We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Semantic and fiducial-aided graph simultaneous localization and mapping (SF-GraphSLAM) for robotic in-space assembly and servicing of large truss structures. [PDF]
Chapin S, Chapin W, Komendera E.
europepmc +1 more source
Automatic Identification System (AIS) Dynamic Data Integrity Monitoring and Trajectory Tracking Based on the Simultaneous Localization and Mapping (SLAM) Process Model. [PDF]
Jaskólski K +4 more
europepmc +1 more source
Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus
BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima +7 more
wiley +1 more source
Multi-Robot Cooperative Simultaneous Localization and Mapping Algorithm Based on Sub-Graph Partitioning. [PDF]
Xu W, Chen Y, Liu S, Nie A, Chen R.
europepmc +1 more source
Real-Time Expanded Field-of-View for Minimally Invasive Surgery Using Multi-Camera Visual Simultaneous Localization and Mapping. [PDF]
Afifi A +3 more
europepmc +1 more source
Simultaneous localization, mapping and moving object tracking
LOCALIZATION, mapping and moving object tracking serve as the basis for scene understanding, which is a key prerequisite for making a robot truly autonomous.
Chieh-chih Wang
core
Heterotropic regulation and negative homotropic cooperativity
We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea +5 more
wiley +1 more source
Enhanced Simultaneous Localization and Mapping Algorithm Based on Deep Learning for Highly Dynamic Environment. [PDF]
Lu Y, Wang H, Sun J, Zhang JA.
europepmc +1 more source
Using peripheral blood for determining B‐cell or T‐cell clonality is more reliable when we use cell‐free RNA (cfRNA) because cells release blood significantly more RNA than DNA. Next‐generation sequencing (NGS) of cfRNA allows us to evaluate fragment cfRNA and evaluate clonality reliably without the need for prior determination of the specific dominant
Adam Albitar +11 more
wiley +1 more source

