Minimal Genome, Maximal Effect: How Polyomavirus Genomes Are Capable of Complex Pathogenesis [PDF]
VirusesThe Polyomaviridae family contains members known for achieving high seroprevalence within their target species despite a limited genomic economy. Minimalism, by definition, allows for the clarification and streamlining of purpose via the removal of ...
Michaela Lano, Barry Milavetz
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Combinatorial expression of cell cycle regulators is more suitable for immortalization than oncogenic methods in dermal papilla cells [PDF]
iScience, 2021 journal ...
94923 +17 more
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Efficient Establishment of Pig Embryonic Fibroblast Cell Lines with Conditional Expression of the Simian Vacuolating Virus 40 Large T Fragment [PDF]
Bioscience, Biotechnology and Biochemistry, 2012 The pig is an important animal for both agricultural and medical purposes. However, the number of pig-derived cell lines is relatively limited when compared with mouse- and human-derived lines. We established in this study a retroviral conditional expression system for the Simian vacuolating virus 40 large T fragment (SV40T) which allowed us to ...
Tomokazu Fukuda +2 more
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Advances in experimental animal models of hepatocellular carcinoma. [PDF]
Cancer Med, 2023 Animal models are crucial for basic research, which is conducive to studying pathogenesis and drug screening more conveniently and effectively. An appropriate animal model can better reflect disease occurrence and development, and the process of anti‐tumor immune response in the human body.
Li J, Wang X, Ren M, He S, Zhao Y.
europepmc +2 more sources
Immortalization effect of SV40T lentiviral vectors on canine corneal epithelial cells [PDF]
BMC Veterinary Research, 2022 Background Primary canine corneal epithelial cells (CCECs) easily become senescent, and cell proliferation is limited. Therefore, sampling for experimentation requires a large number of animals, which is problematic in terms of animal welfare and fails ...
Long Guo +8 more
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Multiplication and Cytopathogenicity of Simian Vacuolating Virus 40 in Cultures of Human Tissues.
Experimental Biology and Medicine, 1962 SummaryMultiplication of SV40 virus has been demonstrated in primary cultures of several human tissues. The virus multiplied without cytopathic effect during the first passage in these systems. In subsequent passages in kidney cell cultures, CPE was noted which included nuclear changes, increased cell proliferation and necrosis.
H M Shein, John F Enders
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In Vitro Endothelialization Test of Biomaterials Using Immortalized Endothelial Cells. [PDF]
PLoS ONE, 2016 Functionalizing biomaterials with peptides or polymers that enhance recruitment of endothelial cells (ECs) can reduce blood coagulation and thrombosis.
Ken Kono +6 more
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Microbial Regulation of p53 Tumor Suppressor. [PDF]
PLoS Pathogens, 2015 p53 tumor suppressor has been identified as a protein interacting with the large T antigen produced by simian vacuolating virus 40 (SV40). Subsequent research on p53 inhibition by SV40 and other tumor viruses has not only helped to gain a better ...
Alexander I Zaika +3 more
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Genetic characteristics of the human hepatic stellate cell line LX-2. [PDF]
PLoS ONE, 2013 The human hepatic cell line LX-2 has been described as tool to study mechanisms of hepatic fibrogenesis and the testing of antifibrotic compounds. It was originally generated by immortalisation with the Simian Vacuolating Virus 40 (SV40) transforming (T)
Ralf Weiskirchen +10 more
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Animals, 2022 The E2F family of transcription factor is divided into activators and repressors that control cell proliferation. Bovine mammary epithelial cells (BMECs) can be immortalized using human papillomavirus 16 E6E7 (HPV16 E6E7) and simian vacuolating virus 40 ...
Yihui Zhang +3 more
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