Results 31 to 40 of about 90,397 (258)

General Approach to Analysis of Polymorphic Short Tandem Repeat Loci

BioTechniques, 1996
Polymerase chain reaction amplification products of 22 known polymorphic short tandem repeat (STR) loci were subjected to denaturing polyacrylamide gel electrophoresis and detected using a silver staining method.
Cynthia J. Sprecher, Christoph Puers, Ann M. Lins, James W. Schumm   +3 more
doaj   +1 more source

Genetic analysis of DXS101and DXS6807 short tandem repeat (X-STR) in samples of Iraqi Arab male population

مجلة مركز بحوث التقنيات الاحيائية, 2020
Back ground: X-chromosomal short tandem repeats (X-STRs) have assured to be informative and particular role in complex relationship testing. DXS6807 known as tetra nucleotides polymorphism representing eight alleles of 251-275 bp in length.
Hayder Allawi Khaleefah , Salwa Jaber Abdullah Al-Awadi, Zaid Nsaif Abbas Al-Tameemi   +2 more
doaj   +1 more source

Systematic profiling of short tandem repeats in the cattle genome [PDF]

Genome Biology and Evolution, 2016
Short tandem repeats (STRs), or microsatellites, are genetic variants with repetitive 2–6 base pair motifs in many mammalian genomes. Using high-throughput sequencing and experimental validations, we systematically profiled STRs in five Holsteins.
Xu, Lingyang, Haasl, Ryan J., Sun, Jiajie, Zhou, Yang, Bickhart, Derek M., Li, Junya, Song, Jiuzhou, Sonstegard, Tad S., Van Tassell, Curtis P., Lewin, Harris A., Liu, George E.   +10 more
openaire   +2 more sources

Comprehensive DNA profiling strategy for patient-derived xenograft model authentication in the J-PDX library

Scientific Reports
Patient-derived xenograft models are crucial in cancer research, although authentication methods remain limited. This study developed a comprehensive DNA profiling strategy for patient-derived xenograft quality control in the J-PDX Library by comparing ...
Tomomi Yoshino, Shigehiro Yagishita, Mami Takahashi, Chiaki Ushie, Ako Takahashi, Yasushi Yatabe, Shinji Kohsaka, Toshihide Ueno, Hanako Ono, Kouya Shiraishi, Akinobu Hamada   +10 more
doaj   +1 more source

Short tandem repeat typing technologies used in human identity testing

BioTechniques, 2007
Short tandem repeat (STR) typing methods are widely used today for human identity testing applications including forensic DNA analysis. Following multiplex PCR amplification, DNA samples containing the length-variant STR alleles are typically separated ...
John M. Butler
doaj   +1 more source

The effect of temperature and storage time of cuccal swabs on FGA and D13S317 loci with the STR PCR method

Jurnal Teknologi Laboratorium, 2020
The samples used for forensic DNA analysis in living individuals are usually blood and buccal swabs, however, blood collection requires an invasive method that can cause discomfort, thus a buccal swab can be a good choice for individuals examined ...
Indah Nuraini Masjkur, Evy Arfianti, Ahmad Yudianto, Abdul Hadi Furqoni, Qurrota A’yun   +4 more
doaj   +1 more source

Tau acetylation at K331 has limited impact on tau pathology in vivo

FEBS Letters, EarlyView.
We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto, Yukio Matsuba, Mika Takahashi, Takaomi C. Saido   +3 more
wiley   +1 more source

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

Mapping the landscape of tandem repeat variability by targeted long read single molecule sequencing in familial X-linked intellectual disability

BMC Medical Genomics, 2018
Background The etiology of more than half of all patients with X-linked intellectual disability remains elusive, despite array-based comparative genomic hybridization, whole exome or genome sequencing.
Alena Zablotskaya, Hilde Van Esch, Kevin J. Verstrepen, Guy Froyen, Joris R. Vermeesch   +4 more
doaj   +1 more source

TRAIL‐PEG‐Apt‐PLGA nanosystem as an aptamer‐targeted drug delivery system potential for triple‐negative breast cancer therapy using in vivo mouse model

Molecular Oncology, EarlyView.
Aptamers are used both therapeutically and as targeting agents in cancer treatment. We developed an aptamer‐targeted PLGA–TRAIL nanosystem that exhibited superior therapeutic efficacy in NOD/SCID breast cancer models. This nanosystem represents a novel biotechnological drug candidate for suppressing resistance development in breast cancer.
Gulen Melike Demirbolat, Aslihan Kucuk, Omer Erdogan, Samed Ozer, Bensu Kozan, Tugba Cuceli, Erkan Gumus, Evrim Cevik, Ozge Cevik   +8 more
wiley   +1 more source