Сахарный диабет, 2010 Aim. To measure concentration of carbohydrate-containing biopolymers in liver and gastric mucosa of rats with alloxan diabetes and varying stressresistance. Materials and methods. The ?open field?
Svetlana Vladimirovna Protasova +2 more
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Visualization of sialoglycoproteins in polyacrylamide gels by acidic ninhydrin reaction. [PDF]
, 1990 A new method for staining sialoglycoproteins in polyacrylamide gel after disc electrophoresis is described. The method utilizes the reaction of sialic acids with an acidic ninhydrin reagent which yields a stable color with an absorption maximum at 470 nm.
Yao, Kenzabroh +5 more
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Rapid chemical de-N-glycosylation and derivatization for liquid chromatography of immunoglobulin N-linked glycans. [PDF]
PLoS ONE, 2018 Glycan analysis may result in exploitation of glycan biomarkers and evaluation of heterogeneity of glycosylation of biopharmaceuticals. For N-linked glycan analysis, we investigated alkaline hydrolysis of the asparagine glycosyl carboxamide of ...
Akihiko Kameyama +2 more
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Žurnal Grodnenskogo Gosudarstvennogo Medicinskogo Universiteta, 2018 Objectives: To assess the biochemical indicators of the connective tissue of rat periodontium (collagen, proteoglycans, glycoproteins) in local gum damage associated with lipopolysaccharide-induced systemic inflammatory response (SIR).
Yelinskaya A. N. +2 more
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Inhibitory effects of Trypanosoma cruzi sialoglycoproteins on CD4+ T cells are associated with increased susceptibility to infection. [PDF]
PLoS ONE, 2013 BackgroundThe Trypanosoma cruzi infection is associated with severe T cell unresponsiveness to antigens and mitogens characterized by decreased IL-2 synthesis. Trypanosoma cruzi mucin (Tc Muc) has been implicated in this phenomenom.
Marise Pinheiro Nunes +14 more
doaj +1 more source
The use of monoclonal antibodies to quantify the levels of sialoglycoproteins α and δ and variant sialoglycoproteins in human erythrocyte membranes [PDF]
Biochemical Journal, 1986 By using radioiodinated monoclonal antibodies we have estimated that there are about 600 000 copies of sialoglycoprotein alpha (synonym glycophorin A) and 80 000 copies of sialoglycoprotein delta (synonym glycophorin B) per normal human erythrocyte. Erythrocytes expressing the product of only one alpha gene contain about 300 000 copies of alpha/cell ...
A H, Merry +4 more
openaire +2 more sources
Bacterial sialoglycosidases in Virulence and Pathogenesis
Pathogens, 2019 Human oral microbiome and dysbiotic infections have been recently evidently identified. One of the major reasons for such dysbiosis is impairment of the immune system.
Preethi Sudhakara +2 more
doaj +1 more source
Scientific Reports, 2022 Sialylation of cell surface glycans plays an essential role in cell–cell interaction and communication of cells with their microenvironment. Among the tools that have been developed for the study of sialylation in living cells, metabolic oligosaccharide ...
Jodie Scache +6 more
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Profiling cell surface sialoglycoproteins via the bio-orthogonal chemical reporter strategy combined with quantitative shotgun proteomics. [PDF]
, 2014 The strategy consists of six steps: 1) metabolic labeling of cells with Ac4ManNAz; 2) chemoselective conjugation of azide-labeled glycans with a biotin-linked phosphine; 3) cell lysis; 4) affinity enrichment of the biotin-tagged proteins on streptavidin ...
Steven A. Goldman (652727) +15 more
core +1 more source
Changes in the expression of cell surface sialoglycoproteins during transition of human monocytes into macrophages [PDF]
, 1986 Cell surface sialoglycoproteins of human mononuclear phagocytes in different maturation stages were labelled by the periodate/borohydride method and separated by SDS-polyacrylamide gel electrophoresis.
Risto Penttinen +5 more
core +1 more source