Affinity purification of biotinylated cell surface sialoglycoproteins. [PDF]
U373 MG cells, primary GBM cells (SIDs), adult (AL) and fetal (ASG6 and ASG7) astrocytes, and neural progenitor cells (NPC) were metabolically labelled with 25 µM Ac4ManNAz for 2 days and conjugated with 50 µM biotin-phosphine capture reagent.
Steven A. Goldman (652727) +15 more
core +1 more source
Sialoglycoproteins and N-glycans from secreted exosomes of ovarian carcinoma cells.
Exosomes consist of vesicles that are secreted by several human cells, including tumor cells and neurons, and they are found in several biological fluids.
Cristina Escrevente +6 more
doaj +1 more source
Fractionation of sialoglycoproteins on an immobilized sialic acid-binding lectin [PDF]
Carcinoscorpin, the sialic acid-binding lectin from the horseshoe crab Carcinoscorpius rotunda cauda, has been immobilized using Sepharose. The immobilized lectin is shown to resolve the isoenzymes of alkaline phosphatase from sheep brain based on the ...
Dorai, D. Thambi +2 more
core +1 more source
Mobilization of lymphoblasts from Bone Marrow to Peripheral Blood in Childhood Acute Lymphoblastic Leukaemia: Role of 9-O-Acetylated Sialoglycoproteins [PDF]
Childhood acute lymphoblastic leukaemia is characterized by aberrant proliferation and accumulation of malignant lymphoblasts in bone marrow (BM), followed by their migration into circulation.
Reinhard Vlasak +16 more
core +1 more source
Sialoglycoproteins of ejaculated human spermatozoa and seminal plasma [PDF]
Ejaculated human sperm were found to possess three major sialoglycoproteins with molecular weights of 30 000, 14 000 and 12 000 and one minor species of 18 000. Liquefied seminal plasma from normal donors contain two major sialoglycoproteins with molecular weights of 17 000 and 15 000 and two minor species of 70 000 and 54 000.
C, Wongkome, M, Chulavatnatol
openaire +2 more sources
ABSTRACT Preeclampsia is a severe gestational complication whose molecular pathogenesis remains poorly understood despite extensive research. It is now recognized that both maternal and fetal cells contribute to disease progression. Notably, single‐cell RNA sequencing of cord blood cells from preeclamptic pregnancies has not been previously ...
Polina Vishnyakova +14 more
wiley +1 more source
Top 52 cell surface sialoglycoproteins overexpressed in GBM tumor cells. [PDF]
aProteomic analysis of differential sialoglycoprotein expression in glioblastoma multiform (GBM) cells compared to astrocytes or neural progenitor cells (NPCs).
Steven A. Goldman (652727) +15 more
core +1 more source
This manuscript describes the cultivation of viable microvessels from cryopreserved human brain tissue. When embedded in hydrogels and cultured in microfluidic devices, these microvessels exhibit complex architectures reminiscent of arterioles and capillaries, can be perfused, and display intact barrier function. Collectively, these results demonstrate
Brian J. O'Grady +5 more
wiley +1 more source
How Does Biological Sex Impact Mucosal Bacterial Infection? Mucosal Defenses and Bacterial Detection
ABSTRACT Urinary tract infections possess substantial sex disparities in the incidence, immune response, and progression of infection. Some of these distinctions may be due to sexual dimorphism in mucosal barriers or sex differences in the initial immune response to infection.
Laura Ramirez Finn, Molly A. Ingersoll
wiley +1 more source
Generating golden Syrian hamsters with conditional alleles via zygote microinjection of CRISPR/Cas9
We established the first conditional knockout (cKO) model in the golden Syrian hamster by CRISPR/Cas9‐mediated genome editing. Cas9 protein, two sgRNAs, and a donor plasmid carrying loxP‐flanked exon 2 of the ApoF gene were microinjected into one‐cell embryos. The floxed allele was efficiently generated (up to 27%) and transmitted through the germline.
Wei Chen +16 more
wiley +1 more source

