophysical data to reconcile competing models of substrate translocation.
Stavros Azinas, Marta Carroni
wiley   +1 more source